Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal
This thesis describes a novel method for the structural characterization of oligostilbenes based on high performance liquid chromatography combined with electrospray ionization ion trap multiple-stage mass spectrometry. Pure oligostilbenes as well as a standard heartwood extract from Neobalanocarpus...
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my-uitm-ir.156322022-03-04T01:11:41Z Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal 2015 Jalal, Ratni Suriyani Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) This thesis describes a novel method for the structural characterization of oligostilbenes based on high performance liquid chromatography combined with electrospray ionization ion trap multiple-stage mass spectrometry. Pure oligostilbenes as well as a standard heartwood extract from Neobalanocarpus heimii were analyzed using MS1 to MS5 positive ion mode. The MS" data of [M+H]+ ion of oligostilbenes were found to provide fragmentation patterns unique of each oligostilbene. The essential part of the reported method is the referencing of mass spectral data that allows for comparison of fragmentation patterns between different oligostilbenes based on multiple stage mass spectra. Fragmentation mechanisms were proposed for the most common fragmentation patterns. A pilot database including ESI-IT-MS" data for 11 oligostilbenes structures was established and used to dereplicate bark and leave extracts from Neobalanocarpus heimii. Components of these extracts were efficiently identified within a very short analysis time, thus demonstrating the power of this HPLC on-line coupled MS" approach. The method was evaluated with respect to available hardware and software and directions for further development were suggested. 2015 Thesis https://ir.uitm.edu.my/id/eprint/15632/ https://ir.uitm.edu.my/id/eprint/15632/1/TM_RATNI%20SURIYANI%20JALAL%20PH%2015_5.PDF text en public mphil masters Universiti Teknologi MARA Faculty of Pharmacy |
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Universiti Teknologi MARA |
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UiTM Institutional Repository |
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Pharmacognosy Pharmaceutical substances (Plant, animal, and inorganic) |
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Pharmacognosy Pharmaceutical substances (Plant, animal, and inorganic) Jalal, Ratni Suriyani Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal |
description |
This thesis describes a novel method for the structural characterization of oligostilbenes based on high performance liquid chromatography combined with electrospray ionization ion trap multiple-stage mass spectrometry. Pure oligostilbenes as well as a standard heartwood extract from Neobalanocarpus heimii were analyzed using MS1 to MS5 positive ion mode. The MS" data of [M+H]+ ion of oligostilbenes were found to provide fragmentation patterns unique of each oligostilbene. The essential part of the reported method is the referencing of mass spectral data that allows for comparison of fragmentation patterns between different oligostilbenes based on multiple stage mass spectra. Fragmentation mechanisms were proposed for the most common fragmentation patterns. A pilot database including ESI-IT-MS" data for 11 oligostilbenes structures was established and used to dereplicate bark and leave extracts from Neobalanocarpus heimii. Components of these extracts were efficiently identified within a very short analysis time, thus demonstrating the power of this
HPLC on-line coupled MS" approach. The method was evaluated with respect to available hardware and software and directions for further development were suggested. |
format |
Thesis |
qualification_name |
Master of Philosophy (M.Phil.) |
qualification_level |
Master's degree |
author |
Jalal, Ratni Suriyani |
author_facet |
Jalal, Ratni Suriyani |
author_sort |
Jalal, Ratni Suriyani |
title |
Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal |
title_short |
Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal |
title_full |
Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal |
title_fullStr |
Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal |
title_full_unstemmed |
Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal |
title_sort |
characterization of oligostilbenes from bark and leaves of neobalanocarpus heimii using ion trap lc-ms/ms" technique / ratni suriyani jalal |
granting_institution |
Universiti Teknologi MARA |
granting_department |
Faculty of Pharmacy |
publishDate |
2015 |
url |
https://ir.uitm.edu.my/id/eprint/15632/1/TM_RATNI%20SURIYANI%20JALAL%20PH%2015_5.PDF |
_version_ |
1783733433565970432 |