Chemical screening of endophytic fungi extracts by using HPLC (to create a library of endophytic fungi extracts) / Azni Azira Ahmad

This study was aimed to carry out a high performance liquid chromatography analysis of endophytic fungi extracts to determine their secondary metabolites pattern. In a gradient elution at a flow rate of I mL/minute, using a mixture of water-ACN as a mobile phase, reverse phase column as a stationary...

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Main Author:	Ahmad, Azni Azira
Format:	Thesis
Language:	English
Published:	2008
Subjects:	Formularies Collected prescriptions Pharmaceutical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/101874/1/101874.PDF
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id	my-uitm-ir.101874
record_format	uketd_dc
spelling	my-uitm-ir.1018742024-09-11T19:15:40Z Chemical screening of endophytic fungi extracts by using HPLC (to create a library of endophytic fungi extracts) / Azni Azira Ahmad 2008 Ahmad, Azni Azira Formularies. Collected prescriptions Pharmaceutical chemistry This study was aimed to carry out a high performance liquid chromatography analysis of endophytic fungi extracts to determine their secondary metabolites pattern. In a gradient elution at a flow rate of I mL/minute, using a mixture of water-ACN as a mobile phase, reverse phase column as a stationary phase and automated injections of IO µL of samples, secondary metabolites were eluted with retention time from 5 min to 30 min. Diode array detection at 210 nm was used to analyse the HPLC chromatograms of the extracts. The chromatograms obtained demonstrated that some of the extracts present at least one unique unknown secondary metabolite that could be of interest. Fraction collections of extracts were also done in this study. Fractions were collected by time (every 0.5 minutes) between the retention time of 5 min and 30 min to yield 46 fractions. This will be useful in further study regarding these fungi extracts. The extracts were tested against selected bacteria and fungi by using disc diffusion method to determine their antimicrobial activities. All the data collected were included into the database in order to create a library of endophytic fungi extracts to be used in further research. 2008 Thesis https://ir.uitm.edu.my/id/eprint/101874/ https://ir.uitm.edu.my/id/eprint/101874/1/101874.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Sultan, Sadia
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Sultan, Sadia
topic	Formularies Collected prescriptions Pharmaceutical chemistry
spellingShingle	Formularies Collected prescriptions Pharmaceutical chemistry Ahmad, Azni Azira Chemical screening of endophytic fungi extracts by using HPLC (to create a library of endophytic fungi extracts) / Azni Azira Ahmad
description	This study was aimed to carry out a high performance liquid chromatography analysis of endophytic fungi extracts to determine their secondary metabolites pattern. In a gradient elution at a flow rate of I mL/minute, using a mixture of water-ACN as a mobile phase, reverse phase column as a stationary phase and automated injections of IO µL of samples, secondary metabolites were eluted with retention time from 5 min to 30 min. Diode array detection at 210 nm was used to analyse the HPLC chromatograms of the extracts. The chromatograms obtained demonstrated that some of the extracts present at least one unique unknown secondary metabolite that could be of interest. Fraction collections of extracts were also done in this study. Fractions were collected by time (every 0.5 minutes) between the retention time of 5 min and 30 min to yield 46 fractions. This will be useful in further study regarding these fungi extracts. The extracts were tested against selected bacteria and fungi by using disc diffusion method to determine their antimicrobial activities. All the data collected were included into the database in order to create a library of endophytic fungi extracts to be used in further research.
format	Thesis
qualification_level	Bachelor degree
author	Ahmad, Azni Azira
author_facet	Ahmad, Azni Azira
author_sort	Ahmad, Azni Azira
title	Chemical screening of endophytic fungi extracts by using HPLC (to create a library of endophytic fungi extracts) / Azni Azira Ahmad
title_short	Chemical screening of endophytic fungi extracts by using HPLC (to create a library of endophytic fungi extracts) / Azni Azira Ahmad
title_full	Chemical screening of endophytic fungi extracts by using HPLC (to create a library of endophytic fungi extracts) / Azni Azira Ahmad
title_fullStr	Chemical screening of endophytic fungi extracts by using HPLC (to create a library of endophytic fungi extracts) / Azni Azira Ahmad
title_full_unstemmed	Chemical screening of endophytic fungi extracts by using HPLC (to create a library of endophytic fungi extracts) / Azni Azira Ahmad
title_sort	chemical screening of endophytic fungi extracts by using hplc (to create a library of endophytic fungi extracts) / azni azira ahmad
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2008
url	https://ir.uitm.edu.my/id/eprint/101874/1/101874.PDF
_version_	1811769193256714240

Chemical screening of endophytic fungi extracts by using HPLC (to create a library of endophytic fungi extracts) / Azni Azira Ahmad

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