Molecular epidemiology of giardia duodenalis isolates from sika deer and javan rusa in Sungai Lembing, Pahang / Nurul Azmiera Zamri

Giardia duodenalis is a binucleate intestinal parasite that has two forms of life cycle which are trophozoite and cyst. Cyst is the infective stage while trophozoite attaches itself to our intestinal villi. It can be divided into eight different genetic assemblages starting from A to H. Common assem...

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Main Author:	Zamri, Nurul Azmiera
Format:	Thesis
Language:	English
Published:	2016
Online Access:	https://ir.uitm.edu.my/id/eprint/101444/1/101444.pdf
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id	my-uitm-ir.101444
record_format	uketd_dc
spelling	my-uitm-ir.1014442024-09-12T16:12:04Z Molecular epidemiology of giardia duodenalis isolates from sika deer and javan rusa in Sungai Lembing, Pahang / Nurul Azmiera Zamri 2016 Zamri, Nurul Azmiera Giardia duodenalis is a binucleate intestinal parasite that has two forms of life cycle which are trophozoite and cyst. Cyst is the infective stage while trophozoite attaches itself to our intestinal villi. It can be divided into eight different genetic assemblages starting from A to H. Common assemblages infecting the human which is A and B, found in the animals, open a recent transmission mode called zoonotic. Giardiasis is a common disease associated with G. duodenalis and gives common clinical symptoms such as diarrhea, malabsorption and abdominal pain. Recently, prevalence studies for G. duodenalis in Malaysia are starting to focus on farm animals. However, there is a limited data on the characterization of G. duodenalis from deer farm in this country. Therefore, this research is conducted to determine the prevalence of G. duodenalis from Sika deer and Javan rusa deer in Sungai Lembing, Pahang. A cross-sectional study was conducted in D’Paradise deer farm, one of the largest deer farm in Malaysia, located in Sungai Lembing, Pahang. A total 50 stool samples were collected, 25 samples each from two species of deer involved in this research which are Sika deer (Cervus nippon) and Javan rusa (Cervus timorensis). The stool samples collected were analysed microscopically using Wheatley’s trichrome staining technique and nested polymerase chain reaction by the amplification of triosephosphate isomerase gene. Both methods showed prevalence of 0% (0/50) for G. duodenalis in both species. Therefore, more studies with a broad range of deer farms and various species of deer could be performed to further understand the characterization of G. duodenalis from deer in Malaysia. 2016 Thesis https://ir.uitm.edu.my/id/eprint/101444/ https://ir.uitm.edu.my/id/eprint/101444/1/101444.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Health Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
description	Giardia duodenalis is a binucleate intestinal parasite that has two forms of life cycle which are trophozoite and cyst. Cyst is the infective stage while trophozoite attaches itself to our intestinal villi. It can be divided into eight different genetic assemblages starting from A to H. Common assemblages infecting the human which is A and B, found in the animals, open a recent transmission mode called zoonotic. Giardiasis is a common disease associated with G. duodenalis and gives common clinical symptoms such as diarrhea, malabsorption and abdominal pain. Recently, prevalence studies for G. duodenalis in Malaysia are starting to focus on farm animals. However, there is a limited data on the characterization of G. duodenalis from deer farm in this country. Therefore, this research is conducted to determine the prevalence of G. duodenalis from Sika deer and Javan rusa deer in Sungai Lembing, Pahang. A cross-sectional study was conducted in D’Paradise deer farm, one of the largest deer farm in Malaysia, located in Sungai Lembing, Pahang. A total 50 stool samples were collected, 25 samples each from two species of deer involved in this research which are Sika deer (Cervus nippon) and Javan rusa (Cervus timorensis). The stool samples collected were analysed microscopically using Wheatley’s trichrome staining technique and nested polymerase chain reaction by the amplification of triosephosphate isomerase gene. Both methods showed prevalence of 0% (0/50) for G. duodenalis in both species. Therefore, more studies with a broad range of deer farms and various species of deer could be performed to further understand the characterization of G. duodenalis from deer in Malaysia.
format	Thesis
qualification_level	Bachelor degree
author	Zamri, Nurul Azmiera
spellingShingle	Zamri, Nurul Azmiera Molecular epidemiology of giardia duodenalis isolates from sika deer and javan rusa in Sungai Lembing, Pahang / Nurul Azmiera Zamri
author_facet	Zamri, Nurul Azmiera
author_sort	Zamri, Nurul Azmiera
title	Molecular epidemiology of giardia duodenalis isolates from sika deer and javan rusa in Sungai Lembing, Pahang / Nurul Azmiera Zamri
title_short	Molecular epidemiology of giardia duodenalis isolates from sika deer and javan rusa in Sungai Lembing, Pahang / Nurul Azmiera Zamri
title_full	Molecular epidemiology of giardia duodenalis isolates from sika deer and javan rusa in Sungai Lembing, Pahang / Nurul Azmiera Zamri
title_fullStr	Molecular epidemiology of giardia duodenalis isolates from sika deer and javan rusa in Sungai Lembing, Pahang / Nurul Azmiera Zamri
title_full_unstemmed	Molecular epidemiology of giardia duodenalis isolates from sika deer and javan rusa in Sungai Lembing, Pahang / Nurul Azmiera Zamri
title_sort	molecular epidemiology of giardia duodenalis isolates from sika deer and javan rusa in sungai lembing, pahang / nurul azmiera zamri
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Health Sciences
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/101444/1/101444.pdf
_version_	1811769169694162944

Molecular epidemiology of giardia duodenalis isolates from sika deer and javan rusa in Sungai Lembing, Pahang / Nurul Azmiera Zamri

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