Isolation of hemicellulose from rice straw by alkaline extraction / Athirah Zawani Amran

An alkaline extraction method was developed for the extraction of two types of hemicellulose; hemicellulose with lignin (HL) and hemicellulose without lignin (HWL) from rice straw. The extraction of hemicellulose was done using sodium hydroxide (NaOH). Hemicellulose is an alkaline soluble compound;...

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Main Author: Amran, Athirah Zawani
Format: Thesis
Language:English
Published: 2010
Online Access:https://ir.uitm.edu.my/id/eprint/101250/1/101250.pdf
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spelling my-uitm-ir.1012502024-08-30T15:59:44Z Isolation of hemicellulose from rice straw by alkaline extraction / Athirah Zawani Amran 2010 Amran, Athirah Zawani An alkaline extraction method was developed for the extraction of two types of hemicellulose; hemicellulose with lignin (HL) and hemicellulose without lignin (HWL) from rice straw. The extraction of hemicellulose was done using sodium hydroxide (NaOH). Hemicellulose is an alkaline soluble compound; therefore extraction using NaOH is the most suitable method to isolate hemicellulose especially from non-woody plants such as rice straw. Several factors such as NaOH concentration, temperature and pH were optimized for maximum production of these hemicelluloses. Rice straw was mixed with NaOH at concentration of 1 % - 5 %, temperature of 45 °C - 65 °C and at pH of 4.5 - 6.5. The determination of hemicellulose concentrations were done using UV - Visible Spectrophotometer and the hemicellulose concentrations were analyzed by determining the total sugar. The results showed that the optimum condition for the extraction of HL was at 1 % of NaOH, 65 °C and pH of 5.5 while for the extraction of HWL, the optimum conditions was at 3 % NaOH, 45 °C and pH of 5.5. The concentration for HL determined using UV - Visible Spectrophotometer at 1 % NaOH, temperature of 65 °C and pH 5.5 were 7.44 ug/mL, 18.01 ug/mL and 22.99 ug/mL, respectively. On the other hand, the HWL concentration at optimum NaOH concentration, temperature and pH were 32.06 ug/mL, 63.56 ug/mL and 33.77 ug/mL, respectively. 2010 Thesis https://ir.uitm.edu.my/id/eprint/101250/ https://ir.uitm.edu.my/id/eprint/101250/1/101250.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Applied Science
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
description An alkaline extraction method was developed for the extraction of two types of hemicellulose; hemicellulose with lignin (HL) and hemicellulose without lignin (HWL) from rice straw. The extraction of hemicellulose was done using sodium hydroxide (NaOH). Hemicellulose is an alkaline soluble compound; therefore extraction using NaOH is the most suitable method to isolate hemicellulose especially from non-woody plants such as rice straw. Several factors such as NaOH concentration, temperature and pH were optimized for maximum production of these hemicelluloses. Rice straw was mixed with NaOH at concentration of 1 % - 5 %, temperature of 45 °C - 65 °C and at pH of 4.5 - 6.5. The determination of hemicellulose concentrations were done using UV - Visible Spectrophotometer and the hemicellulose concentrations were analyzed by determining the total sugar. The results showed that the optimum condition for the extraction of HL was at 1 % of NaOH, 65 °C and pH of 5.5 while for the extraction of HWL, the optimum conditions was at 3 % NaOH, 45 °C and pH of 5.5. The concentration for HL determined using UV - Visible Spectrophotometer at 1 % NaOH, temperature of 65 °C and pH 5.5 were 7.44 ug/mL, 18.01 ug/mL and 22.99 ug/mL, respectively. On the other hand, the HWL concentration at optimum NaOH concentration, temperature and pH were 32.06 ug/mL, 63.56 ug/mL and 33.77 ug/mL, respectively.
format Thesis
qualification_level Bachelor degree
author Amran, Athirah Zawani
spellingShingle Amran, Athirah Zawani
Isolation of hemicellulose from rice straw by alkaline extraction / Athirah Zawani Amran
author_facet Amran, Athirah Zawani
author_sort Amran, Athirah Zawani
title Isolation of hemicellulose from rice straw by alkaline extraction / Athirah Zawani Amran
title_short Isolation of hemicellulose from rice straw by alkaline extraction / Athirah Zawani Amran
title_full Isolation of hemicellulose from rice straw by alkaline extraction / Athirah Zawani Amran
title_fullStr Isolation of hemicellulose from rice straw by alkaline extraction / Athirah Zawani Amran
title_full_unstemmed Isolation of hemicellulose from rice straw by alkaline extraction / Athirah Zawani Amran
title_sort isolation of hemicellulose from rice straw by alkaline extraction / athirah zawani amran
granting_institution Universiti Teknologi MARA (UiTM)
granting_department Faculty of Applied Science
publishDate 2010
url https://ir.uitm.edu.my/id/eprint/101250/1/101250.pdf
_version_ 1811769151497175040