Gene expression of biopsied murine preimplantation embryos / Norhazlin Jusoh @ Mohd Yusoff

Biopsy of embryo is a prerequisite technique employed in Preimplantation Genetic Diagnosis (PGD). Removal of one or two intact blastomeres can be carried out without detriment to embryonic development. However, the effect of piezo-assisted biopsy on gene expression of embryos remains to be elucidate...

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Main Author:	Jusoh @ Mohd Yusoff, Norhazlin
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Radiographic magnification The embryo and fetus
Online Access:	https://ir.uitm.edu.my/id/eprint/15717/1/TM_NORHAZLIN%20JUSOH%20%40%20MOHD%20YUSOFF%20MD%2015_5.pdf
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id	my-uitm-ir.15717
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spelling	my-uitm-ir.157172022-03-10T00:19:52Z Gene expression of biopsied murine preimplantation embryos / Norhazlin Jusoh @ Mohd Yusoff 2015 Jusoh @ Mohd Yusoff, Norhazlin Radiographic magnification The embryo and fetus Biopsy of embryo is a prerequisite technique employed in Preimplantation Genetic Diagnosis (PGD). Removal of one or two intact blastomeres can be carried out without detriment to embryonic development. However, the effect of piezo-assisted biopsy on gene expression of embryos remains to be elucidated. This study was conducted to elucidate the effect of piezo-assisted biopsy on the 8-cell, morula and blastocyst stages on embryonic development, global gene expression and their functional pathways. Following zona breaching by piezo-drilling, single blastomeres were removed from in vz'vo-derived 8-cell ICR mouse embryos. Biopsied embryos were allowed to develop further in Ml6 medium, and collected for RNA extraction after 1 h (8-cell), 16 h (morula) and 42 h (blastocyst). cDNA were converted, amplified, labelled and hybridized on the GeneChip®. Microarray data were analysed using GeneSpring GX 12. Gene expression of non-biopsied and post-biopsied embryos at the 8-cell, morula and blastocyst stages were compared using the t-test with p<0.05, including fold change of >1.5 for each gene. Results showed a significant difference in blastocyst development after piezo-assisted biopsy. A total of 82 genes, 75 genes and 66 genes were significantly different at the 8-cell, morula and blastocyst stages respectively. The differentially expressed genes were annotated using DAVID software for Gene Ontology and GO Metacore for pathways analyses. Several enriched genes at each stage were validated using RT-qPCR. The enriched pathways involved were energy metabolism, cell cycle, immune response and fatty acid metabolism. Elucidation of these changes provides a greater understanding of molecular alterations after piezo-assisted biopsy. 2015 Thesis https://ir.uitm.edu.my/id/eprint/15717/ https://ir.uitm.edu.my/id/eprint/15717/1/TM_NORHAZLIN%20JUSOH%20%40%20MOHD%20YUSOFF%20MD%2015_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Medicine
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Radiographic magnification The embryo and fetus
spellingShingle	Radiographic magnification The embryo and fetus Jusoh @ Mohd Yusoff, Norhazlin Gene expression of biopsied murine preimplantation embryos / Norhazlin Jusoh @ Mohd Yusoff
description	Biopsy of embryo is a prerequisite technique employed in Preimplantation Genetic Diagnosis (PGD). Removal of one or two intact blastomeres can be carried out without detriment to embryonic development. However, the effect of piezo-assisted biopsy on gene expression of embryos remains to be elucidated. This study was conducted to elucidate the effect of piezo-assisted biopsy on the 8-cell, morula and blastocyst stages on embryonic development, global gene expression and their functional pathways. Following zona breaching by piezo-drilling, single blastomeres were removed from in vz'vo-derived 8-cell ICR mouse embryos. Biopsied embryos were allowed to develop further in Ml6 medium, and collected for RNA extraction after 1 h (8-cell), 16 h (morula) and 42 h (blastocyst). cDNA were converted, amplified, labelled and hybridized on the GeneChip®. Microarray data were analysed using GeneSpring GX 12. Gene expression of non-biopsied and post-biopsied embryos at the 8-cell, morula and blastocyst stages were compared using the t-test with p<0.05, including fold change of >1.5 for each gene. Results showed a significant difference in blastocyst development after piezo-assisted biopsy. A total of 82 genes, 75 genes and 66 genes were significantly different at the 8-cell, morula and blastocyst stages respectively. The differentially expressed genes were annotated using DAVID software for Gene Ontology and GO Metacore for pathways analyses. Several enriched genes at each stage were validated using RT-qPCR. The enriched pathways involved were energy metabolism, cell cycle, immune response and fatty acid metabolism. Elucidation of these changes provides a greater understanding of molecular alterations after piezo-assisted biopsy.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Jusoh @ Mohd Yusoff, Norhazlin
author_facet	Jusoh @ Mohd Yusoff, Norhazlin
author_sort	Jusoh @ Mohd Yusoff, Norhazlin
title	Gene expression of biopsied murine preimplantation embryos / Norhazlin Jusoh @ Mohd Yusoff
title_short	Gene expression of biopsied murine preimplantation embryos / Norhazlin Jusoh @ Mohd Yusoff
title_full	Gene expression of biopsied murine preimplantation embryos / Norhazlin Jusoh @ Mohd Yusoff
title_fullStr	Gene expression of biopsied murine preimplantation embryos / Norhazlin Jusoh @ Mohd Yusoff
title_full_unstemmed	Gene expression of biopsied murine preimplantation embryos / Norhazlin Jusoh @ Mohd Yusoff
title_sort	gene expression of biopsied murine preimplantation embryos / norhazlin jusoh @ mohd yusoff
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Medicine
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/15717/1/TM_NORHAZLIN%20JUSOH%20%40%20MOHD%20YUSOFF%20MD%2015_5.pdf
_version_	1783733445951750144

Gene expression of biopsied murine preimplantation embryos / Norhazlin Jusoh @ Mohd Yusoff

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