Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah

Rapid increase in the industrial application of nanomaterials such as MgO nanoparticles has great impact on the public. MgO nanomaterial is now used in medical and pharmaceutical products as well as industrial products such as paints.Thus, they are now widely used by the public, but their significan...

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Main Author:	Hasbullah, Nur Intan
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Physical and theoretical chemistry Analytical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/15416/1/TM_NUR%20INTAN%20HASBULLAH%20AS%2015_5.pdf
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spelling	my-uitm-ir.154162016-11-23T06:33:56Z Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah 2015 Hasbullah, Nur Intan Physical and theoretical chemistry Analytical chemistry Rapid increase in the industrial application of nanomaterials such as MgO nanoparticles has great impact on the public. MgO nanomaterial is now used in medical and pharmaceutical products as well as industrial products such as paints.Thus, they are now widely used by the public, but their significant potential risks of exposure have not been systematically investigated. In this study, toxic effects of MgO nanoparticles which differ in terms of size and physical dimensions were investigated against cancer neuroblastoma SH-SY5Y cell lines and neuron-phenotypic cell lines. Viability of cells was measured by using MTS assay. Three types of MgO were used in the study, of which two of them were ultra-thin MgO sheets designated as MgO-5 (size: 500 \|im, 5 nm thickness) and MgO-10 (size: 500 \|im, 2 nm thickness). The other nanoparticle was spherical with average size of 10-100 nm (MgO-24). Oxidative stress was measured by the production of reactive oxygen species (ROS) using dicholofluorescein (DCF) assay. Beneficial effects of MgO nanoparticles were also evaluated for potential neuroprotection. Results indicated that the toxic effects of MgO nanoparticles on cancer neuroblastoma SH-SY5Y cell lines and neuron-phenotypic cell lines were dependent on shape, concentration and size of MgO nanoparticles. Particles with spherical shape (MgO-24) exerted more toxic effects compared to ultra-thin nanosheet particles (MgO-5 and MgO-10). MgO nanoparticles were not toxic to both cell lines at concentration <100 \|ng/ml and did not exert any potential for neuroprotection. However, once the concentration was increased to 1 mg/ml, MgO nanoparticles significantly reduced the viability of both cell lines tested due the production of ROS. 2015 Thesis https://ir.uitm.edu.my/id/eprint/15416/ https://ir.uitm.edu.my/id/eprint/15416/1/TM_NUR%20INTAN%20HASBULLAH%20AS%2015_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Applied Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Physical and theoretical chemistry Analytical chemistry
spellingShingle	Physical and theoretical chemistry Analytical chemistry Hasbullah, Nur Intan Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah
description	Rapid increase in the industrial application of nanomaterials such as MgO nanoparticles has great impact on the public. MgO nanomaterial is now used in medical and pharmaceutical products as well as industrial products such as paints.Thus, they are now widely used by the public, but their significant potential risks of exposure have not been systematically investigated. In this study, toxic effects of MgO nanoparticles which differ in terms of size and physical dimensions were investigated against cancer neuroblastoma SH-SY5Y cell lines and neuron-phenotypic cell lines. Viability of cells was measured by using MTS assay. Three types of MgO were used in the study, of which two of them were ultra-thin MgO sheets designated as MgO-5 (size: 500 \|im, 5 nm thickness) and MgO-10 (size: 500 \|im, 2 nm thickness). The other nanoparticle was spherical with average size of 10-100 nm (MgO-24). Oxidative stress was measured by the production of reactive oxygen species (ROS) using dicholofluorescein (DCF) assay. Beneficial effects of MgO nanoparticles were also evaluated for potential neuroprotection. Results indicated that the toxic effects of MgO nanoparticles on cancer neuroblastoma SH-SY5Y cell lines and neuron-phenotypic cell lines were dependent on shape, concentration and size of MgO nanoparticles. Particles with spherical shape (MgO-24) exerted more toxic effects compared to ultra-thin nanosheet particles (MgO-5 and MgO-10). MgO nanoparticles were not toxic to both cell lines at concentration <100 \|ng/ml and did not exert any potential for neuroprotection. However, once the concentration was increased to 1 mg/ml, MgO nanoparticles significantly reduced the viability of both cell lines tested due the production of ROS.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Hasbullah, Nur Intan
author_facet	Hasbullah, Nur Intan
author_sort	Hasbullah, Nur Intan
title	Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah
title_short	Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah
title_full	Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah
title_fullStr	Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah
title_full_unstemmed	Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah
title_sort	toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / nur intan hasbullah
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/15416/1/TM_NUR%20INTAN%20HASBULLAH%20AS%2015_5.pdf
_version_	1783733391338766336

Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah

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