Investigation of in vitro metabolism of carbamazepine using CYP2C9 enzymes through 1H-NMR spectroscopic method / Raudhatun Sa' Adiah Ithnin

The main objective of this in vitro study were plan to identify major Carbamazepine metabolites formed in association with CYP2C9 enzymes respectively using variable such as different concentration of Carbamazepine, pH of medium and concentration of CYP2C9 enzymes. Other goals were to identify the s...

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Main Author:	Ithnin, Raudhatun Sa' Adiah
Format:	Thesis
Language:	English
Published:	2008
Subjects:	Metabolism Organic substances
Online Access:	https://ir.uitm.edu.my/id/eprint/102008/1/102008.PDF
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id	my-uitm-ir.102008
record_format	uketd_dc
spelling	my-uitm-ir.1020082024-09-11T19:16:30Z Investigation of in vitro metabolism of carbamazepine using CYP2C9 enzymes through 1H-NMR spectroscopic method / Raudhatun Sa' Adiah Ithnin 2008 Ithnin, Raudhatun Sa' Adiah Metabolism Organic substances The main objective of this in vitro study were plan to identify major Carbamazepine metabolites formed in association with CYP2C9 enzymes respectively using variable such as different concentration of Carbamazepine, pH of medium and concentration of CYP2C9 enzymes. Other goals were to identify the structural changes during metabolism of Carbamazepine compound. There are several techniques involved in order to achieve the goals of this project. The first process involves enzymatic reactions of Carbamazepine. The reaction samples were subjected to extract and evaporate to get pure extract. Obtained extract were further subjected to Thin Layer Chromatographic (TLC) techniques in order to identify transformed metabolites. The suspected metabolites detected on TLC plates were sent for characterization as long as identification using 1H-NMR spectrometer. Two compounds were detected namely, compound (2) and (3) respective to pH 5.8 and 7.4. Concisely, even though the compounds are not main metabolites in metabolism of Carbamazepine as stated in various literatures, we managed to identify new potential compounds that may have possible contribution in various toxicity problem of Carbamazepine. 2008 Thesis https://ir.uitm.edu.my/id/eprint/102008/ https://ir.uitm.edu.my/id/eprint/102008/1/102008.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Ali Shah, Syed Adnan
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ali Shah, Syed Adnan
topic	Metabolism Organic substances
spellingShingle	Metabolism Organic substances Ithnin, Raudhatun Sa' Adiah Investigation of in vitro metabolism of carbamazepine using CYP2C9 enzymes through 1H-NMR spectroscopic method / Raudhatun Sa' Adiah Ithnin
description	The main objective of this in vitro study were plan to identify major Carbamazepine metabolites formed in association with CYP2C9 enzymes respectively using variable such as different concentration of Carbamazepine, pH of medium and concentration of CYP2C9 enzymes. Other goals were to identify the structural changes during metabolism of Carbamazepine compound. There are several techniques involved in order to achieve the goals of this project. The first process involves enzymatic reactions of Carbamazepine. The reaction samples were subjected to extract and evaporate to get pure extract. Obtained extract were further subjected to Thin Layer Chromatographic (TLC) techniques in order to identify transformed metabolites. The suspected metabolites detected on TLC plates were sent for characterization as long as identification using 1H-NMR spectrometer. Two compounds were detected namely, compound (2) and (3) respective to pH 5.8 and 7.4. Concisely, even though the compounds are not main metabolites in metabolism of Carbamazepine as stated in various literatures, we managed to identify new potential compounds that may have possible contribution in various toxicity problem of Carbamazepine.
format	Thesis
qualification_level	Bachelor degree
author	Ithnin, Raudhatun Sa' Adiah
author_facet	Ithnin, Raudhatun Sa' Adiah
author_sort	Ithnin, Raudhatun Sa' Adiah
title	Investigation of in vitro metabolism of carbamazepine using CYP2C9 enzymes through 1H-NMR spectroscopic method / Raudhatun Sa' Adiah Ithnin
title_short	Investigation of in vitro metabolism of carbamazepine using CYP2C9 enzymes through 1H-NMR spectroscopic method / Raudhatun Sa' Adiah Ithnin
title_full	Investigation of in vitro metabolism of carbamazepine using CYP2C9 enzymes through 1H-NMR spectroscopic method / Raudhatun Sa' Adiah Ithnin
title_fullStr	Investigation of in vitro metabolism of carbamazepine using CYP2C9 enzymes through 1H-NMR spectroscopic method / Raudhatun Sa' Adiah Ithnin
title_full_unstemmed	Investigation of in vitro metabolism of carbamazepine using CYP2C9 enzymes through 1H-NMR spectroscopic method / Raudhatun Sa' Adiah Ithnin
title_sort	investigation of in vitro metabolism of carbamazepine using cyp2c9 enzymes through 1h-nmr spectroscopic method / raudhatun sa' adiah ithnin
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2008
url	https://ir.uitm.edu.my/id/eprint/102008/1/102008.PDF
_version_	1811769203207700480

Investigation of in vitro metabolism of carbamazepine using CYP2C9 enzymes through 1H-NMR spectroscopic method / Raudhatun Sa' Adiah Ithnin

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