Amplification and cloning of NR3 region of NMDA receptor subunit / Nurul Buda Ahmad Shahrir

This thesis focused on the amplification and verification of NR3 subunits of the excitatory glutamate receptor, ionotropic N-methyl-0-aspartate. The functional unit of cation channel gated is believed to be a tetrametric complex structure which consists of NR1, NR2 and NR3 subunits. The glycine bind...

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Main Author:	Ahmad Shahrir, Nurul Buda
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Pharmaceutical industry Pharmaceutical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/105220/1/105220.PDF
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id	my-uitm-ir.105220
record_format	uketd_dc
spelling	my-uitm-ir.1052202024-12-05T09:00:16Z Amplification and cloning of NR3 region of NMDA receptor subunit / Nurul Buda Ahmad Shahrir 2009 Ahmad Shahrir, Nurul Buda Pharmaceutical industry Pharmaceutical chemistry This thesis focused on the amplification and verification of NR3 subunits of the excitatory glutamate receptor, ionotropic N-methyl-0-aspartate. The functional unit of cation channel gated is believed to be a tetrametric complex structure which consists of NR1, NR2 and NR3 subunits. The glycine binding to NR3 subunit is required for the opening of the ion channel. We cloned the mice NR3A and NR3B which has only minor differences in homology to rat NR3 subunit. The RNA was extracted from adult albino mice brain tissue and converted into cDNA by using two steps Reverse-Transcriptase Polymerase Chain Reaction. The NR3A and NR3B were amplified by using specific primer which has been synthesized based on previous studies. The NR3A and NR3B were constructed into pcDNA and pTARGET respectively and cloned into E.coli. Both plasmid then were extracted from E.coli and purified before they were sent for sequencing and verification by using NCBI. Only NR3B was successfully constructed into pT AR GET showing specific region from residue TM1 until S2. Eventhough NR3A was not successfully constructed into pCDNA but the NR3A PCR product was blasted and attributed to specific region C-terminal phosphorylation site. 2009 Thesis https://ir.uitm.edu.my/id/eprint/105220/ https://ir.uitm.edu.my/id/eprint/105220/1/105220.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Abdul Majeed, Abu Bakar
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Abdul Majeed, Abu Bakar
topic	Pharmaceutical industry Pharmaceutical chemistry
spellingShingle	Pharmaceutical industry Pharmaceutical chemistry Ahmad Shahrir, Nurul Buda Amplification and cloning of NR3 region of NMDA receptor subunit / Nurul Buda Ahmad Shahrir
description	This thesis focused on the amplification and verification of NR3 subunits of the excitatory glutamate receptor, ionotropic N-methyl-0-aspartate. The functional unit of cation channel gated is believed to be a tetrametric complex structure which consists of NR1, NR2 and NR3 subunits. The glycine binding to NR3 subunit is required for the opening of the ion channel. We cloned the mice NR3A and NR3B which has only minor differences in homology to rat NR3 subunit. The RNA was extracted from adult albino mice brain tissue and converted into cDNA by using two steps Reverse-Transcriptase Polymerase Chain Reaction. The NR3A and NR3B were amplified by using specific primer which has been synthesized based on previous studies. The NR3A and NR3B were constructed into pcDNA and pTARGET respectively and cloned into E.coli. Both plasmid then were extracted from E.coli and purified before they were sent for sequencing and verification by using NCBI. Only NR3B was successfully constructed into pT AR GET showing specific region from residue TM1 until S2. Eventhough NR3A was not successfully constructed into pCDNA but the NR3A PCR product was blasted and attributed to specific region C-terminal phosphorylation site.
format	Thesis
qualification_level	Bachelor degree
author	Ahmad Shahrir, Nurul Buda
author_facet	Ahmad Shahrir, Nurul Buda
author_sort	Ahmad Shahrir, Nurul Buda
title	Amplification and cloning of NR3 region of NMDA receptor subunit / Nurul Buda Ahmad Shahrir
title_short	Amplification and cloning of NR3 region of NMDA receptor subunit / Nurul Buda Ahmad Shahrir
title_full	Amplification and cloning of NR3 region of NMDA receptor subunit / Nurul Buda Ahmad Shahrir
title_fullStr	Amplification and cloning of NR3 region of NMDA receptor subunit / Nurul Buda Ahmad Shahrir
title_full_unstemmed	Amplification and cloning of NR3 region of NMDA receptor subunit / Nurul Buda Ahmad Shahrir
title_sort	amplification and cloning of nr3 region of nmda receptor subunit / nurul buda ahmad shahrir
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2009
url	https://ir.uitm.edu.my/id/eprint/105220/1/105220.PDF
_version_	1818588141267714048

Amplification and cloning of NR3 region of NMDA receptor subunit / Nurul Buda Ahmad Shahrir

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