The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan
NMDA-Rl subunit is important in synaptic plasticity and learning process. The aim of the present study was to determine the effect of HAB10R12 extract on the expression of N-Methyl-D-Aspartate Receptor-I subunit gene in mice brain after sub-acute exposure of HAB10R12. Thirty mice were randomly divid...
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my-uitm-ir.1010862024-09-28T23:32:53Z The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan 2008 Kasiman @ Kamisan, Norhidayah Pharmaceutical technology Pharmaceutical chemistry NMDA-Rl subunit is important in synaptic plasticity and learning process. The aim of the present study was to determine the effect of HAB10R12 extract on the expression of N-Methyl-D-Aspartate Receptor-I subunit gene in mice brain after sub-acute exposure of HAB10R12. Thirty mice were randomly divided into five groups which consist of normal saline, memantine, 5 mg/ml/kg, l O mg/ml/kg and 20 mg/ml/kg of HAB10R12 treatments. All the groups of mice were treated by intraperitoneal (IP) administration for 14 days. The mice were sacrificed at day I 5 and the brains were removed. RNA was extracted from the brains by using SY Total RNA Isolation System (Promega). QuantiFast SYBR Green RT-PCR Kit (Qiagen) was used to quantify the expression of NMDA-R1 subunit gene by using comparative threshold method that was recorded by Rotor-Gene 600 Series Software I. 7. The result showed that administration of 5mg/ml/kg HAB10R12 dose caused up-regulation of NMDA-R1 subunit gene in mice. In contrast, administration of 10mg/ml/kg HAB10R12 dose to mice cause down regulation of NMDA-R1 subunit gene. In conclusion, HAB10R12 might affect the expression ofNMDA-R1 subunit gene in mice brain after sub-acute exposure. 2008 Thesis https://ir.uitm.edu.my/id/eprint/101086/ https://ir.uitm.edu.my/id/eprint/101086/1/101086.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy |
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Pharmaceutical technology Pharmaceutical chemistry Kasiman @ Kamisan, Norhidayah The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan |
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NMDA-Rl subunit is important in synaptic plasticity and learning process. The aim of the present study was to determine the effect of HAB10R12 extract on the expression of N-Methyl-D-Aspartate Receptor-I subunit gene in mice brain after sub-acute exposure of HAB10R12. Thirty mice were randomly divided into five groups which consist of normal saline, memantine, 5 mg/ml/kg, l O mg/ml/kg and 20 mg/ml/kg of HAB10R12 treatments. All the groups of mice were treated by intraperitoneal (IP) administration for 14 days. The mice were sacrificed at day I 5 and the brains were removed. RNA was extracted from the brains by using SY Total RNA Isolation System (Promega). QuantiFast SYBR Green RT-PCR Kit (Qiagen) was used to quantify the expression of NMDA-R1 subunit gene by using comparative threshold method that was recorded by Rotor-Gene 600 Series Software I. 7. The result showed that administration of 5mg/ml/kg HAB10R12 dose caused up-regulation of NMDA-R1 subunit gene in mice. In contrast, administration of 10mg/ml/kg HAB10R12 dose to mice cause down regulation of NMDA-R1 subunit gene. In conclusion, HAB10R12 might affect the expression ofNMDA-R1 subunit gene in mice brain after sub-acute exposure. |
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Bachelor degree |
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Kasiman @ Kamisan, Norhidayah |
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Kasiman @ Kamisan, Norhidayah |
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Kasiman @ Kamisan, Norhidayah |
title |
The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan |
title_short |
The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan |
title_full |
The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan |
title_fullStr |
The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan |
title_full_unstemmed |
The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan |
title_sort |
effects of sub-acute exposure of hab10r12 ethanolic extract on the expression of n-methyl-d-aspartate receptor-i subunit gene in mice brain / norhidayah kasiman @ kamisan |
granting_institution |
Universiti Teknologi MARA (Kampus Puncak Alam) |
granting_department |
Faculty of Pharmacy |
publishDate |
2008 |
url |
https://ir.uitm.edu.my/id/eprint/101086/1/101086.PDF |
_version_ |
1811769128480931840 |