Ciproxifan, a Histamine h3 receptor antagonist as a potential therapeutic agent in Alzheimer's disease / Siti Murnirah Jaafar

A Histamine H3 receptor antagonist, ciproxifan has been shown to enhance the release of neurotransmitters which play an important role in cognitive process. It is widely documented that extracellular deposition of ~-amyloidpeptide (A~) plays a causal role in the pathogenesis -of AD. However, the rol...

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Main Author:	Jaafar, Siti Murnirah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Neurophysiology and neuropsychology
Online Access:	https://ir.uitm.edu.my/id/eprint/15149/1/TM_SITI%20MURNIRAH%20JAAFAR%20PH%2015_5.pdf
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id	my-uitm-ir.15149
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spelling	my-uitm-ir.151492022-03-04T01:26:43Z Ciproxifan, a Histamine h3 receptor antagonist as a potential therapeutic agent in Alzheimer's disease / Siti Murnirah Jaafar 2015 Jaafar, Siti Murnirah Neurophysiology and neuropsychology A Histamine H3 receptor antagonist, ciproxifan has been shown to enhance the release of neurotransmitters which play an important role in cognitive process. It is widely documented that extracellular deposition of ~-amyloidpeptide (A~) plays a causal role in the pathogenesis -of AD. However, the role of ciproxifan on A~ has not been well documented. The present study was aimed to investigate the ability of ciproxifan to inhibit BACE-l activity and to determine the neuroprotective effect of ciproxifan on A~ in vitro. Furthermore, the effect of ciproxifan on the AD related biomarkers was also investigated using APP(Sw) transgenic mice of AD model. The BACE-l inhibition activity was carried out using BACE-l assay kit while the SK-N-SH cells were induced with AP25-35 prior to the treatment with ciproxifan and then cell viability as well as ROS assay also was determined. For in vivo study, ciproxifan was administrated to the mice by intraperitoneal injection (i.p) for 15 days with two selective doses (1 and 3 mg/kg) and spatial learning and memory behaviour were assessed using radial arm maze (RAM). Brain tissues were collected to measure acetylcholine (ACh), acetylcholinesterase (AChE), nitric oxide (NO), lipid peroxidation (LPO), antioxidant activities, cyclooxygenase (COX) and proinflammatory cytokines assay while plasma were collected to measure an antiinflammatory cytokine TGF-I p. The results for in vitro study demonstrated that ciproxifan weakly inhibited BACE-I activity with IC50BAcE 500 11g/m1 and showed neuroprotective effect by increasing the cell viability and inhibited the production of ROS and these effects were comparable with positive control u-tocopherol.Meanwhile, ciproxifan significantly reduced time taken of the mouse to consume all five baits, working memory error and reference memory error in RAM. Ciproxifan did not show any alteration on the level of both ABI-40 and A~142 in APP transgenic mice.Ciproxifan also elevated the level of ACh while reduced AChE activity and showed anti-oxidant properties by reducing NO and LPG levels as well as enhancing the level of antioxidants (catalase, aSH and GPx). Moreover, the results of neuroinflammatory analysis showed that ciproxifan reduced both COX-I and COX-2 activities, decreased the level of pro-inflammatory cytokines IL-I (1, IL-I ~ and IL-6 and increased the level of anti-inflammatory cytokine TGF-I~. In conclusion, the present study suggests that ciproxifan possessed neuroprotective effect against A~ and could protect the SK-NSH cells from Ajl-induced toxicity by preventing the cell death through the inhibition of oxidative stress. However, protective effects of ciproxifan probably were not through the inhibition of BACE-lactivity and the reduction of Ap level but by other mechanism. The ameliorative effect of ciproxifan on memory deficit of APP transgenic mice may be mediated through improving cholinergic, antioxidant and antiinflammatory activities. This present study may provide some scientific evidences of ciproxifan through various mechanisms as a promising agent for the treatment of AD 2015 Thesis https://ir.uitm.edu.my/id/eprint/15149/ https://ir.uitm.edu.my/id/eprint/15149/1/TM_SITI%20MURNIRAH%20JAAFAR%20PH%2015_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Pharmacy
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Neurophysiology and neuropsychology
spellingShingle	Neurophysiology and neuropsychology Jaafar, Siti Murnirah Ciproxifan, a Histamine h3 receptor antagonist as a potential therapeutic agent in Alzheimer's disease / Siti Murnirah Jaafar
