Chemical constituents of pandanus species / Hannis Fadzillah Mohsin

The Pandanus (family: Pandanaceae) are Asia Pacific plants with approximately 800 species. They are used traditionally for various purposes. Researchers investigate the medicinal values of these plants and interestingly, they provide scientific evidences showing that the plant extracts exhibited bio...

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Main Author:	Mohsin, Hannis Fadzillah
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Organic chemistry Crystallography
Online Access:	https://ir.uitm.edu.my/id/eprint/12973/1/T_HANNIS%20FADZILLAH%20MOHSIN%20PH%2012_24.pdf
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id	my-uitm-ir.12973
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spelling	my-uitm-ir.129732022-07-08T03:25:42Z Chemical constituents of pandanus species / Hannis Fadzillah Mohsin 2012-11 Mohsin, Hannis Fadzillah Organic chemistry Crystallography Botanical chemistry. Phytochemicals The Pandanus (family: Pandanaceae) are Asia Pacific plants with approximately 800 species. They are used traditionally for various purposes. Researchers investigate the medicinal values of these plants and interestingly, they provide scientific evidences showing that the plant extracts exhibited biophannaceutical properties. Thus, this study aims to investigate the plant concerning the phytochemical aspect through its chromatographic profile and analysis using spectroscopy, crystallography and microscopy. This study was conducted on six locally available Pandanus species, namely P. pygmaeus, P. sanderi, P. tectorius, P. dubius, P. conoideus and P. amaryllifolius. Out of these species, only P. amaryllifolius was studied by Malaysian scientists. Meanwhile, three other species (P. tectorius, P. dubius and P. conoideus) were examined by the Philippines and Indonesian counterparts. This work was also emphasized on another two pandans (P. pygmaeus and P. sanderi), which have never been studied before. Thus, the samples were extracted in relatively small scale (less than one kilogram) and the solvents were recycled during the crude extraction. The extractions were based on both acid base and normal procedures, depending upon the targeted compounds. As for the fractionation and isolation, a range of chromatographic techniques were performed such as Silica Gel Column Chromatography (SGCC), preparative Thin Layer Chromatography (TLC), both normal and reverse phase medium, plus high performance liquid chromatographies (HPLC), utilizing ultraviolet detector. Nearly ninety moieties were successfully separated, exclusively based on the HPLC chromatograms. The identification and characterization of pure substituents were accomplished by using the standard Nuclear Magnetic Resonance (NMR) spectroscopic analysis, Time-of-Flight Liquid Chromatography-Mass Spectrometry (TOF LC-MS) and X-ray crystallography of pure crystals. However, merely seven chemical entities were fully recognized from this Pandanus study. Three molecules from P. pygmaeus consist of stigmasterol, lignan and a novel pandan alkaloid, practically called as Pygmaeusamine. Two compounds from P. sanderi include one saponin (lanostane-type) and saponoside (Psitosterol glucoside), respectively. Initial discovery included a compound which was comparable to a tirucallane-type triterpene. However, after a detailed inspection from the X-ray crystallography asessment, it was revealed that the compound has a different carbon skeleton from previously isolated triterpene from Pandanus species and was classified as a lanostane-type triterpene. In the case of P. tectorius, two chemical components were known as a steroid alkaloid and an esterified triterpenoid. Meanwhile, the root of P. amaryllifolius possibly contains the same alkaloid as found in P. tectorius. Still, the isolated moieties from P. dubius and P. conoideus were partially elucidated and cannot be completely characterized due to the insufficient amount of pure samples. In addition, the microscopic analysis revealed the presence of raphide-type crystals of calcium oxalate. In short, this is the first documented occurrence of novel Pandanus alkaloid, steroid alkaloid and saponoside from Pandanus. 2012-11 Thesis https://ir.uitm.edu.my/id/eprint/12973/ https://ir.uitm.edu.my/id/eprint/12973/1/T_HANNIS%20FADZILLAH%20MOHSIN%20PH%2012_24.pdf text en public phd doctoral Universiti Teknologi MARA (UiTM) Faculty of Pharmacy Abdul Wahab, Ibtisam (Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Abdul Wahab, Ibtisam (Dr.)
