Antimicrobial activity of lawsonia inermis extracts against skin pathogens / Umi Hairun Anis Ismail

Resistance antibiotic against microorganism is an emerging issue in health care world nowadays. This creates an urgency for discovery of new antimicrobial agents. The use of medicinal plant as natural source has taken over the research world to overcome the drug resistance infectious diseases. In th...

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Main Author:	Ismail, Umi Hairun Anis
Format:	Thesis
Language:	English
Published:	2016
Online Access:	https://ir.uitm.edu.my/id/eprint/101714/1/101714.pdf
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id	my-uitm-ir.101714
record_format	uketd_dc
spelling	my-uitm-ir.1017142024-09-12T14:53:17Z Antimicrobial activity of lawsonia inermis extracts against skin pathogens / Umi Hairun Anis Ismail 2016 Ismail, Umi Hairun Anis Resistance antibiotic against microorganism is an emerging issue in health care world nowadays. This creates an urgency for discovery of new antimicrobial agents. The use of medicinal plant as natural source has taken over the research world to overcome the drug resistance infectious diseases. In this present study antimicrobial activity of Lawsonia inermis extracts is investigated against bacteria of the skin. Traditional folks of Africa, Asia and Middle East countries ever since have been using this plant in treating skin infections. Four microorganisms including two Gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis) and two Gram-negative bacteria (Proteus mirabilis and Pseudomonas aeruginosa) which are the common bacteria found causing infection treated with aqueous and ethanol extracts of Lawsonia inermis. Antimicrobial activity of the microorganisms is determined by antimicrobial susceptibility testing (AST) by using disc diffusion method and followed by minimum inhibitory concentration (MIC) using broth microdilution method to know the minimum concentration in which the bacteria can be inhibited by the extract. To confirmed MIC, minimum bactericidal concentration (MBC) is established. MBC is determined at the dilution which 99.9% of the organism is dead. Lawsonia inermis extract showed significant antimicrobial activity against both Gram-positive and Gram-negative bacteria. The largest zone of inhibition diameter displayed by S. epidermidis, 16.8 mm (±1.04) in aqueous extract and 23.0 mm (±1.03) in ethanol extract. The minimum value of MIC and MBC for different bacterial strain range from 15.63 µg/ml to 125 µg/ml. in conclusion, the present study proved that Lawsonia inermis can be use as alternative from natural sources to replace the existing antimicrobial agents with less side effects towards the consumers. 2016 Thesis https://ir.uitm.edu.my/id/eprint/101714/ https://ir.uitm.edu.my/id/eprint/101714/1/101714.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Health Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
description	Resistance antibiotic against microorganism is an emerging issue in health care world nowadays. This creates an urgency for discovery of new antimicrobial agents. The use of medicinal plant as natural source has taken over the research world to overcome the drug resistance infectious diseases. In this present study antimicrobial activity of Lawsonia inermis extracts is investigated against bacteria of the skin. Traditional folks of Africa, Asia and Middle East countries ever since have been using this plant in treating skin infections. Four microorganisms including two Gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis) and two Gram-negative bacteria (Proteus mirabilis and Pseudomonas aeruginosa) which are the common bacteria found causing infection treated with aqueous and ethanol extracts of Lawsonia inermis. Antimicrobial activity of the microorganisms is determined by antimicrobial susceptibility testing (AST) by using disc diffusion method and followed by minimum inhibitory concentration (MIC) using broth microdilution method to know the minimum concentration in which the bacteria can be inhibited by the extract. To confirmed MIC, minimum bactericidal concentration (MBC) is established. MBC is determined at the dilution which 99.9% of the organism is dead. Lawsonia inermis extract showed significant antimicrobial activity against both Gram-positive and Gram-negative bacteria. The largest zone of inhibition diameter displayed by S. epidermidis, 16.8 mm (±1.04) in aqueous extract and 23.0 mm (±1.03) in ethanol extract. The minimum value of MIC and MBC for different bacterial strain range from 15.63 µg/ml to 125 µg/ml. in conclusion, the present study proved that Lawsonia inermis can be use as alternative from natural sources to replace the existing antimicrobial agents with less side effects towards the consumers.
format	Thesis
qualification_level	Bachelor degree
author	Ismail, Umi Hairun Anis
spellingShingle	Ismail, Umi Hairun Anis Antimicrobial activity of lawsonia inermis extracts against skin pathogens / Umi Hairun Anis Ismail
author_facet	Ismail, Umi Hairun Anis
author_sort	Ismail, Umi Hairun Anis
title	Antimicrobial activity of lawsonia inermis extracts against skin pathogens / Umi Hairun Anis Ismail
title_short	Antimicrobial activity of lawsonia inermis extracts against skin pathogens / Umi Hairun Anis Ismail
title_full	Antimicrobial activity of lawsonia inermis extracts against skin pathogens / Umi Hairun Anis Ismail
title_fullStr	Antimicrobial activity of lawsonia inermis extracts against skin pathogens / Umi Hairun Anis Ismail
title_full_unstemmed	Antimicrobial activity of lawsonia inermis extracts against skin pathogens / Umi Hairun Anis Ismail
title_sort	antimicrobial activity of lawsonia inermis extracts against skin pathogens / umi hairun anis ismail
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Health Sciences
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/101714/1/101714.pdf
_version_	1811769184885932032

Antimicrobial activity of lawsonia inermis extracts against skin pathogens / Umi Hairun Anis Ismail

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