Chemical constituents and biological activities of scaph/ummacropodum (miq.) beumee and sap/umbaccatum (roxb.) / Laode Muhammad Ramadhan Al Muqarrabun

The stem barks of Scaphium macropodum, known as Kembang Semangkuk Jantung (Malay), and Sapium baccatum, known as Ludai (Malay), have been investigated for their phytochemical and pharmacological properties. Several chromatographic techniques were used to separate the chemical compounds including Vac...

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Main Author:	Al Muqarrabun, Laode Muhammad Ramadhan
Format:	Thesis
Language:	English
Published:	2014
Subjects:	Pharmacognosy Pharmaceutical substances (Plant, animal, and inorganic)
Online Access:	https://ir.uitm.edu.my/id/eprint/13998/1/TM_LOADE%20MUHAMMAD%20RAMADHAN%20AL%20MUQARRABUN%20AS%2014_5.pdf
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spelling	my-uitm-ir.139982016-06-10T04:26:37Z Chemical constituents and biological activities of scaph/ummacropodum (miq.) beumee and sap/umbaccatum (roxb.) / Laode Muhammad Ramadhan Al Muqarrabun 2014 Al Muqarrabun, Laode Muhammad Ramadhan Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) The stem barks of Scaphium macropodum, known as Kembang Semangkuk Jantung (Malay), and Sapium baccatum, known as Ludai (Malay), have been investigated for their phytochemical and pharmacological properties. Several chromatographic techniques were used to separate the chemical compounds including Vacuum Liquid Chromatography (VLC), Radial Chromatography (Rf') and Column Chromatography (CC). A new sesquiterpene named malayscaphiol (8M4) along with two lupane triterpenes, lupeol (8Ml) and lupenone (8M2) and stigmasterol (SM3), were successfully isolated from the stem bark of S. macropodum, while a new triterp ene, malaytaraxcrate (8B3), along with two oleanane triterpenes, taraxerol (SBl) and taraxerone (882), docosyl trans-isoferulate (8B4) and docosanoic acid-2',3'dihydroxypropyl ester (8B5) were identified from the stem bark of S. baccatum. The structures of the compounds were determined using several spectroscopic methods, i.e. mass spectrometry (MS), UV-Vis, FT-IR, 10 and 2D NMR including HMQC, HMBC, COSY and NOESY. Several isolated compounds were subjected to cytotoxicity and anti-cholinesterase (anti- AChE) assays to identify their biological properties. Six compounds i.e. 8Ml, 8M2, 8M3, 8B2, 8B4 and SB5 were subjected to cytotoxicity assay. All the tested compounds demonstrated weak anti-cancer activity against HT-29 and MDA-MB cell lines, with SMI and 8M2 exhibited the strongest cytotoxicity among the tested compounds against HT-29 and MDA-MB, respectively. Only 8Ml showed toxic effect towards normal cell line (3T3) with ICso of 38.92 ug/ml., For anti-AChE assay, three compounds were tested i.e. SMl, SM2 and SB2. All the tested compounds exhibited insignificant anti-AChE activity at concentration of 1000 ug/ml., The ICso of the tested compounds could not be determined, because there was only one data point available in this test. Their antiAChE activity were significantly lower than the standard tacrine (ICso== 25 ug/ml.), 2014 Thesis https://ir.uitm.edu.my/id/eprint/13998/ https://ir.uitm.edu.my/id/eprint/13998/1/TM_LOADE%20MUHAMMAD%20RAMADHAN%20AL%20MUQARRABUN%20AS%2014_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Applied Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Pharmacognosy Pharmaceutical substances (Plant, animal, and inorganic)
spellingShingle	Pharmacognosy Pharmaceutical substances (Plant, animal, and inorganic) Al Muqarrabun, Laode Muhammad Ramadhan Chemical constituents and biological activities of scaph/ummacropodum (miq.) beumee and sap/umbaccatum (roxb.) / Laode Muhammad Ramadhan Al Muqarrabun
