Biological activities of four varieties of ficus deltoidea (jack) and correlation to their flavonoid content / Syazwani Dzolin

In this study, the aqueous leaves and figs extracts of four varieties of Ficus deltoidea (Fd) namely Fdvk (kunstleri), Fdva (angustifolia), Fdvd (deltoidea) and Fdvi {intermedia) were estimated for their total phenolic and total flavonoid contents, and evaluated for their in vitro antioxidant, a-glu...

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Main Author: Dzolin, Syazwani
Format: Thesis
Language:English
Published: 2014
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Online Access:https://ir.uitm.edu.my/id/eprint/15280/1/TM_SYAZWANI%20DZOLIN%20AS%2014_5.pdf
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spelling my-uitm-ir.152802022-03-14T04:12:58Z Biological activities of four varieties of ficus deltoidea (jack) and correlation to their flavonoid content / Syazwani Dzolin 2014 Dzolin, Syazwani Plant anatomy In this study, the aqueous leaves and figs extracts of four varieties of Ficus deltoidea (Fd) namely Fdvk (kunstleri), Fdva (angustifolia), Fdvd (deltoidea) and Fdvi {intermedia) were estimated for their total phenolic and total flavonoid contents, and evaluated for their in vitro antioxidant, a-glucosidase inhibitory and neuroprotective effects. Their flavonoid constituents and their correlation with their biological activities were also investigated. The TPC and TFC assays estimated that high content of total phenolics (89.10-99.60 mg GAE/g extract) for extracts of Fdvd and Fdvi but total flavonoids content was significantly higher in Fdvd. In the FTC antioxidant assay, the leaves and figs extracts of both Fdvd and Fdvi showed high percent of inhibition of more than 95% in the FTC assay and 73-79% in the TBA assay. The figs extracts of these two varieties (Fdvd and Fdvi) also exhibited strong radical scavenging activity in the DPPH assay, with ICsoof 7.8 |ig/ml. Interestingly, only the leaves extract of both varieties displayed higher inhibition towards a-glucosidase (Fdvd > Fdvi > Fdvk > Fdva) compared to the other two varieties and compared to the figs extract indicating the presence of more antidiabetic constituents in the leaves. In the neurotoxicity assay, all samples exhibited no signs of toxicity (> 50% cell viability) against SH-SY5Y neuroblastoma cell and displayed potential neuroprotective effects against H202-indueed toxicity cells. Fdvd and Fdvi (leaves and figs) showed good neuroprotective potential indicated by high percent of cell viability as determined by the MTS assay. Following chromatography of extracts on a reversed phase Cis column, LCMS Q-TOF identified the marker flavonoids of the plants from their m/z values as well as by comparison with internal standards. Employing the HCA technique, a dendrogram representing four clusters of flavonoids distribution in the eight samples (based on its distribution values) comprising ep/catechin, quercetin-3- rutinoside, quercetin 5,4'-di-0-beta-Z)-glucopyranoside, myricetin and naringenin were successfully generated. A good correlation was observed between the flavonoid distributions in each plant variety to its biological activities. In conclusion, this study found that the strong activity of Fdvd and Fdvi could be correlated to the presence of the antioxidant epz'catechin relatively high amounts in both varieties. 2014 Thesis https://ir.uitm.edu.my/id/eprint/15280/ https://ir.uitm.edu.my/id/eprint/15280/1/TM_SYAZWANI%20DZOLIN%20AS%2014_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty Applied Sciences
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
topic Plant anatomy
spellingShingle Plant anatomy
Dzolin, Syazwani
Biological activities of four varieties of ficus deltoidea (jack) and correlation to their flavonoid content / Syazwani Dzolin
description In this study, the aqueous leaves and figs extracts of four varieties of Ficus deltoidea (Fd) namely Fdvk (kunstleri), Fdva (angustifolia), Fdvd (deltoidea) and Fdvi {intermedia) were estimated for their total phenolic and total flavonoid contents, and evaluated for their in vitro antioxidant, a-glucosidase inhibitory and neuroprotective effects. Their flavonoid constituents and their correlation with their biological activities were also investigated. The TPC and TFC assays estimated that high content of total phenolics (89.10-99.60 mg GAE/g extract) for extracts of Fdvd and Fdvi but total flavonoids content was significantly higher in Fdvd. In the FTC antioxidant assay, the leaves and figs extracts of both Fdvd and Fdvi showed high percent of inhibition of more than 95% in the FTC assay and 73-79% in the TBA assay. The figs extracts of these two varieties (Fdvd and Fdvi) also exhibited strong radical scavenging activity in the DPPH assay, with ICsoof 7.8 |ig/ml. Interestingly, only the leaves extract of both varieties displayed higher inhibition towards a-glucosidase (Fdvd > Fdvi > Fdvk > Fdva) compared to the other two varieties and compared to the figs extract indicating the presence of more antidiabetic constituents in the leaves. In the neurotoxicity assay, all samples exhibited no signs of toxicity (> 50% cell viability) against SH-SY5Y neuroblastoma cell and displayed potential neuroprotective effects against H202-indueed toxicity cells. Fdvd and Fdvi (leaves and figs) showed good neuroprotective potential indicated by high percent of cell viability as determined by the MTS assay. Following chromatography of extracts on a reversed phase Cis column, LCMS Q-TOF identified the marker flavonoids of the plants from their m/z values as well as by comparison with internal standards. Employing the HCA technique, a dendrogram representing four clusters of flavonoids distribution in the eight samples (based on its distribution values) comprising ep/catechin, quercetin-3- rutinoside, quercetin 5,4'-di-0-beta-Z)-glucopyranoside, myricetin and naringenin were successfully generated. A good correlation was observed between the flavonoid distributions in each plant variety to its biological activities. In conclusion, this study found that the strong activity of Fdvd and Fdvi could be correlated to the presence of the antioxidant epz'catechin relatively high amounts in both varieties.
format Thesis
qualification_name Master of Philosophy (M.Phil.)
qualification_level Master's degree
author Dzolin, Syazwani
author_facet Dzolin, Syazwani
author_sort Dzolin, Syazwani
title Biological activities of four varieties of ficus deltoidea (jack) and correlation to their flavonoid content / Syazwani Dzolin
title_short Biological activities of four varieties of ficus deltoidea (jack) and correlation to their flavonoid content / Syazwani Dzolin
title_full Biological activities of four varieties of ficus deltoidea (jack) and correlation to their flavonoid content / Syazwani Dzolin
title_fullStr Biological activities of four varieties of ficus deltoidea (jack) and correlation to their flavonoid content / Syazwani Dzolin
title_full_unstemmed Biological activities of four varieties of ficus deltoidea (jack) and correlation to their flavonoid content / Syazwani Dzolin
title_sort biological activities of four varieties of ficus deltoidea (jack) and correlation to their flavonoid content / syazwani dzolin
granting_institution Universiti Teknologi MARA
granting_department Faculty Applied Sciences
publishDate 2014
url https://ir.uitm.edu.my/id/eprint/15280/1/TM_SYAZWANI%20DZOLIN%20AS%2014_5.pdf
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