Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan

The main objective of conducting this study is to identify the metabolites of carbamazepine metabolised by CYP3A4 using various concentrations of carbamazepine and CYP3A4 enzymes at different pH condition and investigate the metabolism through spectroscopic technique. A number of processes involved...

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Main Author:	Maruan, Khalidah
Format:	Thesis
Language:	English
Published:	2008
Subjects:	Metabolism
Online Access:	https://ir.uitm.edu.my/id/eprint/101907/1/101907.PDF
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id	my-uitm-ir.101907
record_format	uketd_dc
spelling	my-uitm-ir.1019072024-09-11T19:17:01Z Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan 2008 Maruan, Khalidah Metabolism The main objective of conducting this study is to identify the metabolites of carbamazepine metabolised by CYP3A4 using various concentrations of carbamazepine and CYP3A4 enzymes at different pH condition and investigate the metabolism through spectroscopic technique. A number of processes involved in order to achieve this objective. First process was enzymatic reaction of carbamazepine and followed by extraction and evaporation in order to isolate pure metabolites. The metabolites were further subjected to Thin Layer Chromatography technique for purification and structural characterization by 1H-NMR. Investigation through 1H-NMR identified the metabolic changes in carbamazepine nucleus and the resulting metabolites formed had been identified as 10, 11-dihydro- l 0-hydroxycarbamazepine and I 0, 11- dihydrocarbamazepine. Therefore, with the identification of metabolites formed, this study has accomplished its objective as stated above. 2008 Thesis https://ir.uitm.edu.my/id/eprint/101907/ https://ir.uitm.edu.my/id/eprint/101907/1/101907.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Ali Shah, Syed Adnan
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ali Shah, Syed Adnan
topic	Metabolism
spellingShingle	Metabolism Maruan, Khalidah Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan
description	The main objective of conducting this study is to identify the metabolites of carbamazepine metabolised by CYP3A4 using various concentrations of carbamazepine and CYP3A4 enzymes at different pH condition and investigate the metabolism through spectroscopic technique. A number of processes involved in order to achieve this objective. First process was enzymatic reaction of carbamazepine and followed by extraction and evaporation in order to isolate pure metabolites. The metabolites were further subjected to Thin Layer Chromatography technique for purification and structural characterization by 1H-NMR. Investigation through 1H-NMR identified the metabolic changes in carbamazepine nucleus and the resulting metabolites formed had been identified as 10, 11-dihydro- l 0-hydroxycarbamazepine and I 0, 11- dihydrocarbamazepine. Therefore, with the identification of metabolites formed, this study has accomplished its objective as stated above.
format	Thesis
qualification_level	Bachelor degree
author	Maruan, Khalidah
author_facet	Maruan, Khalidah
author_sort	Maruan, Khalidah
title	Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan
title_short	Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan
title_full	Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan
title_fullStr	Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan
title_full_unstemmed	Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan
title_sort	carbamazepine metabolism by cyp3a4: in vitro studies using nmr spectroscopy / khalidah maruan
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2008
url	https://ir.uitm.edu.my/id/eprint/101907/1/101907.PDF
_version_	1811769199138177024

Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan

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