Determination of il-10 expression in peripheral blood mononuclear cells (PBMCs) treated with ethanolic extract of Gynura procumbens / Muhammad Azim Aizuddin Shokhimi

Determination of il-10 expression in peripheral blood mononuclear cells (PBMCs) treated with ethanolic extract of Gynura procumbens / Muhammad Azim Aizuddin Shokhimi

Gynura procumbens (G. procumbens) is a traditional medicinal herb that commonly used in South-East Asia as food consumption to treat inflammation, skin rashes and viral infection. Interleukin-10 (IL-10) is an anti-inflammatory cytokine act to activate macrophages and B cells, inhibit mast cells and...

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Main Author: Shokhimi, Muhammad Azim Aizuddin
Format: Thesis
Language:English
Published: 2016
Online Access:https://ir.uitm.edu.my/id/eprint/101212/1/101212.pdf
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spelling my-uitm-ir.1012122024-08-27T17:21:57Z Determination of il-10 expression in peripheral blood mononuclear cells (PBMCs) treated with ethanolic extract of Gynura procumbens / Muhammad Azim Aizuddin Shokhimi 2016 Shokhimi, Muhammad Azim Aizuddin Gynura procumbens (G. procumbens) is a traditional medicinal herb that commonly used in South-East Asia as food consumption to treat inflammation, skin rashes and viral infection. Interleukin-10 (IL-10) is an anti-inflammatory cytokine act to activate macrophages and B cells, inhibit mast cells and T-helper 1 (Th1) cells, and stimulate T-helper 2 (Th2) cells. This cytokine activated from monocytes, macrophages, and different T-cell subsets which can be mainly found in peripheral blood mononuclear cells (PBMCs). Our aim was to determine the expression of IL-10 in PBMCs treated with G. Procumbens aqueous extraction. Peripheral Blood Mononuclear Cells (PBMCs) isolated from whole blood by using Ficoll gradient density were used to determine the activities of G. Procumbens on lymphocytes, monocytes and macrophages function to induce the expression of inflammatory cytokines, IL-10 with different concentrations of G. Procumbens aqueous extraction (50, 200 and 800 µg/ml). Viability of PBMCs was observed and calculated after staining with Trypan blue and Wright stain. A specific Toll-like receptor (TLR) 4 inhibitor, CLI095 was used to determine whether or not G. Procumbens exerts its effects through TLR4. An antagonist of lipopolysaccharides (LPS), polymyxin B, was used to evaluate whether endotoxin properties in G. Procumbens contributed to its immunomodulatory activity. Cell culture supernatants were analysed by using Luminex® Human Screening Magnetic Assay. G. procumbens exhibited not significantly potent immunomodulatory activity of IL-10 by PBMCs. The expression of IL-10 were increased upon exposure to different concentrations of G. procumbens by co-treatment with CLI-095 (TLR4 inhibitor) but partially decreased by polymyxin B (LPS-neutralizer). One-way ANOVA of those treatment groups compared to control group give p>0.05 followed by post-hoc multiple comparison Dunnett’s test. We demonstrate impotent immunomodulatory activity of G. procumbens towards the expression of IL-10 by PBMCs. We believe that this immunomodulatory activity is due to endotoxin-like properties of G. procumbens via TLR4 receptor and MyD88 or Toll/IL-1R domain-containing adaptor inducing IFN-β (TRIF) - signalling pathways. 2016 Thesis https://ir.uitm.edu.my/id/eprint/101212/ https://ir.uitm.edu.my/id/eprint/101212/1/101212.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Health Sciences
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
description Gynura procumbens (G. procumbens) is a traditional medicinal herb that commonly used in South-East Asia as food consumption to treat inflammation, skin rashes and viral infection. Interleukin-10 (IL-10) is an anti-inflammatory cytokine act to activate macrophages and B cells, inhibit mast cells and T-helper 1 (Th1) cells, and stimulate T-helper 2 (Th2) cells. This cytokine activated from monocytes, macrophages, and different T-cell subsets which can be mainly found in peripheral blood mononuclear cells (PBMCs). Our aim was to determine the expression of IL-10 in PBMCs treated with G. Procumbens aqueous extraction. Peripheral Blood Mononuclear Cells (PBMCs) isolated from whole blood by using Ficoll gradient density were used to determine the activities of G. Procumbens on lymphocytes, monocytes and macrophages function to induce the expression of inflammatory cytokines, IL-10 with different concentrations of G. Procumbens aqueous extraction (50, 200 and 800 µg/ml). Viability of PBMCs was observed and calculated after staining with Trypan blue and Wright stain. A specific Toll-like receptor (TLR) 4 inhibitor, CLI095 was used to determine whether or not G. Procumbens exerts its effects through TLR4. An antagonist of lipopolysaccharides (LPS), polymyxin B, was used to evaluate whether endotoxin properties in G. Procumbens contributed to its immunomodulatory activity. Cell culture supernatants were analysed by using Luminex® Human Screening Magnetic Assay. G. procumbens exhibited not significantly potent immunomodulatory activity of IL-10 by PBMCs. The expression of IL-10 were increased upon exposure to different concentrations of G. procumbens by co-treatment with CLI-095 (TLR4 inhibitor) but partially decreased by polymyxin B (LPS-neutralizer). One-way ANOVA of those treatment groups compared to control group give p>0.05 followed by post-hoc multiple comparison Dunnett’s test. We demonstrate impotent immunomodulatory activity of G. procumbens towards the expression of IL-10 by PBMCs. We believe that this immunomodulatory activity is due to endotoxin-like properties of G. procumbens via TLR4 receptor and MyD88 or Toll/IL-1R domain-containing adaptor inducing IFN-β (TRIF) - signalling pathways.
format Thesis
qualification_level Bachelor degree
author Shokhimi, Muhammad Azim Aizuddin
spellingShingle Shokhimi, Muhammad Azim Aizuddin
Determination of il-10 expression in peripheral blood mononuclear cells (PBMCs) treated with ethanolic extract of Gynura procumbens / Muhammad Azim Aizuddin Shokhimi
author_facet Shokhimi, Muhammad Azim Aizuddin
author_sort Shokhimi, Muhammad Azim Aizuddin
title Determination of il-10 expression in peripheral blood mononuclear cells (PBMCs) treated with ethanolic extract of Gynura procumbens / Muhammad Azim Aizuddin Shokhimi
title_short Determination of il-10 expression in peripheral blood mononuclear cells (PBMCs) treated with ethanolic extract of Gynura procumbens / Muhammad Azim Aizuddin Shokhimi
title_full Determination of il-10 expression in peripheral blood mononuclear cells (PBMCs) treated with ethanolic extract of Gynura procumbens / Muhammad Azim Aizuddin Shokhimi
title_fullStr Determination of il-10 expression in peripheral blood mononuclear cells (PBMCs) treated with ethanolic extract of Gynura procumbens / Muhammad Azim Aizuddin Shokhimi
title_full_unstemmed Determination of il-10 expression in peripheral blood mononuclear cells (PBMCs) treated with ethanolic extract of Gynura procumbens / Muhammad Azim Aizuddin Shokhimi
title_sort determination of il-10 expression in peripheral blood mononuclear cells (pbmcs) treated with ethanolic extract of gynura procumbens / muhammad azim aizuddin shokhimi
granting_institution Universiti Teknologi MARA (UiTM)
granting_department Faculty of Health Sciences
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/101212/1/101212.pdf
_version_ 1811769146795360256

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