Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah

Oil palm fronds (OPF) are cheap, widespread and largely available throughout the year. OPF contains rich hemicellulose which can be hydrolysed by enzyme for production of xylooligosaccharides (XOs). The aim of this study was to produce high yield of XOs from OPF hemicellulose. XOs production was per...

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Main Author:	Mohamad Damanhuri Shah, Siti Normah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Biochemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/15968/1/TM_SITI%20NORMAH%20MOHD%20DAMANHURI%20SHAH%20AS%2015_5.pdf
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id	my-uitm-ir.15968
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spelling	my-uitm-ir.159682022-03-10T02:20:43Z Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah 2015 Mohamad Damanhuri Shah, Siti Normah Biochemistry Oil palm fronds (OPF) are cheap, widespread and largely available throughout the year. OPF contains rich hemicellulose which can be hydrolysed by enzyme for production of xylooligosaccharides (XOs). The aim of this study was to produce high yield of XOs from OPF hemicellulose. XOs production was performed by enzymatic hydrolysis of hemicellulose which was obtained by alkaline extraction from the OPF. OPF hemicellulose was hydrolysed using an endo-xylanse from Trichoderma longibrachiatum and the effects of different pH, temperature, enzyme and substrate concentration and hydrolysis time on the XOs yield were investigated. The hydrolysate containing XOs was then fractionated by ultrafiltration using 10 kDa, 3 kDa and 1 kDa molecular weight cut off (MWCO) membrane to purify the crude of XOs. The maximum yield of XOs (46%) was obtained at pH 4.6, temperature of 4°C, enzyme concentration of 2 U/ml and substrate concentration of 2% for 8 hours hydrolysis time with a considerable amount of xylose (12.55%). The percent recoveries of XOs in the final permeate of 3 kDa and 1 kDa MWCO membrane were 78.32% and 76.18%, respectively. The results showed that 3 kDa MWCO membrane fractionated XOs better than 1 kDa MWCO membrane with low amount of monosaccharides and high yield of xylobiose (X2) and xylotriose (X3). The results indicated the potential utilization of OPF for the production of XOs by enzymatic hydrolysis, which could be further extended as functional food ingredients. 2015 Thesis https://ir.uitm.edu.my/id/eprint/15968/ https://ir.uitm.edu.my/id/eprint/15968/1/TM_SITI%20NORMAH%20MOHD%20DAMANHURI%20SHAH%20AS%2015_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Applied Science
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Biochemistry
spellingShingle	Biochemistry Mohamad Damanhuri Shah, Siti Normah Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah
description	Oil palm fronds (OPF) are cheap, widespread and largely available throughout the year. OPF contains rich hemicellulose which can be hydrolysed by enzyme for production of xylooligosaccharides (XOs). The aim of this study was to produce high yield of XOs from OPF hemicellulose. XOs production was performed by enzymatic hydrolysis of hemicellulose which was obtained by alkaline extraction from the OPF. OPF hemicellulose was hydrolysed using an endo-xylanse from Trichoderma longibrachiatum and the effects of different pH, temperature, enzyme and substrate concentration and hydrolysis time on the XOs yield were investigated. The hydrolysate containing XOs was then fractionated by ultrafiltration using 10 kDa, 3 kDa and 1 kDa molecular weight cut off (MWCO) membrane to purify the crude of XOs. The maximum yield of XOs (46%) was obtained at pH 4.6, temperature of 4°C, enzyme concentration of 2 U/ml and substrate concentration of 2% for 8 hours hydrolysis time with a considerable amount of xylose (12.55%). The percent recoveries of XOs in the final permeate of 3 kDa and 1 kDa MWCO membrane were 78.32% and 76.18%, respectively. The results showed that 3 kDa MWCO membrane fractionated XOs better than 1 kDa MWCO membrane with low amount of monosaccharides and high yield of xylobiose (X2) and xylotriose (X3). The results indicated the potential utilization of OPF for the production of XOs by enzymatic hydrolysis, which could be further extended as functional food ingredients.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Mohamad Damanhuri Shah, Siti Normah
author_facet	Mohamad Damanhuri Shah, Siti Normah
author_sort	Mohamad Damanhuri Shah, Siti Normah
title	Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah
title_short	Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah
title_full	Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah
title_fullStr	Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah
title_full_unstemmed	Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah
title_sort	production of xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / siti normah mohd damanhuri shah
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Science
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/15968/1/TM_SITI%20NORMAH%20MOHD%20DAMANHURI%20SHAH%20AS%2015_5.pdf
_version_	1783733470668783616

Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah

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