Cloning and transformation of human multidrug resistance gene 1 (MDR1) / Hamidah Mokhtaruddin

Human MDR1 encoded for P-glycoprotein (P-gp) formation. P-gp is an ATP dependent efflux transporter which reduces the intracellular concentration of wide range of drugs including the chemotherapy drugs especially when the MDR1 gene being over expressed. Most of the anticancer agents are the substrat...

Full description

Saved in:

Bibliographic Details
Main Author:	Mokhtaruddin, Hamidah
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Pharmaceutical technology Pharmaceutical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/105121/1/105121.PDF
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.105121
record_format	uketd_dc
spelling	my-uitm-ir.1051212024-10-24T01:46:29Z Cloning and transformation of human multidrug resistance gene 1 (MDR1) / Hamidah Mokhtaruddin 2009 Mokhtaruddin, Hamidah Pharmaceutical technology Pharmaceutical chemistry Human MDR1 encoded for P-glycoprotein (P-gp) formation. P-gp is an ATP dependent efflux transporter which reduces the intracellular concentration of wide range of drugs including the chemotherapy drugs especially when the MDR1 gene being over expressed. Most of the anticancer agents are the substrates of P-gp thus make the tumour cells are able to survive upon exposure to various anticancer and this will lead to chemotherapy resistance. In order to study about the mechanism of P-gp, human MDR1 has been cloned and transformed into plasmid of E.coli as the reaction vector. Before cloning and transformation take place, amplification of MDR1 gene was done using Polymerase Chain Reaction (PCR) and followed by gel electrophoresis. The appeared band on gel with 158 bp showed present of desired gene, thus progressed on cloning and transformation methods. As colonies obtained on the selective agar, positive transformants analysis is followed by doing the second PCR and sequencing. These procedures are to confirm the MDR1 gene is cloned at the correct orientation. 2009 Thesis https://ir.uitm.edu.my/id/eprint/105121/ https://ir.uitm.edu.my/id/eprint/105121/1/105121.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Haslinda, Fazleen
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Haslinda, Fazleen
topic	Pharmaceutical technology Pharmaceutical chemistry
spellingShingle	Pharmaceutical technology Pharmaceutical chemistry Mokhtaruddin, Hamidah Cloning and transformation of human multidrug resistance gene 1 (MDR1) / Hamidah Mokhtaruddin
description	Human MDR1 encoded for P-glycoprotein (P-gp) formation. P-gp is an ATP dependent efflux transporter which reduces the intracellular concentration of wide range of drugs including the chemotherapy drugs especially when the MDR1 gene being over expressed. Most of the anticancer agents are the substrates of P-gp thus make the tumour cells are able to survive upon exposure to various anticancer and this will lead to chemotherapy resistance. In order to study about the mechanism of P-gp, human MDR1 has been cloned and transformed into plasmid of E.coli as the reaction vector. Before cloning and transformation take place, amplification of MDR1 gene was done using Polymerase Chain Reaction (PCR) and followed by gel electrophoresis. The appeared band on gel with 158 bp showed present of desired gene, thus progressed on cloning and transformation methods. As colonies obtained on the selective agar, positive transformants analysis is followed by doing the second PCR and sequencing. These procedures are to confirm the MDR1 gene is cloned at the correct orientation.
format	Thesis
qualification_level	Bachelor degree
author	Mokhtaruddin, Hamidah
author_facet	Mokhtaruddin, Hamidah
author_sort	Mokhtaruddin, Hamidah
title	Cloning and transformation of human multidrug resistance gene 1 (MDR1) / Hamidah Mokhtaruddin
title_short	Cloning and transformation of human multidrug resistance gene 1 (MDR1) / Hamidah Mokhtaruddin
title_full	Cloning and transformation of human multidrug resistance gene 1 (MDR1) / Hamidah Mokhtaruddin
title_fullStr	Cloning and transformation of human multidrug resistance gene 1 (MDR1) / Hamidah Mokhtaruddin
title_full_unstemmed	Cloning and transformation of human multidrug resistance gene 1 (MDR1) / Hamidah Mokhtaruddin
title_sort	cloning and transformation of human multidrug resistance gene 1 (mdr1) / hamidah mokhtaruddin
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2009
url	https://ir.uitm.edu.my/id/eprint/105121/1/105121.PDF
_version_	1818588136810217472

Cloning and transformation of human multidrug resistance gene 1 (MDR1) / Hamidah Mokhtaruddin

Similar Items