Determination of interferon-γ level in peripheral blood mononuclear cells treated with Gynura procumbens ethanolic extract / Hanalene Deliza Husaini

Interferon-gamma is a type of cytokine that is responsible for mediating the immune response by increasing the activity macrophages, controlling the inflammatory response and more. However, excessive production of interferon-gamma would potentially lead to autoimmune diseases. Gynura procumbens, a t...

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Main Author:	Husaini, Hanalene Deliza
Format:	Thesis
Language:	English
Published:	2016
Online Access:	https://ir.uitm.edu.my/id/eprint/101098/1/101098.pdf
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id	my-uitm-ir.101098
record_format	uketd_dc
spelling	my-uitm-ir.1010982024-08-27T17:13:25Z Determination of interferon-γ level in peripheral blood mononuclear cells treated with Gynura procumbens ethanolic extract / Hanalene Deliza Husaini 2016 Husaini, Hanalene Deliza Interferon-gamma is a type of cytokine that is responsible for mediating the immune response by increasing the activity macrophages, controlling the inflammatory response and more. However, excessive production of interferon-gamma would potentially lead to autoimmune diseases. Gynura procumbens, a type of herb has been used by the locals for its wide range of therapeutic properties against diseases. Gynura procumbens have shown to activate the proliferation T helper lymphocytes which are one of the main producers of interferon-gamma. The aim of this study is to evaluate the immunomodulatory properties of Gynura procumbens by measuring the level of interferon-γ in peripheral blood mononuclear cell (PBMC). Supernatant of PBMC cultures treated with Gynura procumbens ethanolic extract were aspirated and analysed using Luminex® assay. Results demonstrated no exact value (<49.259 ρg/ml) for all test because the concentration was too low for the detection range of the kit. This may indicate that Gynura procumbens did not stimulate the production of interferon-gamma, thus did not affect the immune system. This, in turn, leads to the fact that Gynura procumbens is safe to be consumed without toxic effects. This study should be repeated with a broader standard range of interferon-gamma in order to obtain an exact value of the concentration of interferon-gamma. 2016 Thesis https://ir.uitm.edu.my/id/eprint/101098/ https://ir.uitm.edu.my/id/eprint/101098/1/101098.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Health Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
description	Interferon-gamma is a type of cytokine that is responsible for mediating the immune response by increasing the activity macrophages, controlling the inflammatory response and more. However, excessive production of interferon-gamma would potentially lead to autoimmune diseases. Gynura procumbens, a type of herb has been used by the locals for its wide range of therapeutic properties against diseases. Gynura procumbens have shown to activate the proliferation T helper lymphocytes which are one of the main producers of interferon-gamma. The aim of this study is to evaluate the immunomodulatory properties of Gynura procumbens by measuring the level of interferon-γ in peripheral blood mononuclear cell (PBMC). Supernatant of PBMC cultures treated with Gynura procumbens ethanolic extract were aspirated and analysed using Luminex® assay. Results demonstrated no exact value (<49.259 ρg/ml) for all test because the concentration was too low for the detection range of the kit. This may indicate that Gynura procumbens did not stimulate the production of interferon-gamma, thus did not affect the immune system. This, in turn, leads to the fact that Gynura procumbens is safe to be consumed without toxic effects. This study should be repeated with a broader standard range of interferon-gamma in order to obtain an exact value of the concentration of interferon-gamma.
format	Thesis
qualification_level	Bachelor degree
author	Husaini, Hanalene Deliza
spellingShingle	Husaini, Hanalene Deliza Determination of interferon-γ level in peripheral blood mononuclear cells treated with Gynura procumbens ethanolic extract / Hanalene Deliza Husaini
author_facet	Husaini, Hanalene Deliza
author_sort	Husaini, Hanalene Deliza
title	Determination of interferon-γ level in peripheral blood mononuclear cells treated with Gynura procumbens ethanolic extract / Hanalene Deliza Husaini
title_short	Determination of interferon-γ level in peripheral blood mononuclear cells treated with Gynura procumbens ethanolic extract / Hanalene Deliza Husaini
title_full	Determination of interferon-γ level in peripheral blood mononuclear cells treated with Gynura procumbens ethanolic extract / Hanalene Deliza Husaini
title_fullStr	Determination of interferon-γ level in peripheral blood mononuclear cells treated with Gynura procumbens ethanolic extract / Hanalene Deliza Husaini
title_full_unstemmed	Determination of interferon-γ level in peripheral blood mononuclear cells treated with Gynura procumbens ethanolic extract / Hanalene Deliza Husaini
title_sort	determination of interferon-γ level in peripheral blood mononuclear cells treated with gynura procumbens ethanolic extract / hanalene deliza husaini
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Health Sciences
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/101098/1/101098.pdf
_version_	1811769130240442368

Determination of interferon-γ level in peripheral blood mononuclear cells treated with Gynura procumbens ethanolic extract / Hanalene Deliza Husaini

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