Qualitative phytochemical analysis and antimicrobial activity of piper sarmentosum leaves extract against wound pathogens / Nur Aziatul Syakila Mohamad Radzi

Piper sarmentosum also known as “Daun Kaduk” is one of the plants that are used as a traditional medicine that usually can be found in the tropical and subtropical region of the world. Local people also used this plant to cure skin disease, rheumatism, diarrhea, fever and headache. Previous studies...

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Main Author:	Mohamad Radzi, Nur Aziatul Syakila
Format:	Thesis
Language:	English
Published:	2016
Online Access:	https://ir.uitm.edu.my/id/eprint/101410/1/101410.pdf
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spelling	my-uitm-ir.1014102024-09-12T16:08:59Z Qualitative phytochemical analysis and antimicrobial activity of piper sarmentosum leaves extract against wound pathogens / Nur Aziatul Syakila Mohamad Radzi 2016 Mohamad Radzi, Nur Aziatul Syakila Piper sarmentosum also known as “Daun Kaduk” is one of the plants that are used as a traditional medicine that usually can be found in the tropical and subtropical region of the world. Local people also used this plant to cure skin disease, rheumatism, diarrhea, fever and headache. Previous studies showed that Piper sarmentosum extract can act as antimicrobial agents. Bioactive constituents in antiseptic are reported to be cytotoxic to human cell. Thus, it is important to search for new types of highly effective and non-toxic antimicrobial agents from natural sources. The aim of this study is to investigate the phytochemical compounds and antimicrobial activity of Piper sarmentosum leaves extract against wound pathogens. In order to determine the antimicrobial activity of Piper sarmentosum extract against wound pathogens, antimicrobial susceptibility testing (AST) by disc diffusion method and minimum inhibitory concentration (MIC) by using microdilution broth method was performed. The phytochemical compounds were screened using standardized methods and the results were interpreted based on precipitation and color changes of the chemical reagents. The present study demonstrated the antimicrobial activity of methanolic extract of Piper sarmentosum leaves. The result showed that antimicrobial activity was observed against S.aureus (7mm), while the extract showed little effect against E.coli (6.5mm). There is no zone of inhibition against P.aeruginosa. The result for MIC value showed the minimum concentration of Piper sarmentosum extract to inhibit the S.aureus and E.coli (6.25mg/ml and 12.5mg/ml). In addition, the phytochemical screening result showed that glycosides, flavonoids, terpenoids, alkaloids, and phenolics were present in the methanolic extract. In conclusion, methanolic extract of Piper sarmentosum could be a possible source to obtain new and effective herbal medicines to treat wound infections caused by multi-drug resistant strains of microorganisms from community as well as hospital settings. 2016 Thesis https://ir.uitm.edu.my/id/eprint/101410/ https://ir.uitm.edu.my/id/eprint/101410/1/101410.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Health Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
description	Piper sarmentosum also known as “Daun Kaduk” is one of the plants that are used as a traditional medicine that usually can be found in the tropical and subtropical region of the world. Local people also used this plant to cure skin disease, rheumatism, diarrhea, fever and headache. Previous studies showed that Piper sarmentosum extract can act as antimicrobial agents. Bioactive constituents in antiseptic are reported to be cytotoxic to human cell. Thus, it is important to search for new types of highly effective and non-toxic antimicrobial agents from natural sources. The aim of this study is to investigate the phytochemical compounds and antimicrobial activity of Piper sarmentosum leaves extract against wound pathogens. In order to determine the antimicrobial activity of Piper sarmentosum extract against wound pathogens, antimicrobial susceptibility testing (AST) by disc diffusion method and minimum inhibitory concentration (MIC) by using microdilution broth method was performed. The phytochemical compounds were screened using standardized methods and the results were interpreted based on precipitation and color changes of the chemical reagents. The present study demonstrated the antimicrobial activity of methanolic extract of Piper sarmentosum leaves. The result showed that antimicrobial activity was observed against S.aureus (7mm), while the extract showed little effect against E.coli (6.5mm). There is no zone of inhibition against P.aeruginosa. The result for MIC value showed the minimum concentration of Piper sarmentosum extract to inhibit the S.aureus and E.coli (6.25mg/ml and 12.5mg/ml). In addition, the phytochemical screening result showed that glycosides, flavonoids, terpenoids, alkaloids, and phenolics were present in the methanolic extract. In conclusion, methanolic extract of Piper sarmentosum could be a possible source to obtain new and effective herbal medicines to treat wound infections caused by multi-drug resistant strains of microorganisms from community as well as hospital settings.
format	Thesis
qualification_level	Bachelor degree
author	Mohamad Radzi, Nur Aziatul Syakila
spellingShingle	Mohamad Radzi, Nur Aziatul Syakila Qualitative phytochemical analysis and antimicrobial activity of piper sarmentosum leaves extract against wound pathogens / Nur Aziatul Syakila Mohamad Radzi
author_facet	Mohamad Radzi, Nur Aziatul Syakila
author_sort	Mohamad Radzi, Nur Aziatul Syakila
title	Qualitative phytochemical analysis and antimicrobial activity of piper sarmentosum leaves extract against wound pathogens / Nur Aziatul Syakila Mohamad Radzi
title_short	Qualitative phytochemical analysis and antimicrobial activity of piper sarmentosum leaves extract against wound pathogens / Nur Aziatul Syakila Mohamad Radzi
title_full	Qualitative phytochemical analysis and antimicrobial activity of piper sarmentosum leaves extract against wound pathogens / Nur Aziatul Syakila Mohamad Radzi
title_fullStr	Qualitative phytochemical analysis and antimicrobial activity of piper sarmentosum leaves extract against wound pathogens / Nur Aziatul Syakila Mohamad Radzi
title_full_unstemmed	Qualitative phytochemical analysis and antimicrobial activity of piper sarmentosum leaves extract against wound pathogens / Nur Aziatul Syakila Mohamad Radzi
title_sort	qualitative phytochemical analysis and antimicrobial activity of piper sarmentosum leaves extract against wound pathogens / nur aziatul syakila mohamad radzi
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Health Sciences
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/101410/1/101410.pdf
_version_	1811769165823868928

Qualitative phytochemical analysis and antimicrobial activity of piper sarmentosum leaves extract against wound pathogens / Nur Aziatul Syakila Mohamad Radzi

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