Isolation, identification and characterization of microorganisms involved in rubber odour production / Nornanysya Feroze Khan

The presence of obnoxious odour is due to microbial reactions that could lead to harmful effects to the environment. This study was aimed to investigate the correlation between microorganisms and rubber odour production. There were 23 isolates possibly producing the odour isolated from various sampl...

Full description

Saved in:

Bibliographic Details
Main Author:	Feroze Khan, Nornanysya
Format:	Thesis
Language:	English
Published:	2013
Subjects:	Microbial ecology
Online Access:	https://ir.uitm.edu.my/id/eprint/15163/1/TM_NORNANYSYA%20FEROZE%20KHAN%20%20AS%2013_5.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.15163
record_format	uketd_dc
spelling	my-uitm-ir.151632022-04-20T05:26:03Z Isolation, identification and characterization of microorganisms involved in rubber odour production / Nornanysya Feroze Khan 2013-10 Feroze Khan, Nornanysya Microbial ecology The presence of obnoxious odour is due to microbial reactions that could lead to harmful effects to the environment. This study was aimed to investigate the correlation between microorganisms and rubber odour production. There were 23 isolates possibly producing the odour isolated from various sampling points of rubber plantation at Felda Kg. Awah, Temerloh Pahang and were identified using VITEK®2 System analysis. From the observation, Kocuria kristinae was found to be the common species in this study since it was isolated at all sampling sites. Furthermore, 15 isolates were selected for analysis of rubber odour production using solid-phase microextraction (SPME) and Gas Chromatography Mass Spectrometry (GC-MS). The analysis showed that, the volatile components detected comprised of hydrocarbon and low molecular weight compounds such as volatile fatty acids, amino and sulphurcontaining compound, esters and alcohol. There were 18 odorous compounds detected in this study mainly comprised of 5H-Naphto[2,3-c] carbazole,5-methyl-; 7HDibenzo[b,g] carbazole,7-methyl and camphene. However, in order to suppress the growth of microorganism that lead to the production of odorous compounds, three different antimicrobial agents (Sodium hypochlorite, Chloroxylenol and Formaldehyde) as well as HISKA® were used. The treated rubber was subjected to SPME/GCMS analysis to check for the absence, of volatile components. The analysis showed that 0.5% (w/v) and 1.0% (w/v) formaldehyde followed by 1.0% (w/v) and 3.0% (w/v) sodium hypochlorite were able to inhibit the microbial growth and at the same time eliminate the unpleasant odour. The cure characteristics and physical properties of the vulcanized rubber base on ACS 1 mix formulation were evaluated. The treated rubber showed a better quality, hence enhanced the physical properties such as tensile strength, hardness and resilience when compared to SMR L. 2013-10 Thesis https://ir.uitm.edu.my/id/eprint/15163/ https://ir.uitm.edu.my/id/eprint/15163/1/TM_NORNANYSYA%20FEROZE%20KHAN%20%20AS%2013_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Applied Sciences Syed Mohamad, Sharifah Aminah (Prof. Madya Dr.) Samsuri, Azemi (Prof. Dr.) Kamarun, Dzaraini (Assoc. Prof. Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Syed Mohamad, Sharifah Aminah (Prof. Madya Dr.) Samsuri, Azemi (Prof. Dr.) Kamarun, Dzaraini (Assoc. Prof. Dr.)
topic	Microbial ecology
spellingShingle	Microbial ecology Feroze Khan, Nornanysya Isolation, identification and characterization of microorganisms involved in rubber odour production / Nornanysya Feroze Khan
description	The presence of obnoxious odour is due to microbial reactions that could lead to harmful effects to the environment. This study was aimed to investigate the correlation between microorganisms and rubber odour production. There were 23 isolates possibly producing the odour isolated from various sampling points of rubber plantation at Felda Kg. Awah, Temerloh Pahang and were identified using VITEK®2 System analysis. From the observation, Kocuria kristinae was found to be the common species in this study since it was isolated at all sampling sites. Furthermore, 15 isolates were selected for analysis of rubber odour production using solid-phase microextraction (SPME) and Gas Chromatography Mass Spectrometry (GC-MS). The analysis showed that, the volatile components detected comprised of hydrocarbon and low molecular weight compounds such as volatile fatty acids, amino and sulphurcontaining compound, esters and alcohol. There were 18 odorous compounds detected in this study mainly comprised of 5H-Naphto[2,3-c] carbazole,5-methyl-; 7HDibenzo[b,g] carbazole,7-methyl and camphene. However, in order to suppress the growth of microorganism that lead to the production of odorous compounds, three different antimicrobial agents (Sodium hypochlorite, Chloroxylenol and Formaldehyde) as well as HISKA® were used. The treated rubber was subjected to SPME/GCMS analysis to check for the absence, of volatile components. The analysis showed that 0.5% (w/v) and 1.0% (w/v) formaldehyde followed by 1.0% (w/v) and 3.0% (w/v) sodium hypochlorite were able to inhibit the microbial growth and at the same time eliminate the unpleasant odour. The cure characteristics and physical properties of the vulcanized rubber base on ACS 1 mix formulation were evaluated. The treated rubber showed a better quality, hence enhanced the physical properties such as tensile strength, hardness and resilience when compared to SMR L.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Feroze Khan, Nornanysya
author_facet	Feroze Khan, Nornanysya
author_sort	Feroze Khan, Nornanysya
title	Isolation, identification and characterization of microorganisms involved in rubber odour production / Nornanysya Feroze Khan
title_short	Isolation, identification and characterization of microorganisms involved in rubber odour production / Nornanysya Feroze Khan
title_full	Isolation, identification and characterization of microorganisms involved in rubber odour production / Nornanysya Feroze Khan
title_fullStr	Isolation, identification and characterization of microorganisms involved in rubber odour production / Nornanysya Feroze Khan
title_full_unstemmed	Isolation, identification and characterization of microorganisms involved in rubber odour production / Nornanysya Feroze Khan
title_sort	isolation, identification and characterization of microorganisms involved in rubber odour production / nornanysya feroze khan
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2013
url	https://ir.uitm.edu.my/id/eprint/15163/1/TM_NORNANYSYA%20FEROZE%20KHAN%20%20AS%2013_5.pdf
_version_	1783733352322301952

Isolation, identification and characterization of microorganisms involved in rubber odour production / Nornanysya Feroze Khan

Similar Items