Micropropagation and callus induction of rice (oryza sativa l.) varieties MR 219 and MR 253 / Nazatul Asikin Muda

Two local Malaysian rice varieties, MR 219 and MR 253 were examined for their in vitro regeneration and callus induction responses using mature seeds, leaf disc, stem, internode and root sections obtained from in vitro seedlings. After 4 weeks of culture, the optimum in vitro regeneration of MR 219...

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Main Author:	Muda, Nazatul Asikin
Format:	Thesis
Language:	English
Published:	2013
Online Access:	https://ir.uitm.edu.my/id/eprint/102049/1/102049.pdf
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id	my-uitm-ir.102049
record_format	uketd_dc
spelling	my-uitm-ir.1020492024-09-12T16:12:30Z Micropropagation and callus induction of rice (oryza sativa l.) varieties MR 219 and MR 253 / Nazatul Asikin Muda 2013 Muda, Nazatul Asikin Two local Malaysian rice varieties, MR 219 and MR 253 were examined for their in vitro regeneration and callus induction responses using mature seeds, leaf disc, stem, internode and root sections obtained from in vitro seedlings. After 4 weeks of culture, the optimum in vitro regeneration of MR 219 (100%) and MR 253 (100%) were recorded from internode explant cultured on MS media supplemented with 2.0 mg/L BAP and 0.05 mg/L NAA and MS media supplemented with 1.0 mg/L BAP and 0.5 mg/L NAA respectively. Other explants failed to regenerate in vitro. In general, rice variety MR 253 showed better in vitro regeneration than MR 219. Callus growth from seed, stem, intemode and root explants became visible after 1-2 weeks being cultured on MS medium supplemented with various concentrations of 2,4-D. The highest callus induction frequencies for MR 219 (100%) and MR 253 (100%) were both obtained from rice seed explant cultured on MS media with 1.5 mg/L 2,4-D. Based on the overall result obtained, rice variety MR 219 gave better callus induction response than MR 253 in terms of callus induction frequency, size and the morphological characteristic of calli induced. Plantlets of MR 219 and MR 253 were successfully acclimatized and transferred onto different planting media. The highest rate of plantlet survival of MR 219 (100%) and MR 253 (60%) during acclimatization was recorded on the mixture of cocoa peat vermiculite and cocoa peat zeolite planting media respectively. 2013 Thesis https://ir.uitm.edu.my/id/eprint/102049/ https://ir.uitm.edu.my/id/eprint/102049/1/102049.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Plantation and Agrotechnology
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
description	Two local Malaysian rice varieties, MR 219 and MR 253 were examined for their in vitro regeneration and callus induction responses using mature seeds, leaf disc, stem, internode and root sections obtained from in vitro seedlings. After 4 weeks of culture, the optimum in vitro regeneration of MR 219 (100%) and MR 253 (100%) were recorded from internode explant cultured on MS media supplemented with 2.0 mg/L BAP and 0.05 mg/L NAA and MS media supplemented with 1.0 mg/L BAP and 0.5 mg/L NAA respectively. Other explants failed to regenerate in vitro. In general, rice variety MR 253 showed better in vitro regeneration than MR 219. Callus growth from seed, stem, intemode and root explants became visible after 1-2 weeks being cultured on MS medium supplemented with various concentrations of 2,4-D. The highest callus induction frequencies for MR 219 (100%) and MR 253 (100%) were both obtained from rice seed explant cultured on MS media with 1.5 mg/L 2,4-D. Based on the overall result obtained, rice variety MR 219 gave better callus induction response than MR 253 in terms of callus induction frequency, size and the morphological characteristic of calli induced. Plantlets of MR 219 and MR 253 were successfully acclimatized and transferred onto different planting media. The highest rate of plantlet survival of MR 219 (100%) and MR 253 (60%) during acclimatization was recorded on the mixture of cocoa peat vermiculite and cocoa peat zeolite planting media respectively.
format	Thesis
qualification_level	Master's degree
author	Muda, Nazatul Asikin
spellingShingle	Muda, Nazatul Asikin Micropropagation and callus induction of rice (oryza sativa l.) varieties MR 219 and MR 253 / Nazatul Asikin Muda
author_facet	Muda, Nazatul Asikin
author_sort	Muda, Nazatul Asikin
title	Micropropagation and callus induction of rice (oryza sativa l.) varieties MR 219 and MR 253 / Nazatul Asikin Muda
title_short	Micropropagation and callus induction of rice (oryza sativa l.) varieties MR 219 and MR 253 / Nazatul Asikin Muda
title_full	Micropropagation and callus induction of rice (oryza sativa l.) varieties MR 219 and MR 253 / Nazatul Asikin Muda
title_fullStr	Micropropagation and callus induction of rice (oryza sativa l.) varieties MR 219 and MR 253 / Nazatul Asikin Muda
title_full_unstemmed	Micropropagation and callus induction of rice (oryza sativa l.) varieties MR 219 and MR 253 / Nazatul Asikin Muda
title_sort	micropropagation and callus induction of rice (oryza sativa l.) varieties mr 219 and mr 253 / nazatul asikin muda
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Plantation and Agrotechnology
publishDate	2013
url	https://ir.uitm.edu.my/id/eprint/102049/1/102049.pdf
_version_	1811769207292952576

Micropropagation and callus induction of rice (oryza sativa l.) varieties MR 219 and MR 253 / Nazatul Asikin Muda

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