In vitro regeneration, acclimatizationand fruit quality assessments of rockmelon (Cucumis melo) cv. Glamour / Saiyidah Nafisah Hashim

Rockmelon cv. Glamour (Cucumis melo) is a fruit plant which belongs to Cucurbitaceae family that has great economic value, high resistance to diseases and able to produce large fruit. In this research, regeneration protocol through in vitro culture technique was developed by using different concentr...

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Main Author:	Hashim, Saiyidah Nafisah
Format:	Thesis
Language:	English
Published:	2014
Subjects:	Medical botany (General)
Online Access:	https://ir.uitm.edu.my/id/eprint/15557/1/TM_SAIYIDAH%20NAFISAH%20HASHIM%20AS%2014_5.PDF
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id	my-uitm-ir.15557
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spelling	my-uitm-ir.155572022-03-30T04:53:29Z In vitro regeneration, acclimatizationand fruit quality assessments of rockmelon (Cucumis melo) cv. Glamour / Saiyidah Nafisah Hashim 2014-03 Hashim, Saiyidah Nafisah Medical botany (General) Rockmelon cv. Glamour (Cucumis melo) is a fruit plant which belongs to Cucurbitaceae family that has great economic value, high resistance to diseases and able to produce large fruit. In this research, regeneration protocol through in vitro culture technique was developed by using different concentrations of hormones and coconut water (CW). Then, the plantlets were further acclimatized under ex vitro condition. Findings showed that nodal explants had successfully shoot regenerated on all media. In all MS media combined with CW and cytokinin, there were synergistic effects on shoot regeneration of explant. The best media for multiple shoots regenerating was obtained from MS medium combined with 0.1 mg/L kinetin and 15% CW (K0.1/15). The medium also gave the best plant growth performance with highest shoots length (2.07±1.47 cm), number of leaves (10.30±6.65) and number of nodal (2.26±1.43). In root induction media, MSO media gave the highest number of roots (10.3±2.41), root length (13.5±2.07 cm) and plantlet length (6.30±1.57 cm). For acclimatization, plantlet grown in vermiculite growth media showed the highest survival rate (81±23.09 %). Plants were further grown and fruits were harvested after 2 to 3 months. Fruit quality assessments resulted no significant differences (P>0.05) in the fruit fresh weight, fruit diameter, P-carotene and carbohydrate contents for both grown plants. In vitro grown plant did not show abnormalities which might indicate variation or mutation occurrence during the micropropagation procedures. In conclusion, direct shoot regeneration of rockmelon cv. Glamour were successfully constructed and plant had generated same quality level of fruits with in vivo grown plant. 2014-03 Thesis https://ir.uitm.edu.my/id/eprint/15557/ https://ir.uitm.edu.my/id/eprint/15557/1/TM_SAIYIDAH%20NAFISAH%20HASHIM%20AS%2014_5.PDF text en public mphil masters Universiti Teknologi MARA Faculty of Applied Sciences Jaafar Sidik, Norrizah (Dr.) Abdullah, Shamsiah (Dr. )
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Jaafar Sidik, Norrizah (Dr.) Abdullah, Shamsiah (Dr. )
topic	Medical botany (General)
spellingShingle	Medical botany (General) Hashim, Saiyidah Nafisah In vitro regeneration, acclimatizationand fruit quality assessments of rockmelon (Cucumis melo) cv. Glamour / Saiyidah Nafisah Hashim
description	Rockmelon cv. Glamour (Cucumis melo) is a fruit plant which belongs to Cucurbitaceae family that has great economic value, high resistance to diseases and able to produce large fruit. In this research, regeneration protocol through in vitro culture technique was developed by using different concentrations of hormones and coconut water (CW). Then, the plantlets were further acclimatized under ex vitro condition. Findings showed that nodal explants had successfully shoot regenerated on all media. In all MS media combined with CW and cytokinin, there were synergistic effects on shoot regeneration of explant. The best media for multiple shoots regenerating was obtained from MS medium combined with 0.1 mg/L kinetin and 15% CW (K0.1/15). The medium also gave the best plant growth performance with highest shoots length (2.07±1.47 cm), number of leaves (10.30±6.65) and number of nodal (2.26±1.43). In root induction media, MSO media gave the highest number of roots (10.3±2.41), root length (13.5±2.07 cm) and plantlet length (6.30±1.57 cm). For acclimatization, plantlet grown in vermiculite growth media showed the highest survival rate (81±23.09 %). Plants were further grown and fruits were harvested after 2 to 3 months. Fruit quality assessments resulted no significant differences (P>0.05) in the fruit fresh weight, fruit diameter, P-carotene and carbohydrate contents for both grown plants. In vitro grown plant did not show abnormalities which might indicate variation or mutation occurrence during the micropropagation procedures. In conclusion, direct shoot regeneration of rockmelon cv. Glamour were successfully constructed and plant had generated same quality level of fruits with in vivo grown plant.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Hashim, Saiyidah Nafisah
author_facet	Hashim, Saiyidah Nafisah
author_sort	Hashim, Saiyidah Nafisah
title	In vitro regeneration, acclimatizationand fruit quality assessments of rockmelon (Cucumis melo) cv. Glamour / Saiyidah Nafisah Hashim
title_short	In vitro regeneration, acclimatizationand fruit quality assessments of rockmelon (Cucumis melo) cv. Glamour / Saiyidah Nafisah Hashim
title_full	In vitro regeneration, acclimatizationand fruit quality assessments of rockmelon (Cucumis melo) cv. Glamour / Saiyidah Nafisah Hashim
title_fullStr	In vitro regeneration, acclimatizationand fruit quality assessments of rockmelon (Cucumis melo) cv. Glamour / Saiyidah Nafisah Hashim
title_full_unstemmed	In vitro regeneration, acclimatizationand fruit quality assessments of rockmelon (Cucumis melo) cv. Glamour / Saiyidah Nafisah Hashim
title_sort	in vitro regeneration, acclimatizationand fruit quality assessments of rockmelon (cucumis melo) cv. glamour / saiyidah nafisah hashim
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2014
url	https://ir.uitm.edu.my/id/eprint/15557/1/TM_SAIYIDAH%20NAFISAH%20HASHIM%20AS%2014_5.PDF
_version_	1783733421983399936

In vitro regeneration, acclimatizationand fruit quality assessments of rockmelon (Cucumis melo) cv. Glamour / Saiyidah Nafisah Hashim

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