description	A Histamine H3 receptor antagonist, ciproxifan has been shown to enhance the release of neurotransmitters which play an important role in cognitive process. It is widely documented that extracellular deposition of ~-amyloidpeptide (A~) plays a causal role in the pathogenesis -of AD. However, the role of ciproxifan on A~ has not been well documented. The present study was aimed to investigate the ability of ciproxifan to inhibit BACE-l activity and to determine the neuroprotective effect of ciproxifan on A~ in vitro. Furthermore, the effect of ciproxifan on the AD related biomarkers was also investigated using APP(Sw) transgenic mice of AD model. The BACE-l inhibition activity was carried out using BACE-l assay kit while the SK-N-SH cells were induced with AP25-35 prior to the treatment with ciproxifan and then cell viability as well as ROS assay also was determined. For in vivo study, ciproxifan was administrated to the mice by intraperitoneal injection (i.p) for 15 days with two selective doses (1 and 3 mg/kg) and spatial learning and memory behaviour were assessed using radial arm maze (RAM). Brain tissues were collected to measure acetylcholine (ACh), acetylcholinesterase (AChE), nitric oxide (NO), lipid peroxidation (LPO), antioxidant activities, cyclooxygenase (COX) and proinflammatory cytokines assay while plasma were collected to measure an antiinflammatory cytokine TGF-I p. The results for in vitro study demonstrated that ciproxifan weakly inhibited BACE-I activity with IC50BAcE 500 11g/m1 and showed neuroprotective effect by increasing the cell viability and inhibited the production of ROS and these effects were comparable with positive control u-tocopherol.Meanwhile, ciproxifan significantly reduced time taken of the mouse to consume all five baits, working memory error and reference memory error in RAM. Ciproxifan did not show any alteration on the level of both ABI-40 and A~142 in APP transgenic mice.Ciproxifan also elevated the level of ACh while reduced AChE activity and showed anti-oxidant properties by reducing NO and LPG levels as well as enhancing the level of antioxidants (catalase, aSH and GPx). Moreover, the results of neuroinflammatory analysis showed that ciproxifan reduced both COX-I and COX-2 activities, decreased the level of pro-inflammatory cytokines IL-I (1, IL-I ~ and IL-6 and increased the level of anti-inflammatory cytokine TGF-I~. In conclusion, the present study suggests that ciproxifan possessed neuroprotective effect against A~ and could protect the SK-NSH cells from Ajl-induced toxicity by preventing the cell death through the inhibition of oxidative stress. However, protective effects of ciproxifan probably were not through the inhibition of BACE-lactivity and the reduction of Ap level but by other mechanism. The ameliorative effect of ciproxifan on memory deficit of APP transgenic mice may be mediated through improving cholinergic, antioxidant and antiinflammatory activities. This present study may provide some scientific evidences of ciproxifan through various mechanisms as a promising agent for the treatment of AD
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Jaafar, Siti Murnirah
author_facet	Jaafar, Siti Murnirah
author_sort	Jaafar, Siti Murnirah
title	Ciproxifan, a Histamine h3 receptor antagonist as a potential therapeutic agent in Alzheimer's disease / Siti Murnirah Jaafar
title_short	Ciproxifan, a Histamine h3 receptor antagonist as a potential therapeutic agent in Alzheimer's disease / Siti Murnirah Jaafar
title_full	Ciproxifan, a Histamine h3 receptor antagonist as a potential therapeutic agent in Alzheimer's disease / Siti Murnirah Jaafar
title_fullStr	Ciproxifan, a Histamine h3 receptor antagonist as a potential therapeutic agent in Alzheimer's disease / Siti Murnirah Jaafar
title_full_unstemmed	Ciproxifan, a Histamine h3 receptor antagonist as a potential therapeutic agent in Alzheimer's disease / Siti Murnirah Jaafar
title_sort	ciproxifan, a histamine h3 receptor antagonist as a potential therapeutic agent in alzheimer's disease / siti murnirah jaafar
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Pharmacy
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/15149/1/TM_SITI%20MURNIRAH%20JAAFAR%20PH%2015_5.pdf
_version_	1783733349330714624

Ciproxifan, a Histamine h3 receptor antagonist as a potential therapeutic agent in Alzheimer's disease / Siti Murnirah Jaafar

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