Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham

Resistance of breast cancer to radiotherapy is the crucial aspect leading to relapse and low survival rate. Radioresistance is suggested to be linked with epithelial-mesenchymal transition (EMT), a process involved in regulating cancer tissue remodelling, resulting in recurrence and metastasis. The...

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Main Author:	Ronny Sham, Nur Fatihah
Format:	Thesis
Language:	English
Published:	2024
Subjects:	Medical technology Cancer
Online Access:	https://ir.uitm.edu.my/id/eprint/107652/1/107652.pdf
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id	my-uitm-ir.107652
record_format	uketd_dc
spelling	my-uitm-ir.1076522024-12-09T08:31:59Z Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham 2024 Ronny Sham, Nur Fatihah Medical technology Cancer Resistance of breast cancer to radiotherapy is the crucial aspect leading to relapse and low survival rate. Radioresistance is suggested to be linked with epithelial-mesenchymal transition (EMT), a process involved in regulating cancer tissue remodelling, resulting in recurrence and metastasis. The underlying mechanisms in acquiring radioresistance to gamma-ray in mouse mammary cancer cell lines (EMT6) were investigated using both in vitro and in vivo approaches. EMT6 cells were irradiated with gamma-ray at 2 Gy/cycle to initiate the development of radioresistance in vitro. Confirmation of EMT6 cells acquired radioresistance was analysed using migration and clonogenic assays. Next-generation sequencing analysis validated 16 genes of interest (GOI) via real-time polymerase chain reaction (qPCR). The signalling pathways and proteins involved in EMT6 cells acquiring radioresistance were verified by KEGG pathway analysis and western blotting, respectively. EMT6RR_MJI radioresistance cells were developed from parental EMT6 cells after 8 cycles of fractionated gamma-ray irradiation. This is confirmed by irradiation with gamma-ray at 2, 4, and 8 Gy, which resulted in higher survival fractions and migratory rates of EMT6RR_MJI compared to parental cells. 2024 Thesis https://ir.uitm.edu.my/id/eprint/107652/ https://ir.uitm.edu.my/id/eprint/107652/1/107652.pdf text en public phd doctoral Universiti Teknologi MARA (UiTM) Faculty of Medicine
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Medical technology Cancer
spellingShingle	Medical technology Cancer Ronny Sham, Nur Fatihah Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham
description	Resistance of breast cancer to radiotherapy is the crucial aspect leading to relapse and low survival rate. Radioresistance is suggested to be linked with epithelial-mesenchymal transition (EMT), a process involved in regulating cancer tissue remodelling, resulting in recurrence and metastasis. The underlying mechanisms in acquiring radioresistance to gamma-ray in mouse mammary cancer cell lines (EMT6) were investigated using both in vitro and in vivo approaches. EMT6 cells were irradiated with gamma-ray at 2 Gy/cycle to initiate the development of radioresistance in vitro. Confirmation of EMT6 cells acquired radioresistance was analysed using migration and clonogenic assays. Next-generation sequencing analysis validated 16 genes of interest (GOI) via real-time polymerase chain reaction (qPCR). The signalling pathways and proteins involved in EMT6 cells acquiring radioresistance were verified by KEGG pathway analysis and western blotting, respectively. EMT6RR_MJI radioresistance cells were developed from parental EMT6 cells after 8 cycles of fractionated gamma-ray irradiation. This is confirmed by irradiation with gamma-ray at 2, 4, and 8 Gy, which resulted in higher survival fractions and migratory rates of EMT6RR_MJI compared to parental cells.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Ronny Sham, Nur Fatihah
author_facet	Ronny Sham, Nur Fatihah
author_sort	Ronny Sham, Nur Fatihah
title	Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham
title_short	Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham
title_full	Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham
title_fullStr	Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham
title_full_unstemmed	Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham
title_sort	investigation of the pathways involved in the acquired radioresistant emt6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / nur fatihah ronny sham
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Medicine
publishDate	2024
url	https://ir.uitm.edu.my/id/eprint/107652/1/107652.pdf
_version_	1818588229579833344

Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham

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