Polycyclic polyprenylated acylphloroglucinols from the bark of mesua ferrea and their anti-diabetic activity / Nurulfazlina Edayah Rasol

The ethyl acetate extract of the bark of Mesua ferrea from Clusiaceae family was studied for its chemical constituent’s together with a-amylase and a-glucosidase inhibitory activities for potential anti-diabetic properties. The phytochemistry investigation resulted in the isolation of seven new poly...

Full description

Saved in:

Bibliographic Details
Main Author:	Rasol, Nurulfazlina Edayah
Format:	Thesis
Language:	English
Published:	2014
Subjects:	Diabetes Mellitus
Online Access:	https://ir.uitm.edu.my/id/eprint/14325/1/TM_NURULFAZLINA%20EDAYAH%20RASOL%20AS%2014_5.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.14325
record_format	uketd_dc
spelling	my-uitm-ir.143252022-04-01T03:05:37Z Polycyclic polyprenylated acylphloroglucinols from the bark of mesua ferrea and their anti-diabetic activity / Nurulfazlina Edayah Rasol 2014-05 Rasol, Nurulfazlina Edayah Diabetes Mellitus Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) The ethyl acetate extract of the bark of Mesua ferrea from Clusiaceae family was studied for its chemical constituent’s together with a-amylase and a-glucosidase inhibitory activities for potential anti-diabetic properties. The phytochemistry investigation resulted in the isolation of seven new polycyclic polyprenylated acylphloroglucinols of type B(I). Various chromatographic techniques were used in the isolation process. For fractionation, VLC, TLC and MPLC were employed.Purification of the compounds was accomplished using repeated HPLC technique due to very close retention time. Structures of the isolated compounds were established by means of spectroscopic methods which includes ID-NMR (H and ^C), 2D-NMR (COSY, HSQC, HMBC, NOESY and TOCSY), UV, IR and LCMS. The compounds were named mesuaferroic acid A, mesuaferroic acid B, mesuaferroic acid C, mesuaferroic acid D, mesuaferroic acid E, mesuaferroic acid F and mesuaferroic acid G. Polycyclic polyprenylated acylphloroglucinols have never been reported in Mesua species before. All the extracts and isolated compounds were subjected to a-amylase and a-glucosidase inhibitory assays for potential anti-diabetic properties. The results obtained from both assays indicate that the ethyl acetate extract has potential for antidiabetic with moderate a-amylase and strong a-glucosidase inhibitory activity. The hexane extract showed strong inhibition properties for both assays and methanol showed strong a-amylase but did not show a-glucosidase inhibitory activity. Of the seven pure compounds, mesuaferroic acid D, mesuaferroic acid E and mesuaferroic acid F may be regarded as having moderate anti-diabetic property with ICso values of 345.87 ng/ml, 260.97 (xg/ml and 349.50 ^g/ml, respectively in the a-glucosidase inhibition assay as compared to 1-deoxynojirimycin standard IC50 values of 102.82 Hg/ml. 2014-05 Thesis https://ir.uitm.edu.my/id/eprint/14325/ https://ir.uitm.edu.my/id/eprint/14325/1/TM_NURULFAZLINA%20EDAYAH%20RASOL%20AS%2014_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Applied Sciences Ismail, Nor Hadiani (Prof. Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ismail, Nor Hadiani (Prof. Dr.)
topic	Diabetes Mellitus Diabetes Mellitus
spellingShingle	Diabetes Mellitus Diabetes Mellitus Rasol, Nurulfazlina Edayah Polycyclic polyprenylated acylphloroglucinols from the bark of mesua ferrea and their anti-diabetic activity / Nurulfazlina Edayah Rasol
description	The ethyl acetate extract of the bark of Mesua ferrea from Clusiaceae family was studied for its chemical constituent’s together with a-amylase and a-glucosidase inhibitory activities for potential anti-diabetic properties. The phytochemistry investigation resulted in the isolation of seven new polycyclic polyprenylated acylphloroglucinols of type B(I). Various chromatographic techniques were used in the isolation process. For fractionation, VLC, TLC and MPLC were employed.Purification of the compounds was accomplished using repeated HPLC technique due to very close retention time. Structures of the isolated compounds were established by means of spectroscopic methods which includes ID-NMR (H and ^C), 2D-NMR (COSY, HSQC, HMBC, NOESY and TOCSY), UV, IR and LCMS. The compounds were named mesuaferroic acid A, mesuaferroic acid B, mesuaferroic acid C, mesuaferroic acid D, mesuaferroic acid E, mesuaferroic acid F and mesuaferroic acid G. Polycyclic polyprenylated acylphloroglucinols have never been reported in Mesua species before. All the extracts and isolated compounds were subjected to a-amylase and a-glucosidase inhibitory assays for potential anti-diabetic properties. The results obtained from both assays indicate that the ethyl acetate extract has potential for antidiabetic with moderate a-amylase and strong a-glucosidase inhibitory activity. The hexane extract showed strong inhibition properties for both assays and methanol showed strong a-amylase but did not show a-glucosidase inhibitory activity. Of the seven pure compounds, mesuaferroic acid D, mesuaferroic acid E and mesuaferroic acid F may be regarded as having moderate anti-diabetic property with ICso values of 345.87 ng/ml, 260.97 (xg/ml and 349.50 ^g/ml, respectively in the a-glucosidase inhibition assay as compared to 1-deoxynojirimycin standard IC50 values of 102.82 Hg/ml.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Rasol, Nurulfazlina Edayah
author_facet	Rasol, Nurulfazlina Edayah
author_sort	Rasol, Nurulfazlina Edayah
title	Polycyclic polyprenylated acylphloroglucinols from the bark of mesua ferrea and their anti-diabetic activity / Nurulfazlina Edayah Rasol
title_short	Polycyclic polyprenylated acylphloroglucinols from the bark of mesua ferrea and their anti-diabetic activity / Nurulfazlina Edayah Rasol
title_full	Polycyclic polyprenylated acylphloroglucinols from the bark of mesua ferrea and their anti-diabetic activity / Nurulfazlina Edayah Rasol
title_fullStr	Polycyclic polyprenylated acylphloroglucinols from the bark of mesua ferrea and their anti-diabetic activity / Nurulfazlina Edayah Rasol
title_full_unstemmed	Polycyclic polyprenylated acylphloroglucinols from the bark of mesua ferrea and their anti-diabetic activity / Nurulfazlina Edayah Rasol
title_sort	polycyclic polyprenylated acylphloroglucinols from the bark of mesua ferrea and their anti-diabetic activity / nurulfazlina edayah rasol
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2014
url	https://ir.uitm.edu.my/id/eprint/14325/1/TM_NURULFAZLINA%20EDAYAH%20RASOL%20AS%2014_5.pdf
_version_	1783733254662127616

Polycyclic polyprenylated acylphloroglucinols from the bark of mesua ferrea and their anti-diabetic activity / Nurulfazlina Edayah Rasol

Similar Items