Determination of IL-10 expression in peripheral blood mononuclear cells (PBMCs) treated with aqueous extract of gynura procumbens / Zuraini Danial

Determination of IL-10 expression in peripheral blood mononuclear cells (PBMCs) treated with aqueous extract of gynura procumbens / Zuraini Danial

Gynura procumbens (G. procumbens) is a traditional medicinal herb that commonly used in South-East Asia as food consumption to treat inflammation, skin rashes and viral infection. Interleukin-10 (IL-10) is an anti-inflammatory cytokine act to activate macrophages and B cells, inhibit mast cells and...

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Main Author: Danial, Zuraini
Format: Thesis
Language:English
Published: 2016
Online Access:https://ir.uitm.edu.my/id/eprint/101717/1/101717.pdf
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spelling my-uitm-ir.1017172024-09-12T14:52:24Z Determination of IL-10 expression in peripheral blood mononuclear cells (PBMCs) treated with aqueous extract of gynura procumbens / Zuraini Danial 2016 Danial, Zuraini Gynura procumbens (G. procumbens) is a traditional medicinal herb that commonly used in South-East Asia as food consumption to treat inflammation, skin rashes and viral infection. Interleukin-10 (IL-10) is an anti-inflammatory cytokine act to activate macrophages and B cells, inhibit mast cells and T-helper 1 (Thl) cells, and stimulate T-helper 2 (Th2) cells. This cytokine activated from monocytes, macrophages, and different T-cell subsets which can be mainly found in peripheral blood mononuclear cells (PBMCs). Our aim was to determine the expression of IL10 in PBMCs treated with G Procumbens aqueous extraction. Peripheral Blood Mononuclear Cells (PBMCs) isolated from whole blood by using Ficoll gradient density were used to determine the activities of G Procumbens on lymphocytes, monocytes and macrophages function to induce the expression of inflammatory cytokines, IL-10 with different concentrations of G Procumbens aqueous extraction (50, 200 and 800 ug/ml). Viability of PBMCs was observed and calculated after staining with Trypan blue and Wright stain. A specific Toll-like receptor (TLR) 4 inhibitor, CLI095 was used to determine whether or not G Procumbens exerts its effects through TLR4. An antagonist of lipopolysaccharides (LPS), polymyxin B, was used to evaluate whether endotoxin properties in G Procumbens contributed to its immunomodulatory activity. Cell culture supernatants were analysed by using Luminex® Human Screening Magnetic Assay. G procumbens exhibited not significantly potent immunomodulatory activity of IL-10 by PBMCs. The expression of IL-10 were increased upon exposure to different concentrations of G procumbens by co-treatment with CLI-095 (TLR4 inhibitor) but partially decreased by polymyxin B (LPS-neutralizer). One-way ANOVA of those treatment groups compared to control group give/?>0.05 followed by post-hoc multiple comparison Dunnett's test. We demonstrate impotent immunomodulatory activity of G procumbens towards the expression of IL-10 by PBMCs. We believe that this immunomodulatory activity is due to endotoxin-like properties of G procumbens via TLR4 receptor and MyD88 or Toll/IL-IR domain-containing adaptor inducing IFN-P (TRIF) - signalling pathways. 2016 Thesis https://ir.uitm.edu.my/id/eprint/101717/ https://ir.uitm.edu.my/id/eprint/101717/1/101717.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Health Sciences
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
description Gynura procumbens (G. procumbens) is a traditional medicinal herb that commonly used in South-East Asia as food consumption to treat inflammation, skin rashes and viral infection. Interleukin-10 (IL-10) is an anti-inflammatory cytokine act to activate macrophages and B cells, inhibit mast cells and T-helper 1 (Thl) cells, and stimulate T-helper 2 (Th2) cells. This cytokine activated from monocytes, macrophages, and different T-cell subsets which can be mainly found in peripheral blood mononuclear cells (PBMCs). Our aim was to determine the expression of IL10 in PBMCs treated with G Procumbens aqueous extraction. Peripheral Blood Mononuclear Cells (PBMCs) isolated from whole blood by using Ficoll gradient density were used to determine the activities of G Procumbens on lymphocytes, monocytes and macrophages function to induce the expression of inflammatory cytokines, IL-10 with different concentrations of G Procumbens aqueous extraction (50, 200 and 800 ug/ml). Viability of PBMCs was observed and calculated after staining with Trypan blue and Wright stain. A specific Toll-like receptor (TLR) 4 inhibitor, CLI095 was used to determine whether or not G Procumbens exerts its effects through TLR4. An antagonist of lipopolysaccharides (LPS), polymyxin B, was used to evaluate whether endotoxin properties in G Procumbens contributed to its immunomodulatory activity. Cell culture supernatants were analysed by using Luminex® Human Screening Magnetic Assay. G procumbens exhibited not significantly potent immunomodulatory activity of IL-10 by PBMCs. The expression of IL-10 were increased upon exposure to different concentrations of G procumbens by co-treatment with CLI-095 (TLR4 inhibitor) but partially decreased by polymyxin B (LPS-neutralizer). One-way ANOVA of those treatment groups compared to control group give/?>0.05 followed by post-hoc multiple comparison Dunnett's test. We demonstrate impotent immunomodulatory activity of G procumbens towards the expression of IL-10 by PBMCs. We believe that this immunomodulatory activity is due to endotoxin-like properties of G procumbens via TLR4 receptor and MyD88 or Toll/IL-IR domain-containing adaptor inducing IFN-P (TRIF) - signalling pathways.
format Thesis
qualification_level Bachelor degree
author Danial, Zuraini
spellingShingle Danial, Zuraini
Determination of IL-10 expression in peripheral blood mononuclear cells (PBMCs) treated with aqueous extract of gynura procumbens / Zuraini Danial
author_facet Danial, Zuraini
author_sort Danial, Zuraini
title Determination of IL-10 expression in peripheral blood mononuclear cells (PBMCs) treated with aqueous extract of gynura procumbens / Zuraini Danial
title_short Determination of IL-10 expression in peripheral blood mononuclear cells (PBMCs) treated with aqueous extract of gynura procumbens / Zuraini Danial
title_full Determination of IL-10 expression in peripheral blood mononuclear cells (PBMCs) treated with aqueous extract of gynura procumbens / Zuraini Danial
title_fullStr Determination of IL-10 expression in peripheral blood mononuclear cells (PBMCs) treated with aqueous extract of gynura procumbens / Zuraini Danial
title_full_unstemmed Determination of IL-10 expression in peripheral blood mononuclear cells (PBMCs) treated with aqueous extract of gynura procumbens / Zuraini Danial
title_sort determination of il-10 expression in peripheral blood mononuclear cells (pbmcs) treated with aqueous extract of gynura procumbens / zuraini danial
granting_institution Universiti Teknologi MARA (UiTM)
granting_department Faculty of Health Sciences
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/101717/1/101717.pdf
_version_ 1811769185636712448

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