topic	Organic chemistry Crystallography Organic chemistry
spellingShingle	Organic chemistry Crystallography Organic chemistry Mohsin, Hannis Fadzillah Chemical constituents of pandanus species / Hannis Fadzillah Mohsin
description	The Pandanus (family: Pandanaceae) are Asia Pacific plants with approximately 800 species. They are used traditionally for various purposes. Researchers investigate the medicinal values of these plants and interestingly, they provide scientific evidences showing that the plant extracts exhibited biophannaceutical properties. Thus, this study aims to investigate the plant concerning the phytochemical aspect through its chromatographic profile and analysis using spectroscopy, crystallography and microscopy. This study was conducted on six locally available Pandanus species, namely P. pygmaeus, P. sanderi, P. tectorius, P. dubius, P. conoideus and P. amaryllifolius. Out of these species, only P. amaryllifolius was studied by Malaysian scientists. Meanwhile, three other species (P. tectorius, P. dubius and P. conoideus) were examined by the Philippines and Indonesian counterparts. This work was also emphasized on another two pandans (P. pygmaeus and P. sanderi), which have never been studied before. Thus, the samples were extracted in relatively small scale (less than one kilogram) and the solvents were recycled during the crude extraction. The extractions were based on both acid base and normal procedures, depending upon the targeted compounds. As for the fractionation and isolation, a range of chromatographic techniques were performed such as Silica Gel Column Chromatography (SGCC), preparative Thin Layer Chromatography (TLC), both normal and reverse phase medium, plus high performance liquid chromatographies (HPLC), utilizing ultraviolet detector. Nearly ninety moieties were successfully separated, exclusively based on the HPLC chromatograms. The identification and characterization of pure substituents were accomplished by using the standard Nuclear Magnetic Resonance (NMR) spectroscopic analysis, Time-of-Flight Liquid Chromatography-Mass Spectrometry (TOF LC-MS) and X-ray crystallography of pure crystals. However, merely seven chemical entities were fully recognized from this Pandanus study. Three molecules from P. pygmaeus consist of stigmasterol, lignan and a novel pandan alkaloid, practically called as Pygmaeusamine. Two compounds from P. sanderi include one saponin (lanostane-type) and saponoside (Psitosterol glucoside), respectively. Initial discovery included a compound which was comparable to a tirucallane-type triterpene. However, after a detailed inspection from the X-ray crystallography asessment, it was revealed that the compound has a different carbon skeleton from previously isolated triterpene from Pandanus species and was classified as a lanostane-type triterpene. In the case of P. tectorius, two chemical components were known as a steroid alkaloid and an esterified triterpenoid. Meanwhile, the root of P. amaryllifolius possibly contains the same alkaloid as found in P. tectorius. Still, the isolated moieties from P. dubius and P. conoideus were partially elucidated and cannot be completely characterized due to the insufficient amount of pure samples. In addition, the microscopic analysis revealed the presence of raphide-type crystals of calcium oxalate. In short, this is the first documented occurrence of novel Pandanus alkaloid, steroid alkaloid and saponoside from Pandanus.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Mohsin, Hannis Fadzillah
author_facet	Mohsin, Hannis Fadzillah
author_sort	Mohsin, Hannis Fadzillah
title	Chemical constituents of pandanus species / Hannis Fadzillah Mohsin
title_short	Chemical constituents of pandanus species / Hannis Fadzillah Mohsin
title_full	Chemical constituents of pandanus species / Hannis Fadzillah Mohsin
title_fullStr	Chemical constituents of pandanus species / Hannis Fadzillah Mohsin
title_full_unstemmed	Chemical constituents of pandanus species / Hannis Fadzillah Mohsin
title_sort	chemical constituents of pandanus species / hannis fadzillah mohsin
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Pharmacy
publishDate	2012
url	https://ir.uitm.edu.my/id/eprint/12973/1/T_HANNIS%20FADZILLAH%20MOHSIN%20PH%2012_24.pdf
_version_	1783733170060918784

Chemical constituents of pandanus species / Hannis Fadzillah Mohsin

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