Evaluation of hexane extract of parkia speciosa hassk. pericarp as a potential antimicrobial agent / Nurul Afiqah Ahmad Zaidi

Parkia speciosa Hassk. or locally named as “Petai” in Malaysia, belongs to the family Fabaceae. It is known to have many medicinal benefits. In the present study, the discarded pericarp of Parkia speciosa was extracted with n-hexane solvent for assessment on its antimicrobial activities. Antimicrobi...

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Main Author: Ahmad Zaidi, Nurul Afiqah
Format: Thesis
Language:English
Published: 2007
Online Access:https://ir.uitm.edu.my/id/eprint/101442/1/101442.pdf
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spelling my-uitm-ir.1014422024-09-12T16:15:20Z Evaluation of hexane extract of parkia speciosa hassk. pericarp as a potential antimicrobial agent / Nurul Afiqah Ahmad Zaidi 2007 Ahmad Zaidi, Nurul Afiqah Parkia speciosa Hassk. or locally named as “Petai” in Malaysia, belongs to the family Fabaceae. It is known to have many medicinal benefits. In the present study, the discarded pericarp of Parkia speciosa was extracted with n-hexane solvent for assessment on its antimicrobial activities. Antimicrobial susceptibility testing (AST) of the Parkia speciosa extract was done using five concentrations of hexane extract by using the disc diffusion method on S. aureus, B. cereus, E. coli, and S. thyphimurium. The zone of inhibition for AST was only shown for gram positive bacteria which were S. aureus and B. cereus whereas for gram negative bacteria (E. coli and S. thyphimurium) showed no zone of inhibition. The minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Parkia speciosa pericarp hexane extract were also determined by micro-titer method against the four tested bacteria. The MIC result for S. aureus and B. cereus at three concentrations of hexane extract which were 1000 mg/ml, 500 mg/ml and 250 mg/ml showed that no turbidity was seen. MBC result of the three concentrations of extract also showed that there was no growth the Muller Hinton agar. In conclusion, even though the hexane extract of Parkia speciosa pericarp showed antimicrobial activity towards S. aureus and B. cereus, it may not be a good candidate as a potential antimicrobial agent due to the limitations during the preparation of the extract. 2007 Thesis https://ir.uitm.edu.my/id/eprint/101442/ https://ir.uitm.edu.my/id/eprint/101442/1/101442.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
description Parkia speciosa Hassk. or locally named as “Petai” in Malaysia, belongs to the family Fabaceae. It is known to have many medicinal benefits. In the present study, the discarded pericarp of Parkia speciosa was extracted with n-hexane solvent for assessment on its antimicrobial activities. Antimicrobial susceptibility testing (AST) of the Parkia speciosa extract was done using five concentrations of hexane extract by using the disc diffusion method on S. aureus, B. cereus, E. coli, and S. thyphimurium. The zone of inhibition for AST was only shown for gram positive bacteria which were S. aureus and B. cereus whereas for gram negative bacteria (E. coli and S. thyphimurium) showed no zone of inhibition. The minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Parkia speciosa pericarp hexane extract were also determined by micro-titer method against the four tested bacteria. The MIC result for S. aureus and B. cereus at three concentrations of hexane extract which were 1000 mg/ml, 500 mg/ml and 250 mg/ml showed that no turbidity was seen. MBC result of the three concentrations of extract also showed that there was no growth the Muller Hinton agar. In conclusion, even though the hexane extract of Parkia speciosa pericarp showed antimicrobial activity towards S. aureus and B. cereus, it may not be a good candidate as a potential antimicrobial agent due to the limitations during the preparation of the extract.
format Thesis
qualification_level Bachelor degree
author Ahmad Zaidi, Nurul Afiqah
spellingShingle Ahmad Zaidi, Nurul Afiqah
Evaluation of hexane extract of parkia speciosa hassk. pericarp as a potential antimicrobial agent / Nurul Afiqah Ahmad Zaidi
author_facet Ahmad Zaidi, Nurul Afiqah
author_sort Ahmad Zaidi, Nurul Afiqah
title Evaluation of hexane extract of parkia speciosa hassk. pericarp as a potential antimicrobial agent / Nurul Afiqah Ahmad Zaidi
title_short Evaluation of hexane extract of parkia speciosa hassk. pericarp as a potential antimicrobial agent / Nurul Afiqah Ahmad Zaidi
title_full Evaluation of hexane extract of parkia speciosa hassk. pericarp as a potential antimicrobial agent / Nurul Afiqah Ahmad Zaidi
title_fullStr Evaluation of hexane extract of parkia speciosa hassk. pericarp as a potential antimicrobial agent / Nurul Afiqah Ahmad Zaidi
title_full_unstemmed Evaluation of hexane extract of parkia speciosa hassk. pericarp as a potential antimicrobial agent / Nurul Afiqah Ahmad Zaidi
title_sort evaluation of hexane extract of parkia speciosa hassk. pericarp as a potential antimicrobial agent / nurul afiqah ahmad zaidi
granting_institution Universiti Teknologi MARA (UiTM)
granting_department Faculty of Applied Sciences
publishDate 2007
url https://ir.uitm.edu.my/id/eprint/101442/1/101442.pdf
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