HPLC determination of vitamin C in commercial vitamin C tablets / Nurhusna Abu Bakar
In this study, HPLC technique was used for the quantitative determination of vitamin C in vitamin C tablets. The sample was pulverized and diluted to the diluting factor of 100 and 10. The samples were separated using C18 reversed-phase high performance liquid chromatography (HPLC) with mobile phase...
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2010
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my-uitm-ir.1040062024-09-30T07:50:26Z HPLC determination of vitamin C in commercial vitamin C tablets / Nurhusna Abu Bakar 2010 Abu Bakar, Nurhusna In this study, HPLC technique was used for the quantitative determination of vitamin C in vitamin C tablets. The sample was pulverized and diluted to the diluting factor of 100 and 10. The samples were separated using C18 reversed-phase high performance liquid chromatography (HPLC) with mobile phase of deionised water and methanol (MeOH) (95:5% (v/v)) and mobile phase system with flow rate of 1.0 mL/min and UV detection at wavelength of 265 nm. Vitamin C peak was observed at retention time of approximately 2.126 minutes with total analysis time at about 3 minutes. The calibration curves were prepared for concentration; 0-200 ppm. The calibration curves shown good linearity as r=0.99965. Among five samples of tablets which was brand A, brand B, brand C, brand D and brand E, brand D was fully fulfilled the requirement which less 5% RSD and percent relative error within 10% of label claimed with 4.39% of RSD and 9.75% of relative error. The method was found to be efficient for the analysis of vitamin C in solid sample as it gave good accuracy excellent precision (low value of percent RSD (2.42%). 2010 Thesis https://ir.uitm.edu.my/id/eprint/104006/ https://ir.uitm.edu.my/id/eprint/104006/1/104006.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Applied Science Abdullah@ Mohd Noor, Mashita |
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Universiti Teknologi MARA |
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UiTM Institutional Repository |
| language |
English |
| advisor |
Abdullah@ Mohd Noor, Mashita |
| description |
In this study, HPLC technique was used for the quantitative determination of vitamin C in vitamin C tablets. The sample was pulverized and diluted to the diluting factor of 100 and 10. The samples were separated using C18 reversed-phase high performance liquid chromatography (HPLC) with mobile phase of deionised water and methanol (MeOH) (95:5% (v/v)) and mobile phase system with flow rate of 1.0 mL/min and UV detection at wavelength of 265 nm. Vitamin C peak was observed at retention time of approximately 2.126 minutes with total analysis time at about 3 minutes. The calibration curves were prepared for concentration; 0-200 ppm. The calibration curves shown good linearity as r=0.99965. Among five samples of tablets which was brand A, brand B, brand C, brand D and brand E, brand D was fully fulfilled the requirement which less 5% RSD and percent relative error within 10% of label claimed with 4.39% of RSD and 9.75% of relative error. The method was found to be efficient for the analysis of vitamin C in solid sample as it gave good accuracy excellent precision (low value of percent RSD (2.42%). |
| format |
Thesis |
| qualification_level |
Bachelor degree |
| author |
Abu Bakar, Nurhusna |
| spellingShingle |
Abu Bakar, Nurhusna HPLC determination of vitamin C in commercial vitamin C tablets / Nurhusna Abu Bakar |
| author_facet |
Abu Bakar, Nurhusna |
| author_sort |
Abu Bakar, Nurhusna |
| title |
HPLC determination of vitamin C in commercial vitamin C tablets / Nurhusna Abu Bakar |
| title_short |
HPLC determination of vitamin C in commercial vitamin C tablets / Nurhusna Abu Bakar |
| title_full |
HPLC determination of vitamin C in commercial vitamin C tablets / Nurhusna Abu Bakar |
| title_fullStr |
HPLC determination of vitamin C in commercial vitamin C tablets / Nurhusna Abu Bakar |
| title_full_unstemmed |
HPLC determination of vitamin C in commercial vitamin C tablets / Nurhusna Abu Bakar |
| title_sort |
hplc determination of vitamin c in commercial vitamin c tablets / nurhusna abu bakar |
| granting_institution |
Universiti Teknologi MARA (UiTM) |
| granting_department |
Faculty of Applied Science |
| publishDate |
2010 |
| url |
https://ir.uitm.edu.my/id/eprint/104006/1/104006.pdf |
| _version_ |
1811769277242408960 |