Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim

The aim of this study is to develop a genetic test using specific primers to defect the polymorphism of the Human Epidermal Receptor (HER-2) gene. PCR is used to amplify a specific region of DNA in order to produce a large number of nearly identical copies. The method uses a heat stable DNA replicat...

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Main Author:	Ibrahim, Nur Saeidah
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Pharmaceutical industry
Online Access:	https://ir.uitm.edu.my/id/eprint/107051/1/107051.PDF
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id	my-uitm-ir.107051
record_format	uketd_dc
spelling	my-uitm-ir.1070512024-12-10T23:12:15Z Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim 2009 Ibrahim, Nur Saeidah Pharmaceutical industry The aim of this study is to develop a genetic test using specific primers to defect the polymorphism of the Human Epidermal Receptor (HER-2) gene. PCR is used to amplify a specific region of DNA in order to produce a large number of nearly identical copies. The method uses a heat stable DNA replication enzyme called a DNA polymerase, the four deoxynucleotide building blocks of DNA and two small single-stranded DNA segments called primers. Polymerase Chain Reaction using first set of primers was done to amplify the specific sequence of HER-2 gene while the second set of primers was used to amplify the allele specific polymorphism site of HER-2 gene. Both have different fragment size. The PCR products were analyzed using the gel electrophoresis to determine the DNA fragment produced. The bands are observed via gel imaging using the ultra violet (UV) light. The required bands were compared to the standard ladder or marker with known fragment size. Result showed one band indicate homozygous while two bands indicate heterozygous gene. However, for further confirmation, the product that was obtained in this study should be sent for sequencing. 2009 Thesis https://ir.uitm.edu.my/id/eprint/107051/ https://ir.uitm.edu.my/id/eprint/107051/1/107051.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Teh, Lay Kek
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Teh, Lay Kek
topic	Pharmaceutical industry
spellingShingle	Pharmaceutical industry Ibrahim, Nur Saeidah Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim
description	The aim of this study is to develop a genetic test using specific primers to defect the polymorphism of the Human Epidermal Receptor (HER-2) gene. PCR is used to amplify a specific region of DNA in order to produce a large number of nearly identical copies. The method uses a heat stable DNA replication enzyme called a DNA polymerase, the four deoxynucleotide building blocks of DNA and two small single-stranded DNA segments called primers. Polymerase Chain Reaction using first set of primers was done to amplify the specific sequence of HER-2 gene while the second set of primers was used to amplify the allele specific polymorphism site of HER-2 gene. Both have different fragment size. The PCR products were analyzed using the gel electrophoresis to determine the DNA fragment produced. The bands are observed via gel imaging using the ultra violet (UV) light. The required bands were compared to the standard ladder or marker with known fragment size. Result showed one band indicate homozygous while two bands indicate heterozygous gene. However, for further confirmation, the product that was obtained in this study should be sent for sequencing.
format	Thesis
qualification_level	Bachelor degree
author	Ibrahim, Nur Saeidah
author_facet	Ibrahim, Nur Saeidah
author_sort	Ibrahim, Nur Saeidah
title	Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim
title_short	Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim
title_full	Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim
title_fullStr	Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim
title_full_unstemmed	Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim
title_sort	development of a genetic test for detection of polymorphism of human epidermal receptor-2 (her-2) gene / nur saeidah ibrahim
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2009
url	https://ir.uitm.edu.my/id/eprint/107051/1/107051.PDF
_version_	1818588204069027840

Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim

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