Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi

The study was done to identify the effect of culture condition on metabolite production by fungal strain 4PL2. fungi strain 4PL2 was isolated from plant of Pandanus herbaceous which was collected from Port Dickson, Negeri Sembilan in 2006. the study was performed by growing the pure culture of fungi...

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Main Author:	Mohd Abidi, Nor Azizah
Format:	Thesis
Language:	English
Published:	2008
Subjects:	Pharmaceutical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/101094/1/101094.PDF
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id	my-uitm-ir.101094
record_format	uketd_dc
spelling	my-uitm-ir.1010942024-09-28T23:34:54Z Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi 2008 Mohd Abidi, Nor Azizah Pharmaceutical chemistry The study was done to identify the effect of culture condition on metabolite production by fungal strain 4PL2. fungi strain 4PL2 was isolated from plant of Pandanus herbaceous which was collected from Port Dickson, Negeri Sembilan in 2006. the study was performed by growing the pure culture of fungi strain 4PL2 in different media, different strength and different incubation period. The pure culture of 4PL2 was then incubated for 2 weeks, 3 weeks and 4 weeks. Then the myceliums of fungi strain 4PL2 were subjected to extraction process by using ethyl acetate as a solvent. After obtaining the pure extracts, then subjected to antimicrobial assay by using disc diffusion method. Three bacteria and two fungi have been used to assay the extract for antimicrobial activity and the result has been recorded. Consequently, the extract was analyzed using HPLC chromatogram to detect the production of major and minor metabolites for different media, different strength and different incubation period. All the data and information obtained from the observations, weight of extracts, microscopic pictures, antimicrobial test and HPLC chromatograms analysis were collected and recorded. 2008 Thesis https://ir.uitm.edu.my/id/eprint/101094/ https://ir.uitm.edu.my/id/eprint/101094/1/101094.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Pharmaceutical chemistry
spellingShingle	Pharmaceutical chemistry Mohd Abidi, Nor Azizah Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi
description	The study was done to identify the effect of culture condition on metabolite production by fungal strain 4PL2. fungi strain 4PL2 was isolated from plant of Pandanus herbaceous which was collected from Port Dickson, Negeri Sembilan in 2006. the study was performed by growing the pure culture of fungi strain 4PL2 in different media, different strength and different incubation period. The pure culture of 4PL2 was then incubated for 2 weeks, 3 weeks and 4 weeks. Then the myceliums of fungi strain 4PL2 were subjected to extraction process by using ethyl acetate as a solvent. After obtaining the pure extracts, then subjected to antimicrobial assay by using disc diffusion method. Three bacteria and two fungi have been used to assay the extract for antimicrobial activity and the result has been recorded. Consequently, the extract was analyzed using HPLC chromatogram to detect the production of major and minor metabolites for different media, different strength and different incubation period. All the data and information obtained from the observations, weight of extracts, microscopic pictures, antimicrobial test and HPLC chromatograms analysis were collected and recorded.
format	Thesis
qualification_level	Bachelor degree
author	Mohd Abidi, Nor Azizah
author_facet	Mohd Abidi, Nor Azizah
author_sort	Mohd Abidi, Nor Azizah
title	Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi
title_short	Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi
title_full	Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi
title_fullStr	Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi
title_full_unstemmed	Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi
title_sort	effect of culture condition on metabolite using osmac approach produced by endophytic strain (4pl2) / nor azizah mohd abidi
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2008
url	https://ir.uitm.edu.my/id/eprint/101094/1/101094.PDF
_version_	1811769129989832704

Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi

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