Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of dicranopteris linearis / Nor Azura Mat Desa

Dicranopteris linearis belongs to the family of Gleicheniaceae known locally in Malay as 'Resam'. Preliminary phytochemical investigation of the crude extracts revealed the presence of flavonoids, saponins, steroids, tannins, and terpenoid. The main aim of this study was to find out the bi...

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Main Author:	Mat Desa, Nor Azura
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Botanical chemistry Phytochemicals RS Pharmacy and materia medica
Online Access:	https://ir.uitm.edu.my/id/eprint/14101/1/TM_NOR%20AZURA%20MAT%20DESA%20PH%2015_5.pdf
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spelling	my-uitm-ir.141012022-03-03T08:19:40Z Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of dicranopteris linearis / Nor Azura Mat Desa 2015 Mat Desa, Nor Azura Botanical chemistry. Phytochemicals RS Pharmacy and materia medica Dicranopteris linearis belongs to the family of Gleicheniaceae known locally in Malay as 'Resam'. Preliminary phytochemical investigation of the crude extracts revealed the presence of flavonoids, saponins, steroids, tannins, and terpenoid. The main aim of this study was to find out the bioactive compounds with potential antimicrobial and cytotoxic activities based on the bioassay-guided fractionation and isolation procedures. Methanol and fractionated extracts (hexane, chloroform and methanol) of D. linearis were investigated for their cytotoxic effects against three cell lines, namely HL60, HCTl16 and MCF7 and one normal cell WRL68 using the MTT assay. The methanol extract was found to be most effective against HL60 (ICso 9.20 ug/ml., respectively). The methanol fraction possessed strong activity against HL60 with ICsoof 12.88 ug/ml. and was fractionated by vacuum liquid chromatography into eleven fractions (FI, F2, F3, F4, F5, F6, F7, F8, F9, FlO and FI1). Only F7 showed the active value with ICso 6.21 ug/ml., Purification of the most bioactive fraction, F7 with column chromatography, radial chromatography and preparative thin layer chromatography resulted in the isolation of four compounds (PCI, PC2, PC3 and PC4). Only PCI and PC2 showed the active value of cytotoxic activity against HL60 with IC50 values of 4.59 and 3.87 ug/ml. respectively. The structure of these compounds were elucidated by spectroscopic methods (lD-NMR, 2-D-NMR, UV, IR and MS) and compared with published data. PCI and PC2 were identified as quercitrin and afzelin, respectively. The micro-broth dilution method was used to determine the antimicrobial activity against S. aureus 25923 (MSSA), S. aureus 33591 (MRSA), B. subtilis 6633, E. coli 35218, P. aeruginosa 27853 and C. albicans 10231. The highest activity was methanol extracts and methanol fraction with the Minimum Inhibitory Concentration (MIC) values 1250 ug/ml, and 625 ug/ml.. All the fractions Fl until F7 showed the weak antimicrobial activity with the MIC >1250 ug/ml., Hence, the separation into single pure compounds was not possible, resulting in a reduced antimicrobial activity. The objectives as outlined were successfully achieved only for cytotoxic activity 2015 Thesis https://ir.uitm.edu.my/id/eprint/14101/ https://ir.uitm.edu.my/id/eprint/14101/1/TM_NOR%20AZURA%20MAT%20DESA%20PH%2015_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Pharmacy
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Botanical chemistry Phytochemicals RS Pharmacy and materia medica
spellingShingle	Botanical chemistry Phytochemicals RS Pharmacy and materia medica Mat Desa, Nor Azura Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of dicranopteris linearis / Nor Azura Mat Desa
description	Dicranopteris linearis belongs to the family of Gleicheniaceae known locally in Malay as 'Resam'. Preliminary phytochemical investigation of the crude extracts revealed the presence of flavonoids, saponins, steroids, tannins, and terpenoid. The main aim of this study was to find out the bioactive compounds with potential antimicrobial and cytotoxic activities based on the bioassay-guided fractionation and isolation procedures. Methanol and fractionated extracts (hexane, chloroform and methanol) of D. linearis were investigated for their cytotoxic effects against three cell lines, namely HL60, HCTl16 and MCF7 and one normal cell WRL68 using the MTT assay. The methanol extract was found to be most effective against HL60 (ICso 9.20 ug/ml., respectively). The methanol fraction possessed strong activity against HL60 with ICsoof 12.88 ug/ml. and was fractionated by vacuum liquid chromatography into eleven fractions (FI, F2, F3, F4, F5, F6, F7, F8, F9, FlO and FI1). Only F7 showed the active value with ICso 6.21 ug/ml., Purification of the most bioactive fraction, F7 with column chromatography, radial chromatography and preparative thin layer chromatography resulted in the isolation of four compounds (PCI, PC2, PC3 and PC4). Only PCI and PC2 showed the active value of cytotoxic activity against HL60 with IC50 values of 4.59 and 3.87 ug/ml. respectively. The structure of these compounds were elucidated by spectroscopic methods (lD-NMR, 2-D-NMR, UV, IR and MS) and compared with published data. PCI and PC2 were identified as quercitrin and afzelin, respectively. The micro-broth dilution method was used to determine the antimicrobial activity against S. aureus 25923 (MSSA), S. aureus 33591 (MRSA), B. subtilis 6633, E. coli 35218, P. aeruginosa 27853 and C. albicans 10231. The highest activity was methanol extracts and methanol fraction with the Minimum Inhibitory Concentration (MIC) values 1250 ug/ml, and 625 ug/ml.. All the fractions Fl until F7 showed the weak antimicrobial activity with the MIC >1250 ug/ml., Hence, the separation into single pure compounds was not possible, resulting in a reduced antimicrobial activity. The objectives as outlined were successfully achieved only for cytotoxic activity
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Mat Desa, Nor Azura
author_facet	Mat Desa, Nor Azura
author_sort	Mat Desa, Nor Azura
title	Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of dicranopteris linearis / Nor Azura Mat Desa
title_short	Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of dicranopteris linearis / Nor Azura Mat Desa
title_full	Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of dicranopteris linearis / Nor Azura Mat Desa
title_fullStr	Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of dicranopteris linearis / Nor Azura Mat Desa
title_full_unstemmed	Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of dicranopteris linearis / Nor Azura Mat Desa
title_sort	bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of dicranopteris linearis / nor azura mat desa
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Pharmacy
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/14101/1/TM_NOR%20AZURA%20MAT%20DESA%20PH%2015_5.pdf
_version_	1783733213488742400

Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of dicranopteris linearis / Nor Azura Mat Desa

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