NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman

This project is aimed to identify metabolites of carbamazepine by action of CYP206 enzyme. Carbamazepine has numbers of side effects. It is believed that metabolites of carbamazepine are responsible for the occurrence of its side effects although the mechanism underlying the reactions is not known....

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Main Author:	Rahman, Norsuryahani
Format:	Thesis
Language:	English
Published:	2008
Subjects:	Organic chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/103906/1/103906.PDF
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id	my-uitm-ir.103906
record_format	uketd_dc
spelling	my-uitm-ir.1039062024-09-28T23:35:25Z NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman 2008 Rahman, Norsuryahani Organic chemistry This project is aimed to identify metabolites of carbamazepine by action of CYP206 enzyme. Carbamazepine has numbers of side effects. It is believed that metabolites of carbamazepine are responsible for the occurrence of its side effects although the mechanism underlying the reactions is not known. Thus, this research project will investigate carbamazepine metabolism by using biological catalyst. Different concentrations of carbamazepine were used with different concentrations of CYP206 enzyme in two differents pH of media. After incubation, the reaction mixtures were extracted using chloroform. The residues were subjected to analysis using chromatographic and spectroscopic technique. The transformed metabolites of carbamazepine were purified through thin layer chromatography (TLC) and structure metabolites were elucidated through nuclear magnetic resonance (NMR) spectroscopy. As a result of carbamazepine metabolism by CYP206 enzyme, the metabolites being produced are 10, 11-dihydroxycarbamazepine and 10, 11-dihydroxy-10-hydroxycarbamazepine. Even though the compounds are not main metabolites in metabolism of carbamazepine as stated in various literatures, we managed to identify new potential compounds that may have possible contribution in various toxicity problem of carbamazepine. 2008 Thesis https://ir.uitm.edu.my/id/eprint/103906/ https://ir.uitm.edu.my/id/eprint/103906/1/103906.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Ali Shah, Syed Adnan
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ali Shah, Syed Adnan
topic	Organic chemistry
spellingShingle	Organic chemistry Rahman, Norsuryahani NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman
description	This project is aimed to identify metabolites of carbamazepine by action of CYP206 enzyme. Carbamazepine has numbers of side effects. It is believed that metabolites of carbamazepine are responsible for the occurrence of its side effects although the mechanism underlying the reactions is not known. Thus, this research project will investigate carbamazepine metabolism by using biological catalyst. Different concentrations of carbamazepine were used with different concentrations of CYP206 enzyme in two differents pH of media. After incubation, the reaction mixtures were extracted using chloroform. The residues were subjected to analysis using chromatographic and spectroscopic technique. The transformed metabolites of carbamazepine were purified through thin layer chromatography (TLC) and structure metabolites were elucidated through nuclear magnetic resonance (NMR) spectroscopy. As a result of carbamazepine metabolism by CYP206 enzyme, the metabolites being produced are 10, 11-dihydroxycarbamazepine and 10, 11-dihydroxy-10-hydroxycarbamazepine. Even though the compounds are not main metabolites in metabolism of carbamazepine as stated in various literatures, we managed to identify new potential compounds that may have possible contribution in various toxicity problem of carbamazepine.
format	Thesis
qualification_level	Bachelor degree
author	Rahman, Norsuryahani
author_facet	Rahman, Norsuryahani
author_sort	Rahman, Norsuryahani
title	NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman
title_short	NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman
title_full	NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman
title_fullStr	NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman
title_full_unstemmed	NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman
title_sort	nmr-based identification of carbamazepine metabolism through cyp2d6 enzyme / norsuryahani rahman
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2008
url	https://ir.uitm.edu.my/id/eprint/103906/1/103906.PDF
_version_	1811769270458122240

NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman

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