The role of dioscorea hispida in inducing DNA damage on placental tissues of maternal rats: a molecular basis / Tengku Aideed Syah Tengku Abu Bakar

Dioscorea hispida (D. hispida) which traditionally known as ‘Ubi Gadong’ is a common food-source among Asian countries’ suburbs. However, the presence of chemically active compound knowingly cyanide in D. hispida is attributable to its potential effects on cellular homeostasis; which may be induced...

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Main Author:	Tengku Abu Bakar, Tengku Aideed Syah
Format:	Thesis
Language:	English
Published:	2016
Online Access:	https://ir.uitm.edu.my/id/eprint/101712/1/101712.pdf
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id	my-uitm-ir.101712
record_format	uketd_dc
spelling	my-uitm-ir.1017122024-09-12T16:11:41Z The role of dioscorea hispida in inducing DNA damage on placental tissues of maternal rats: a molecular basis / Tengku Aideed Syah Tengku Abu Bakar 2016 Tengku Abu Bakar, Tengku Aideed Syah Dioscorea hispida (D. hispida) which traditionally known as ‘Ubi Gadong’ is a common food-source among Asian countries’ suburbs. However, the presence of chemically active compound knowingly cyanide in D. hispida is attributable to its potential effects on cellular homeostasis; which may be induced by excessive free radicals generation-related oxidative stress. This phenomenon warrants for a systematic toxicology analyses. In this animal modelling study, the deleterious effects of D. hispida was assessed by demonstrating molecular DNA damage and oxidative stress in placenta tissues of maternal rats following 15 days of D. hispida’s supplementation by oral gavage. From day 6 until 20th day of gestation, pregnant rats were randomly sorted into non-supplemented or control and supplemented groups. Supplemented groups were classified according to three different concentrations; T1: 250 mg/kg, T2: 500 mg/kg and T3: 1000 mg/kg. On day 21, rats’ placenta tissues were harvested following euthanasia and the detrimental effects were elucidated by using biochemical analysis and comet assay for demonstration of oxidative stress and DNA damage. Comet assay revealed significant increase of DNA damage in rat’s placenta tissues which showed significant different comparison between Cx with T1 and T3 at p value < 0.05. Besides, the results revealed that there is significant different between T3 and T2 with p < 0.05. The ROS level depicted an increasing trend proportional to concentration of D. hispida, however, no significant value was observed between groups. The outcome from present study may postulate that the production of ROS by-products were triggered following D. hispida’s supplementation, and thus highlighted that different of concentration D. hispida supplementation toward maternal rat for 15 days could induces alteration in DNA damage through excessive ROS generation. 2016 Thesis https://ir.uitm.edu.my/id/eprint/101712/ https://ir.uitm.edu.my/id/eprint/101712/1/101712.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Health Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
description	Dioscorea hispida (D. hispida) which traditionally known as ‘Ubi Gadong’ is a common food-source among Asian countries’ suburbs. However, the presence of chemically active compound knowingly cyanide in D. hispida is attributable to its potential effects on cellular homeostasis; which may be induced by excessive free radicals generation-related oxidative stress. This phenomenon warrants for a systematic toxicology analyses. In this animal modelling study, the deleterious effects of D. hispida was assessed by demonstrating molecular DNA damage and oxidative stress in placenta tissues of maternal rats following 15 days of D. hispida’s supplementation by oral gavage. From day 6 until 20th day of gestation, pregnant rats were randomly sorted into non-supplemented or control and supplemented groups. Supplemented groups were classified according to three different concentrations; T1: 250 mg/kg, T2: 500 mg/kg and T3: 1000 mg/kg. On day 21, rats’ placenta tissues were harvested following euthanasia and the detrimental effects were elucidated by using biochemical analysis and comet assay for demonstration of oxidative stress and DNA damage. Comet assay revealed significant increase of DNA damage in rat’s placenta tissues which showed significant different comparison between Cx with T1 and T3 at p value < 0.05. Besides, the results revealed that there is significant different between T3 and T2 with p < 0.05. The ROS level depicted an increasing trend proportional to concentration of D. hispida, however, no significant value was observed between groups. The outcome from present study may postulate that the production of ROS by-products were triggered following D. hispida’s supplementation, and thus highlighted that different of concentration D. hispida supplementation toward maternal rat for 15 days could induces alteration in DNA damage through excessive ROS generation.
format	Thesis
qualification_level	Bachelor degree
author	Tengku Abu Bakar, Tengku Aideed Syah
spellingShingle	Tengku Abu Bakar, Tengku Aideed Syah The role of dioscorea hispida in inducing DNA damage on placental tissues of maternal rats: a molecular basis / Tengku Aideed Syah Tengku Abu Bakar
author_facet	Tengku Abu Bakar, Tengku Aideed Syah
author_sort	Tengku Abu Bakar, Tengku Aideed Syah
title	The role of dioscorea hispida in inducing DNA damage on placental tissues of maternal rats: a molecular basis / Tengku Aideed Syah Tengku Abu Bakar
title_short	The role of dioscorea hispida in inducing DNA damage on placental tissues of maternal rats: a molecular basis / Tengku Aideed Syah Tengku Abu Bakar
title_full	The role of dioscorea hispida in inducing DNA damage on placental tissues of maternal rats: a molecular basis / Tengku Aideed Syah Tengku Abu Bakar
title_fullStr	The role of dioscorea hispida in inducing DNA damage on placental tissues of maternal rats: a molecular basis / Tengku Aideed Syah Tengku Abu Bakar
title_full_unstemmed	The role of dioscorea hispida in inducing DNA damage on placental tissues of maternal rats: a molecular basis / Tengku Aideed Syah Tengku Abu Bakar
title_sort	role of dioscorea hispida in inducing dna damage on placental tissues of maternal rats: a molecular basis / tengku aideed syah tengku abu bakar
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Health Sciences
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/101712/1/101712.pdf
_version_	1811769184381566976

The role of dioscorea hispida in inducing DNA damage on placental tissues of maternal rats: a molecular basis / Tengku Aideed Syah Tengku Abu Bakar

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