Amplification of human VKOR gene using PCR for use in cloning & expression / Nurul Ashikin Mustafa

Mutations of human VKOR gene leading to warfarin resistance in certain patients and polymorphism which is associated with a significantly higher risk of aortic calcification in certain patients have been identified. By identification of VKOR gene defects, prevention or treatment inititative such as...

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Main Author:	Mustafa, Nurul Ashikin
Format:	Thesis
Language:	English
Published:	2008
Subjects:	DNA Deoxyribonucleic acids Genetic engineering applications
Online Access:	https://ir.uitm.edu.my/id/eprint/101028/1/101028.PDF
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id	my-uitm-ir.101028
record_format	uketd_dc
spelling	my-uitm-ir.1010282024-08-26T04:59:27Z Amplification of human VKOR gene using PCR for use in cloning & expression / Nurul Ashikin Mustafa 2008 Mustafa, Nurul Ashikin DNA. Deoxyribonucleic acids RM Therapeutics. Pharmacology Genetic engineering applications Mutations of human VKOR gene leading to warfarin resistance in certain patients and polymorphism which is associated with a significantly higher risk of aortic calcification in certain patients have been identified. By identification of VKOR gene defects, prevention or treatment inititative such as gene therapy may be initiated for affected patients. PCR is a technique to amplify specific DNA sequences in vitro. PCR is able to rapidly amplify a specific region of a single DNA molecule to yield sufficient quantities that can be cloned, sequenced, or analysed by sequences mapping . The aim of this study is to amplify human VKOR gene using PCR for use in cloning and expression. In this study, specific primers flanking the complete VKOR coding region was designed. The specificity of the primers were evaluated using Oligo Explorer 1.2 Software. The PCR was then performed to detect the VKOR gene. The PCR conditions were optimized by varying the PCR temperature and time. In order to prove the target PCR products were obtained after amplification, the PCR products were separated by gel electrophoresis and visualized under UV transilluminator. The band of interest which was 492 base pairs in size were obtained after human VKOR gene amplification was performed. The amplicon can then be used for cloning and expression of YKOR enzyme. However, more studies need to be performed for more specific amplification without non-specific co-amplification. 2008 Thesis https://ir.uitm.edu.my/id/eprint/101028/ https://ir.uitm.edu.my/id/eprint/101028/1/101028.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Salleh, Mohd Zaki
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Salleh, Mohd Zaki
topic	DNA Deoxyribonucleic acids DNA Deoxyribonucleic acids Genetic engineering applications
spellingShingle	DNA Deoxyribonucleic acids DNA Deoxyribonucleic acids Genetic engineering applications Mustafa, Nurul Ashikin Amplification of human VKOR gene using PCR for use in cloning & expression / Nurul Ashikin Mustafa
description	Mutations of human VKOR gene leading to warfarin resistance in certain patients and polymorphism which is associated with a significantly higher risk of aortic calcification in certain patients have been identified. By identification of VKOR gene defects, prevention or treatment inititative such as gene therapy may be initiated for affected patients. PCR is a technique to amplify specific DNA sequences in vitro. PCR is able to rapidly amplify a specific region of a single DNA molecule to yield sufficient quantities that can be cloned, sequenced, or analysed by sequences mapping . The aim of this study is to amplify human VKOR gene using PCR for use in cloning and expression. In this study, specific primers flanking the complete VKOR coding region was designed. The specificity of the primers were evaluated using Oligo Explorer 1.2 Software. The PCR was then performed to detect the VKOR gene. The PCR conditions were optimized by varying the PCR temperature and time. In order to prove the target PCR products were obtained after amplification, the PCR products were separated by gel electrophoresis and visualized under UV transilluminator. The band of interest which was 492 base pairs in size were obtained after human VKOR gene amplification was performed. The amplicon can then be used for cloning and expression of YKOR enzyme. However, more studies need to be performed for more specific amplification without non-specific co-amplification.
format	Thesis
qualification_level	Bachelor degree
author	Mustafa, Nurul Ashikin
author_facet	Mustafa, Nurul Ashikin
author_sort	Mustafa, Nurul Ashikin
title	Amplification of human VKOR gene using PCR for use in cloning & expression / Nurul Ashikin Mustafa
title_short	Amplification of human VKOR gene using PCR for use in cloning & expression / Nurul Ashikin Mustafa
title_full	Amplification of human VKOR gene using PCR for use in cloning & expression / Nurul Ashikin Mustafa
title_fullStr	Amplification of human VKOR gene using PCR for use in cloning & expression / Nurul Ashikin Mustafa
title_full_unstemmed	Amplification of human VKOR gene using PCR for use in cloning & expression / Nurul Ashikin Mustafa
title_sort	amplification of human vkor gene using pcr for use in cloning & expression / nurul ashikin mustafa
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2008
url	https://ir.uitm.edu.my/id/eprint/101028/1/101028.PDF
_version_	1811769121158725632

Amplification of human VKOR gene using PCR for use in cloning & expression / Nurul Ashikin Mustafa

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