Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal

This thesis describes a novel method for the structural characterization of oligostilbenes based on high performance liquid chromatography combined with electrospray ionization ion trap multiple-stage mass spectrometry. Pure oligostilbenes as well as a standard heartwood extract from Neobalanocarpus...

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Main Author:	Jalal, Ratni Suriyani
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Pharmacognosy Pharmaceutical substances (Plant, animal, and inorganic)
Online Access:	https://ir.uitm.edu.my/id/eprint/15632/1/TM_RATNI%20SURIYANI%20JALAL%20PH%2015_5.PDF
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id	my-uitm-ir.15632
record_format	uketd_dc
spelling	my-uitm-ir.156322022-03-04T01:11:41Z Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal 2015 Jalal, Ratni Suriyani Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) This thesis describes a novel method for the structural characterization of oligostilbenes based on high performance liquid chromatography combined with electrospray ionization ion trap multiple-stage mass spectrometry. Pure oligostilbenes as well as a standard heartwood extract from Neobalanocarpus heimii were analyzed using MS1 to MS5 positive ion mode. The MS" data of [M+H]+ ion of oligostilbenes were found to provide fragmentation patterns unique of each oligostilbene. The essential part of the reported method is the referencing of mass spectral data that allows for comparison of fragmentation patterns between different oligostilbenes based on multiple stage mass spectra. Fragmentation mechanisms were proposed for the most common fragmentation patterns. A pilot database including ESI-IT-MS" data for 11 oligostilbenes structures was established and used to dereplicate bark and leave extracts from Neobalanocarpus heimii. Components of these extracts were efficiently identified within a very short analysis time, thus demonstrating the power of this HPLC on-line coupled MS" approach. The method was evaluated with respect to available hardware and software and directions for further development were suggested. 2015 Thesis https://ir.uitm.edu.my/id/eprint/15632/ https://ir.uitm.edu.my/id/eprint/15632/1/TM_RATNI%20SURIYANI%20JALAL%20PH%2015_5.PDF text en public mphil masters Universiti Teknologi MARA Faculty of Pharmacy
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Pharmacognosy Pharmaceutical substances (Plant, animal, and inorganic)
spellingShingle	Pharmacognosy Pharmaceutical substances (Plant, animal, and inorganic) Jalal, Ratni Suriyani Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal
description	This thesis describes a novel method for the structural characterization of oligostilbenes based on high performance liquid chromatography combined with electrospray ionization ion trap multiple-stage mass spectrometry. Pure oligostilbenes as well as a standard heartwood extract from Neobalanocarpus heimii were analyzed using MS1 to MS5 positive ion mode. The MS" data of [M+H]+ ion of oligostilbenes were found to provide fragmentation patterns unique of each oligostilbene. The essential part of the reported method is the referencing of mass spectral data that allows for comparison of fragmentation patterns between different oligostilbenes based on multiple stage mass spectra. Fragmentation mechanisms were proposed for the most common fragmentation patterns. A pilot database including ESI-IT-MS" data for 11 oligostilbenes structures was established and used to dereplicate bark and leave extracts from Neobalanocarpus heimii. Components of these extracts were efficiently identified within a very short analysis time, thus demonstrating the power of this HPLC on-line coupled MS" approach. The method was evaluated with respect to available hardware and software and directions for further development were suggested.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Jalal, Ratni Suriyani
author_facet	Jalal, Ratni Suriyani
author_sort	Jalal, Ratni Suriyani
title	Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal
title_short	Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal
title_full	Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal
title_fullStr	Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal
title_full_unstemmed	Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal
title_sort	characterization of oligostilbenes from bark and leaves of neobalanocarpus heimii using ion trap lc-ms/ms" technique / ratni suriyani jalal
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Pharmacy
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/15632/1/TM_RATNI%20SURIYANI%20JALAL%20PH%2015_5.PDF
_version_	1783733433565970432

Characterization of oligostilbenes from bark and leaves of Neobalanocarpus heimii using ion trap LC-MS/MS" technique / Ratni Suriyani Jalal

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