Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar
The binary vector is an essential component in Agrobacterium-mediated transformation. It carries the DNA fragments to be delivered into the plant genome. Many binary vectors have been developed for different transformation experiments. Some of the binary vectors are commercially available. Most of t...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2023
|
| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/106824/1/106824.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
my-uitm-ir.106824 |
|---|---|
| record_format |
uketd_dc |
| spelling |
my-uitm-ir.1068242024-12-05T04:41:21Z Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar 2023 Omar, Nawawi Genetics Physical plant physiology The binary vector is an essential component in Agrobacterium-mediated transformation. It carries the DNA fragments to be delivered into the plant genome. Many binary vectors have been developed for different transformation experiments. Some of the binary vectors are commercially available. Most of the binary vectors make use of the viral-derived CaMV 35S promoter and the NOS terminator from bacteria to regulate the expression of the transgene. Besides, the binary vector also contains a selectable marker gene for transformant selection. Using viral-derived regulatory elements has posed particular problems in transgenic plants and raised some biosafety concerns. Therefore, a binary vector containing the plant-derived gene regulatory elements is deemed required. In this study, a new binary vector named pJASIN101 was developed using the backbone of the pMDC162 vector and evaluated in model plants through transient transformation experiments. 2023 Thesis https://ir.uitm.edu.my/id/eprint/106824/ https://ir.uitm.edu.my/id/eprint/106824/1/106824.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Plantation and Agrotechnology |
| institution |
Universiti Teknologi MARA |
| collection |
UiTM Institutional Repository |
| language |
English |
| topic |
Genetics Physical plant physiology |
| spellingShingle |
Genetics Physical plant physiology Omar, Nawawi Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar |
| description |
The binary vector is an essential component in Agrobacterium-mediated transformation. It carries the DNA fragments to be delivered into the plant genome. Many binary vectors have been developed for different transformation experiments. Some of the binary vectors are commercially available. Most of the binary vectors make use of the viral-derived CaMV 35S promoter and the NOS terminator from bacteria to regulate the expression of the transgene. Besides, the binary vector also contains a selectable marker gene for transformant selection. Using viral-derived regulatory elements has posed particular problems in transgenic plants and raised some biosafety concerns. Therefore, a binary vector containing the plant-derived gene regulatory elements is deemed required. In this study, a new binary vector named pJASIN101 was developed using the backbone of the pMDC162 vector and evaluated in model plants through transient transformation experiments. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Omar, Nawawi |
| author_facet |
Omar, Nawawi |
| author_sort |
Omar, Nawawi |
| title |
Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar |
| title_short |
Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar |
| title_full |
Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar |
| title_fullStr |
Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar |
| title_full_unstemmed |
Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar |
| title_sort |
development and evaluation of a new binary vector for gene expression in higher plants / nawawi omar |
| granting_institution |
Universiti Teknologi MARA (UiTM) |
| granting_department |
Faculty of Plantation and Agrotechnology |
| publishDate |
2023 |
| url |
https://ir.uitm.edu.my/id/eprint/106824/1/106824.pdf |
| _version_ |
1818588190008672256 |