Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin

Gynura procumbens is plant come from family of Asteraceae and is an annual evergreen shrub that grows plenteous in Southest Asia area such as Malaysia, Thailand and Indonesia. G. procumbens is traditionally used for treatment of kidney disease, cancer, rash, constipation, migrains, hypertension, eru...

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Main Author:	Kamaludin, Mohammad Syazwan
Format:	Thesis
Language:	English
Published:	2016
Online Access:	https://ir.uitm.edu.my/id/eprint/101137/1/101137.pdf
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id	my-uitm-ir.101137
record_format	uketd_dc
spelling	my-uitm-ir.1011372024-08-27T17:13:09Z Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin 2016 Kamaludin, Mohammad Syazwan Gynura procumbens is plant come from family of Asteraceae and is an annual evergreen shrub that grows plenteous in Southest Asia area such as Malaysia, Thailand and Indonesia. G. procumbens is traditionally used for treatment of kidney disease, cancer, rash, constipation, migrains, hypertension, eruptive fevers and diabetes mellitus. The following project was conducted to study the determination of Tumor Necrosis Factor – α (TNF – α) level in Peripheral Blood Mononuclear Cells (PBMC) when treated by using G.procumbens. G.procumbens leaf was extracted by aqueous and ethanolic method with different concentration (50 μg/mL, 200 μg/mL and 800 μg/mL). Fresh human blood then layered with Ficoll using density gradient separation segregation method to separate the red blood cells and white blood cells. Then at cell culture method, G.procumbens ethanolic and aqueous extraction and PBMC will be added to different comparing group. RPMI used as a media, Polymyxin B as a LPS inhibitor and CLI 0.95 acts as TLR4 inhibitor. The level detection of TNF – α was determined by using Luminex® Magnetic Assay. Even though, comparing with aqueous and ethanolic extraction with different concentration (50 μg/mL, 200 μg/mL and 800 μg/mL), there was no exact value in the expression of TNF – α when detection by using Luminex® Magnetic Assay. For that reason, statistical analysis by using SPSS method was unnecessary to be calculated. The result proved that G.procumbens is safe to be consumed by humans because G.procumbens does not show effect on the production of TNF – α because it is the one of the pro-inflammatory cytokines which is important in signaling and promote systemic inflammation. ). In contrast, G.procumbens exhibit in function as TNF – α inhibitor while TNF – α is vice versa. 2016 Thesis https://ir.uitm.edu.my/id/eprint/101137/ https://ir.uitm.edu.my/id/eprint/101137/1/101137.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Health Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
description	Gynura procumbens is plant come from family of Asteraceae and is an annual evergreen shrub that grows plenteous in Southest Asia area such as Malaysia, Thailand and Indonesia. G. procumbens is traditionally used for treatment of kidney disease, cancer, rash, constipation, migrains, hypertension, eruptive fevers and diabetes mellitus. The following project was conducted to study the determination of Tumor Necrosis Factor – α (TNF – α) level in Peripheral Blood Mononuclear Cells (PBMC) when treated by using G.procumbens. G.procumbens leaf was extracted by aqueous and ethanolic method with different concentration (50 μg/mL, 200 μg/mL and 800 μg/mL). Fresh human blood then layered with Ficoll using density gradient separation segregation method to separate the red blood cells and white blood cells. Then at cell culture method, G.procumbens ethanolic and aqueous extraction and PBMC will be added to different comparing group. RPMI used as a media, Polymyxin B as a LPS inhibitor and CLI 0.95 acts as TLR4 inhibitor. The level detection of TNF – α was determined by using Luminex® Magnetic Assay. Even though, comparing with aqueous and ethanolic extraction with different concentration (50 μg/mL, 200 μg/mL and 800 μg/mL), there was no exact value in the expression of TNF – α when detection by using Luminex® Magnetic Assay. For that reason, statistical analysis by using SPSS method was unnecessary to be calculated. The result proved that G.procumbens is safe to be consumed by humans because G.procumbens does not show effect on the production of TNF – α because it is the one of the pro-inflammatory cytokines which is important in signaling and promote systemic inflammation. ). In contrast, G.procumbens exhibit in function as TNF – α inhibitor while TNF – α is vice versa.
format	Thesis
qualification_level	Bachelor degree
author	Kamaludin, Mohammad Syazwan
spellingShingle	Kamaludin, Mohammad Syazwan Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin
author_facet	Kamaludin, Mohammad Syazwan
author_sort	Kamaludin, Mohammad Syazwan
title	Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin
title_short	Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin
title_full	Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin
title_fullStr	Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin
title_full_unstemmed	Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin
title_sort	determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / mohammad syazwan kamaludin
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Health Sciences
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/101137/1/101137.pdf
_version_	1811769134022656000

Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin

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