Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni

Palm Puree (PP) is a value-added product prepared from squeezed fruitlets of oil palm fresh fruit bunches. Fiber was incorporated at different percentages for samples T24 and PT99 at 2% and 5% fiber incorporated for each sample, respectively. The study was carried out to determine the potential nutr...

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Main Author:	Rosni, Nordalilah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Pharmacognosy Pharmaceutical substances (Plant, animal, and inorganic)
Online Access:	https://ir.uitm.edu.my/id/eprint/15907/1/TM_NORDALILAH%20ROSNI%20AS%2015_5.PDF
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spelling	my-uitm-ir.159072022-03-10T02:00:01Z Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni 2015 Rosni, Nordalilah Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) Palm Puree (PP) is a value-added product prepared from squeezed fruitlets of oil palm fresh fruit bunches. Fiber was incorporated at different percentages for samples T24 and PT99 at 2% and 5% fiber incorporated for each sample, respectively. The study was carried out to determine the potential nutritional benefits of PP such as neuroprotection, 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) inhibition and antimicrobial properties. Neuroprotection properties were observed in infant brain cancer; human neuroblastoma cell line SH-SY5Y differentiated by treatment of retinoic acid and induced with H2O2. The samples gave negative neurotoxicity results and promising neuroprotective effects on 80% confluent SHSY5Y cell line in 96 well plates at a dose dependent manner. PT99 displayed most protection against H2 0 2 -induced cell lines and followed by T24 at 100 (ig/mL (p<0.05). The HMGR inhibition assay aims to find the cholesterol lowering ability of PP using pravastatin, a known HMGR inhibitor as control. T24/5 gave a considerably better oxidation of NADPH at 0.159 Units/mgP, followed by PT99/5 (0.009 Units/mgP) as compared to pravastatin. Antimicrobial testing against selected pathogenic bacteria and fungi infecting human were performed using well diffusion method at concentrations of 200 (ig/ml, 300 (ig/ml, 500 (xg/ml, 800 fig/ml and 1 mg/ml. The test showed negative results as no inhibitory activities were observed. The HPLC analysis showed that the four vitamin E derivatives identified in all PP samples were a-T3, y-T3, 8-T3 and a-TP. T24 exhibited significantly (p<0.05) higher total amount of vitamin E as compared to PT99. In conclusion, PP can be used as a healthy value-added product for human consumption. 2015 Thesis https://ir.uitm.edu.my/id/eprint/15907/ https://ir.uitm.edu.my/id/eprint/15907/1/TM_NORDALILAH%20ROSNI%20AS%2015_5.PDF text en public mphil masters Universiti Teknologi MARA Faculty of Applied Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Pharmacognosy Pharmaceutical substances (Plant, animal, and inorganic)
spellingShingle	Pharmacognosy Pharmaceutical substances (Plant, animal, and inorganic) Rosni, Nordalilah Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni
description	Palm Puree (PP) is a value-added product prepared from squeezed fruitlets of oil palm fresh fruit bunches. Fiber was incorporated at different percentages for samples T24 and PT99 at 2% and 5% fiber incorporated for each sample, respectively. The study was carried out to determine the potential nutritional benefits of PP such as neuroprotection, 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) inhibition and antimicrobial properties. Neuroprotection properties were observed in infant brain cancer; human neuroblastoma cell line SH-SY5Y differentiated by treatment of retinoic acid and induced with H2O2. The samples gave negative neurotoxicity results and promising neuroprotective effects on 80% confluent SHSY5Y cell line in 96 well plates at a dose dependent manner. PT99 displayed most protection against H2 0 2 -induced cell lines and followed by T24 at 100 (ig/mL (p<0.05). The HMGR inhibition assay aims to find the cholesterol lowering ability of PP using pravastatin, a known HMGR inhibitor as control. T24/5 gave a considerably better oxidation of NADPH at 0.159 Units/mgP, followed by PT99/5 (0.009 Units/mgP) as compared to pravastatin. Antimicrobial testing against selected pathogenic bacteria and fungi infecting human were performed using well diffusion method at concentrations of 200 (ig/ml, 300 (ig/ml, 500 (xg/ml, 800 fig/ml and 1 mg/ml. The test showed negative results as no inhibitory activities were observed. The HPLC analysis showed that the four vitamin E derivatives identified in all PP samples were a-T3, y-T3, 8-T3 and a-TP. T24 exhibited significantly (p<0.05) higher total amount of vitamin E as compared to PT99. In conclusion, PP can be used as a healthy value-added product for human consumption.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Rosni, Nordalilah
author_facet	Rosni, Nordalilah
author_sort	Rosni, Nordalilah
title	Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni
title_short	Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni
title_full	Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni
title_fullStr	Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni
title_full_unstemmed	Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni
title_sort	neuroprotection, hmgr inhibition, vitamin e quantification and antimicrobial properties of palm puree / nordalilah rosni
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/15907/1/TM_NORDALILAH%20ROSNI%20AS%2015_5.PDF
_version_	1783733465868402688

Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni

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