Cloning of the human thiopurine methyltransferase (TMPT) gene fragment for generation of copy numbers in absolute real time PCR / Ahmad Firdaus Mohd Azhar

Thiopurine Methyltransferase (TPMT) is an enzyme involved in the metabolism of thiopurine drugs. The enzyme has been found to be polymorphic resulting in wide phannacokinetic and pharmacodynamic differences among patients. Outcome of therapy has been correlated with the expression of protein in diff...

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Main Author:	Mohd Azhar, Ahmad Firdaus
Format:	Thesis
Language:	English
Published:	2008
Subjects:	Pharmaceutical ethics Pharmaceutical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/101896/1/101896.PDF
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id	my-uitm-ir.101896
record_format	uketd_dc
spelling	my-uitm-ir.1018962024-09-28T23:34:59Z Cloning of the human thiopurine methyltransferase (TMPT) gene fragment for generation of copy numbers in absolute real time PCR / Ahmad Firdaus Mohd Azhar 2008 Mohd Azhar, Ahmad Firdaus Pharmaceutical ethics Pharmaceutical chemistry Thiopurine Methyltransferase (TPMT) is an enzyme involved in the metabolism of thiopurine drugs. The enzyme has been found to be polymorphic resulting in wide phannacokinetic and pharmacodynamic differences among patients. Outcome of therapy has been correlated with the expression of protein in different patients. A quantitative PCR method which measures the expression of gene is thus useful in clinical setting. Patients prescribed thiopurine should be monitored with respect to the protein expression for appropriate dosing of drugs. The aim of the study is to clone the TP MI' gene for use in quantitative PCR process. The clone would be used for the generation of calibration curve to allow an absolute quantitative PCR method to be developed. Specific amplification of the gene of interest was performed by specific primer designed. Subsequently, the DNA encoding for the correct gene was transformed into E-co/i. Specific primer was designed and the gene of interest was successfully amplified. The bacterial are successfully grown in the agar plate but the screening process does not show positive result in the colony selected. 2008 Thesis https://ir.uitm.edu.my/id/eprint/101896/ https://ir.uitm.edu.my/id/eprint/101896/1/101896.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Salleh, Mohd Zaki
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Salleh, Mohd Zaki
topic	Pharmaceutical ethics Pharmaceutical chemistry
spellingShingle	Pharmaceutical ethics Pharmaceutical chemistry Mohd Azhar, Ahmad Firdaus Cloning of the human thiopurine methyltransferase (TMPT) gene fragment for generation of copy numbers in absolute real time PCR / Ahmad Firdaus Mohd Azhar
description	Thiopurine Methyltransferase (TPMT) is an enzyme involved in the metabolism of thiopurine drugs. The enzyme has been found to be polymorphic resulting in wide phannacokinetic and pharmacodynamic differences among patients. Outcome of therapy has been correlated with the expression of protein in different patients. A quantitative PCR method which measures the expression of gene is thus useful in clinical setting. Patients prescribed thiopurine should be monitored with respect to the protein expression for appropriate dosing of drugs. The aim of the study is to clone the TP MI' gene for use in quantitative PCR process. The clone would be used for the generation of calibration curve to allow an absolute quantitative PCR method to be developed. Specific amplification of the gene of interest was performed by specific primer designed. Subsequently, the DNA encoding for the correct gene was transformed into E-co/i. Specific primer was designed and the gene of interest was successfully amplified. The bacterial are successfully grown in the agar plate but the screening process does not show positive result in the colony selected.
format	Thesis
qualification_level	Bachelor degree
author	Mohd Azhar, Ahmad Firdaus
author_facet	Mohd Azhar, Ahmad Firdaus
author_sort	Mohd Azhar, Ahmad Firdaus
title	Cloning of the human thiopurine methyltransferase (TMPT) gene fragment for generation of copy numbers in absolute real time PCR / Ahmad Firdaus Mohd Azhar
title_short	Cloning of the human thiopurine methyltransferase (TMPT) gene fragment for generation of copy numbers in absolute real time PCR / Ahmad Firdaus Mohd Azhar
title_full	Cloning of the human thiopurine methyltransferase (TMPT) gene fragment for generation of copy numbers in absolute real time PCR / Ahmad Firdaus Mohd Azhar
title_fullStr	Cloning of the human thiopurine methyltransferase (TMPT) gene fragment for generation of copy numbers in absolute real time PCR / Ahmad Firdaus Mohd Azhar
title_full_unstemmed	Cloning of the human thiopurine methyltransferase (TMPT) gene fragment for generation of copy numbers in absolute real time PCR / Ahmad Firdaus Mohd Azhar
title_sort	cloning of the human thiopurine methyltransferase (tmpt) gene fragment for generation of copy numbers in absolute real time pcr / ahmad firdaus mohd azhar
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2008
url	https://ir.uitm.edu.my/id/eprint/101896/1/101896.PDF
_version_	1811769197326237696

Cloning of the human thiopurine methyltransferase (TMPT) gene fragment for generation of copy numbers in absolute real time PCR / Ahmad Firdaus Mohd Azhar

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