Development of a PCR method for the detection of polymorphism of apolipoprotein E gene / Nur Farhana Mohamed Noor

Development of a PCR method for the detection of polymorphism of apolipoprotein E gene / Nur Farhana Mohamed Noor

Apo E gene plays an important role in producing apolipoprotein E that serves as a ligand for uptake by lipoprotein receptor so that cholesterol can be removed from the body. Screening of Apo E gene is important before starting drug therapy as different patient will act differently to the statin ther...

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Main Author: Mohamed Noor, Nur Farhana
Format: Thesis
Language:English
Published: 2009
Subjects:
Pharmaceutical industry
Online Access:https://ir.uitm.edu.my/id/eprint/107168/1/107168.PDF
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spelling my-uitm-ir.1071682024-12-10T23:08:31Z Development of a PCR method for the detection of polymorphism of apolipoprotein E gene / Nur Farhana Mohamed Noor 2009 Mohamed Noor, Nur Farhana Pharmaceutical industry Apo E gene plays an important role in producing apolipoprotein E that serves as a ligand for uptake by lipoprotein receptor so that cholesterol can be removed from the body. Screening of Apo E gene is important before starting drug therapy as different patient will act differently to the statin therapy. The aim of this study is to develop and validate a PCR method for detection of polymorphism of apo E gene. Allele specific PCR together with nested PCR have been used. Two separate amplification are used. First PCR used a set of primers that yield a large product which was used as a template in the second PCR to enhance the sensitivity and specificity of DNA. The PCR was conducted under different annealing temperature to determine the optimal annealing temperature which is 61 °C for the first PCR and 57.6°C for the second PCR. 2009 Thesis https://ir.uitm.edu.my/id/eprint/107168/ https://ir.uitm.edu.my/id/eprint/107168/1/107168.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Teh, Lay Kek
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Teh, Lay Kek
topic Pharmaceutical industry
spellingShingle Pharmaceutical industry
Mohamed Noor, Nur Farhana
Development of a PCR method for the detection of polymorphism of apolipoprotein E gene / Nur Farhana Mohamed Noor
description Apo E gene plays an important role in producing apolipoprotein E that serves as a ligand for uptake by lipoprotein receptor so that cholesterol can be removed from the body. Screening of Apo E gene is important before starting drug therapy as different patient will act differently to the statin therapy. The aim of this study is to develop and validate a PCR method for detection of polymorphism of apo E gene. Allele specific PCR together with nested PCR have been used. Two separate amplification are used. First PCR used a set of primers that yield a large product which was used as a template in the second PCR to enhance the sensitivity and specificity of DNA. The PCR was conducted under different annealing temperature to determine the optimal annealing temperature which is 61 °C for the first PCR and 57.6°C for the second PCR.
format Thesis
qualification_level Bachelor degree
author Mohamed Noor, Nur Farhana
author_facet Mohamed Noor, Nur Farhana
author_sort Mohamed Noor, Nur Farhana
title Development of a PCR method for the detection of polymorphism of apolipoprotein E gene / Nur Farhana Mohamed Noor
title_short Development of a PCR method for the detection of polymorphism of apolipoprotein E gene / Nur Farhana Mohamed Noor
title_full Development of a PCR method for the detection of polymorphism of apolipoprotein E gene / Nur Farhana Mohamed Noor
title_fullStr Development of a PCR method for the detection of polymorphism of apolipoprotein E gene / Nur Farhana Mohamed Noor
title_full_unstemmed Development of a PCR method for the detection of polymorphism of apolipoprotein E gene / Nur Farhana Mohamed Noor
title_sort development of a pcr method for the detection of polymorphism of apolipoprotein e gene / nur farhana mohamed noor
granting_institution Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department Faculty of Pharmacy
publishDate 2009
url https://ir.uitm.edu.my/id/eprint/107168/1/107168.PDF
_version_ 1818588211159498752

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