Curcumin, a natural compound from rhizomes of curcuma longa inhibits cell growth of liver carcinoma cells / Nur Shazwani Mokhtar

Hepatocellular carcinoma is the most common liver cancer form in adults. Curcumin, a non-volatile, yellow colouring agent, contained in turmeric (Curcuma longa L.) was claimed to have anticancer activities on most cancers, including liver cancer.The aim of this study is to evaluate the anticancer ac...

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Main Author:	Mokhtar, Nur Shazwani
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Pharmaceutical industry Pharmaceutical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/107211/1/107211.PDF
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id	my-uitm-ir.107211
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spelling	my-uitm-ir.1072112024-12-10T23:12:36Z Curcumin, a natural compound from rhizomes of curcuma longa inhibits cell growth of liver carcinoma cells / Nur Shazwani Mokhtar 2009 Mokhtar, Nur Shazwani Pharmaceutical industry Pharmaceutical chemistry Hepatocellular carcinoma is the most common liver cancer form in adults. Curcumin, a non-volatile, yellow colouring agent, contained in turmeric (Curcuma longa L.) was claimed to have anticancer activities on most cancers, including liver cancer.The aim of this study is to evaluate the anticancer activities of curcumin from rhizomes of Curcuma longa L. on human liver cancer cells. In this study, two types of cell lines, HepG2 (human hepatocarcinoma) cells and WRL 68 (non-tumourigenic fetal liver) cells were used. Cells were plated in 96-wellplates and incubated in conditions of 95% 02, 5% C02, at 3 7°C. Five concentrations of curcumin extracts; 0.1, 1, 10, 100, and 1000 µg/ml were used and MTS assay was used to measure cell viability. Concentration response curve for ethyl acetate extract of curcumin and methanol extract of curcumin were constructed to determine the effects of concentration on cell viability and to calculate the median inhibitory concentrations (IC50) for each extract. IC50 for curcumin (MeOH) in HepG2 cells and curcumin (EA) in HepG2 cells were 125.3 ± 41.lµg/ml and 146.5± 52.0µg/ml respectively. While, IC50 for curcumin (MeOH) in WRL 68 cells and curcumin (EA) in WRL 68 cells were 224.3 ± 57.2 µg/ml and 263 ± 55.7 µg/ml respectively. The result showed that methanol extract of curcumin was having greater anticancer activity on HepG2 cells. This study can contribute to the development of more effective, safer and cheaper natural based cancer drugs. 2009 Thesis https://ir.uitm.edu.my/id/eprint/107211/ https://ir.uitm.edu.my/id/eprint/107211/1/107211.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Jannah, Noor
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Jannah, Noor
topic	Pharmaceutical industry Pharmaceutical chemistry
spellingShingle	Pharmaceutical industry Pharmaceutical chemistry Mokhtar, Nur Shazwani Curcumin, a natural compound from rhizomes of curcuma longa inhibits cell growth of liver carcinoma cells / Nur Shazwani Mokhtar
description	Hepatocellular carcinoma is the most common liver cancer form in adults. Curcumin, a non-volatile, yellow colouring agent, contained in turmeric (Curcuma longa L.) was claimed to have anticancer activities on most cancers, including liver cancer.The aim of this study is to evaluate the anticancer activities of curcumin from rhizomes of Curcuma longa L. on human liver cancer cells. In this study, two types of cell lines, HepG2 (human hepatocarcinoma) cells and WRL 68 (non-tumourigenic fetal liver) cells were used. Cells were plated in 96-wellplates and incubated in conditions of 95% 02, 5% C02, at 3 7°C. Five concentrations of curcumin extracts; 0.1, 1, 10, 100, and 1000 µg/ml were used and MTS assay was used to measure cell viability. Concentration response curve for ethyl acetate extract of curcumin and methanol extract of curcumin were constructed to determine the effects of concentration on cell viability and to calculate the median inhibitory concentrations (IC50) for each extract. IC50 for curcumin (MeOH) in HepG2 cells and curcumin (EA) in HepG2 cells were 125.3 ± 41.lµg/ml and 146.5± 52.0µg/ml respectively. While, IC50 for curcumin (MeOH) in WRL 68 cells and curcumin (EA) in WRL 68 cells were 224.3 ± 57.2 µg/ml and 263 ± 55.7 µg/ml respectively. The result showed that methanol extract of curcumin was having greater anticancer activity on HepG2 cells. This study can contribute to the development of more effective, safer and cheaper natural based cancer drugs.
format	Thesis
qualification_level	Bachelor degree
author	Mokhtar, Nur Shazwani
author_facet	Mokhtar, Nur Shazwani
author_sort	Mokhtar, Nur Shazwani
title	Curcumin, a natural compound from rhizomes of curcuma longa inhibits cell growth of liver carcinoma cells / Nur Shazwani Mokhtar
title_short	Curcumin, a natural compound from rhizomes of curcuma longa inhibits cell growth of liver carcinoma cells / Nur Shazwani Mokhtar
title_full	Curcumin, a natural compound from rhizomes of curcuma longa inhibits cell growth of liver carcinoma cells / Nur Shazwani Mokhtar
title_fullStr	Curcumin, a natural compound from rhizomes of curcuma longa inhibits cell growth of liver carcinoma cells / Nur Shazwani Mokhtar
title_full_unstemmed	Curcumin, a natural compound from rhizomes of curcuma longa inhibits cell growth of liver carcinoma cells / Nur Shazwani Mokhtar
title_sort	curcumin, a natural compound from rhizomes of curcuma longa inhibits cell growth of liver carcinoma cells / nur shazwani mokhtar
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2009
url	https://ir.uitm.edu.my/id/eprint/107211/1/107211.PDF
_version_	1818588215274110976

Curcumin, a natural compound from rhizomes of curcuma longa inhibits cell growth of liver carcinoma cells / Nur Shazwani Mokhtar

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