description	The stem barks of Scaphium macropodum, known as Kembang Semangkuk Jantung (Malay), and Sapium baccatum, known as Ludai (Malay), have been investigated for their phytochemical and pharmacological properties. Several chromatographic techniques were used to separate the chemical compounds including Vacuum Liquid Chromatography (VLC), Radial Chromatography (Rf') and Column Chromatography (CC). A new sesquiterpene named malayscaphiol (8M4) along with two lupane triterpenes, lupeol (8Ml) and lupenone (8M2) and stigmasterol (SM3), were successfully isolated from the stem bark of S. macropodum, while a new triterp ene, malaytaraxcrate (8B3), along with two oleanane triterpenes, taraxerol (SBl) and taraxerone (882), docosyl trans-isoferulate (8B4) and docosanoic acid-2',3'dihydroxypropyl ester (8B5) were identified from the stem bark of S. baccatum. The structures of the compounds were determined using several spectroscopic methods, i.e. mass spectrometry (MS), UV-Vis, FT-IR, 10 and 2D NMR including HMQC, HMBC, COSY and NOESY. Several isolated compounds were subjected to cytotoxicity and anti-cholinesterase (anti- AChE) assays to identify their biological properties. Six compounds i.e. 8Ml, 8M2, 8M3, 8B2, 8B4 and SB5 were subjected to cytotoxicity assay. All the tested compounds demonstrated weak anti-cancer activity against HT-29 and MDA-MB cell lines, with SMI and 8M2 exhibited the strongest cytotoxicity among the tested compounds against HT-29 and MDA-MB, respectively. Only 8Ml showed toxic effect towards normal cell line (3T3) with ICso of 38.92 ug/ml., For anti-AChE assay, three compounds were tested i.e. SMl, SM2 and SB2. All the tested compounds exhibited insignificant anti-AChE activity at concentration of 1000 ug/ml., The ICso of the tested compounds could not be determined, because there was only one data point available in this test. Their antiAChE activity were significantly lower than the standard tacrine (ICso== 25 ug/ml.),
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Al Muqarrabun, Laode Muhammad Ramadhan
author_facet	Al Muqarrabun, Laode Muhammad Ramadhan
author_sort	Al Muqarrabun, Laode Muhammad Ramadhan
title	Chemical constituents and biological activities of scaph/ummacropodum (miq.) beumee and sap/umbaccatum (roxb.) / Laode Muhammad Ramadhan Al Muqarrabun
title_short	Chemical constituents and biological activities of scaph/ummacropodum (miq.) beumee and sap/umbaccatum (roxb.) / Laode Muhammad Ramadhan Al Muqarrabun
title_full	Chemical constituents and biological activities of scaph/ummacropodum (miq.) beumee and sap/umbaccatum (roxb.) / Laode Muhammad Ramadhan Al Muqarrabun
title_fullStr	Chemical constituents and biological activities of scaph/ummacropodum (miq.) beumee and sap/umbaccatum (roxb.) / Laode Muhammad Ramadhan Al Muqarrabun
title_full_unstemmed	Chemical constituents and biological activities of scaph/ummacropodum (miq.) beumee and sap/umbaccatum (roxb.) / Laode Muhammad Ramadhan Al Muqarrabun
title_sort	chemical constituents and biological activities of scaph/ummacropodum (miq.) beumee and sap/umbaccatum (roxb.) / laode muhammad ramadhan al muqarrabun
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2014
url	https://ir.uitm.edu.my/id/eprint/13998/1/TM_LOADE%20MUHAMMAD%20RAMADHAN%20AL%20MUQARRABUN%20AS%2014_5.pdf
_version_	1783733191828307968

Chemical constituents and biological activities of scaph/ummacropodum (miq.) beumee and sap/umbaccatum (roxb.) / Laode Muhammad Ramadhan Al Muqarrabun

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