Effects of catechin on menadione-induced cytotoxicity / Widiayanna A. Rahim

The main objective of this study was to investigate the cytoprotective effects of catechin in menadione-induced cytotoxicity in both WRL 68 and Hep G2 cell lines. In order to achieve those objectives, the dose response relationships of menadione and catechin concentrations and their cytotoxic effect...

Full description

Saved in:

Bibliographic Details
Main Author:	A. Rahim, Widiayanna
Format:	Thesis
Language:	English
Published:	2008
Subjects:	RM Therapeutics Pharmacology RS Pharmacy and materia medica
Online Access:	https://ir.uitm.edu.my/id/eprint/101023/1/101023.PDF
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.101023
record_format	uketd_dc
spelling	my-uitm-ir.1010232024-08-26T04:25:13Z Effects of catechin on menadione-induced cytotoxicity / Widiayanna A. Rahim 2008 A. Rahim, Widiayanna RM Therapeutics. Pharmacology RS Pharmacy and materia medica The main objective of this study was to investigate the cytoprotective effects of catechin in menadione-induced cytotoxicity in both WRL 68 and Hep G2 cell lines. In order to achieve those objectives, the dose response relationships of menadione and catechin concentrations and their cytotoxic effects in each of the cell types were first explored. This allowed the determination of the median inhibitory concentration (IC50) for menadione and catechin. In order to assess cytoprotective effects of catechin, cells were then treated with menadione and catechin concomitantly for 24 h, after which cell viability was measured by the MTS assay. The IC50 values for menadione in WRL 68 and Hep G2 cell lines were 31.62±0.42µM and 3 I .62±0.86µM, respectively. IC50 values for catechin in WRL 68 and Hep G2 cell lines were 223.87±7.66 µMand 199.52±14.05 µM, respectively. In the cytoprotective studies, neither menadione ( I 5µM and 30 µM) nor catechin (30µM and 50µM) elicited a significant decrease in cell viability in WRL 68 cells. Thus, no cytoprotective effects by catechin against menadione induced cytotoxicity could be proven. A similar study in Hep G2 cells showed menadione ( I 5µM and 30µM) to elicit a significant decrease in cell viability compared to controls while catechin (30µM and 50µM) was without affect. Concomitant administration of menadione and catechin failed to show protection of Hep G2 cells against menadione's cytotoxicity by catechin. In summary, dose-response relationships for the cytotoxicity of menadione and catechin were obtained in WRI 68 and Hep G2 cells. Catechin (30µM and 50µM) failed to show cytoprotection of these cell lines against menadione's ( I 5µM and 30µM) cytotoxicity. 2008 Thesis https://ir.uitm.edu.my/id/eprint/101023/ https://ir.uitm.edu.my/id/eprint/101023/1/101023.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Adam, Aishah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Adam, Aishah
topic	RM Therapeutics Pharmacology RS Pharmacy and materia medica
spellingShingle	RM Therapeutics Pharmacology RS Pharmacy and materia medica A. Rahim, Widiayanna Effects of catechin on menadione-induced cytotoxicity / Widiayanna A. Rahim
description	The main objective of this study was to investigate the cytoprotective effects of catechin in menadione-induced cytotoxicity in both WRL 68 and Hep G2 cell lines. In order to achieve those objectives, the dose response relationships of menadione and catechin concentrations and their cytotoxic effects in each of the cell types were first explored. This allowed the determination of the median inhibitory concentration (IC50) for menadione and catechin. In order to assess cytoprotective effects of catechin, cells were then treated with menadione and catechin concomitantly for 24 h, after which cell viability was measured by the MTS assay. The IC50 values for menadione in WRL 68 and Hep G2 cell lines were 31.62±0.42µM and 3 I .62±0.86µM, respectively. IC50 values for catechin in WRL 68 and Hep G2 cell lines were 223.87±7.66 µMand 199.52±14.05 µM, respectively. In the cytoprotective studies, neither menadione ( I 5µM and 30 µM) nor catechin (30µM and 50µM) elicited a significant decrease in cell viability in WRL 68 cells. Thus, no cytoprotective effects by catechin against menadione induced cytotoxicity could be proven. A similar study in Hep G2 cells showed menadione ( I 5µM and 30µM) to elicit a significant decrease in cell viability compared to controls while catechin (30µM and 50µM) was without affect. Concomitant administration of menadione and catechin failed to show protection of Hep G2 cells against menadione's cytotoxicity by catechin. In summary, dose-response relationships for the cytotoxicity of menadione and catechin were obtained in WRI 68 and Hep G2 cells. Catechin (30µM and 50µM) failed to show cytoprotection of these cell lines against menadione's ( I 5µM and 30µM) cytotoxicity.
format	Thesis
qualification_level	Bachelor degree
author	A. Rahim, Widiayanna
author_facet	A. Rahim, Widiayanna
author_sort	A. Rahim, Widiayanna
title	Effects of catechin on menadione-induced cytotoxicity / Widiayanna A. Rahim
title_short	Effects of catechin on menadione-induced cytotoxicity / Widiayanna A. Rahim
title_full	Effects of catechin on menadione-induced cytotoxicity / Widiayanna A. Rahim
title_fullStr	Effects of catechin on menadione-induced cytotoxicity / Widiayanna A. Rahim
title_full_unstemmed	Effects of catechin on menadione-induced cytotoxicity / Widiayanna A. Rahim
title_sort	effects of catechin on menadione-induced cytotoxicity / widiayanna a. rahim
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2008
url	https://ir.uitm.edu.my/id/eprint/101023/1/101023.PDF
_version_	1811769120154189824

Effects of catechin on menadione-induced cytotoxicity / Widiayanna A. Rahim

Similar Items