The influence of Tocotrienol on the development of preimplantation embryos and pregnancy outcome in corticosterone-treated mice / Nasibah Azme

Tocotrienol (TCT), a component of vitamin E is a powerful antioxidant whereas corticosterone (CORT), a known prooxidant has been shown to impair the development of embryo and pregnancy outcome. This study aims to determine the effect of TCT supplementation on the quality and in vitro development of...

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Main Author:	Azme, Nasibah
Format:	Thesis
Language:	English
Published:	2013
Online Access:	https://ir.uitm.edu.my/id/eprint/16351/1/TM_NASIBAH%20AZME%20MD%2013_5.pdf
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id	my-uitm-ir.16351
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spelling	my-uitm-ir.163512022-06-16T13:19:25Z The influence of Tocotrienol on the development of preimplantation embryos and pregnancy outcome in corticosterone-treated mice / Nasibah Azme 2013 Azme, Nasibah Tocotrienol (TCT), a component of vitamin E is a powerful antioxidant whereas corticosterone (CORT), a known prooxidant has been shown to impair the development of embryo and pregnancy outcome. This study aims to determine the effect of TCT supplementation on the quality and in vitro development of embryos and the pregnancy outcome in CORT-treated mice. In embryonic experiment, 5- to 6 weeks old female mice were divided into four groups of eight animals. They received CORT (10 mg/kg) intraperitoneal (ip) concurrently with TCT at the dose of 30, 60 and 90 mg/kg orally and the control group had 0.1 ml com oil (ip) and orally for seven consecutive days. On Day 7, after superovulation and mating, animals were euthanized and embryos were flushed out from the fallopian tubes. The morphology and in vitro development of embryos were recorded. In pregnancy outcome experiment, 7- to 8 weeks old female mice were divided into similar groups and treatment for the first seven days of pregnancy were conducted as above with using TCT at 60, 90 and 120 mg/kg. On Day 7 of pregnancy, laparotomy was done to determine the number of implantation sites; to observe any resorption signs while litter sizes were measured at birth. It was found that TCT (90 mg/kg) improved the quality of embryo whereas TCT (60 mg/kg) increased the number of embryos that reached hatched blastocyst stage in CORT-treated groups. In addition to that, TCT (60 mg/kg) increased the implantation sites, whereas TCT (120 mg/kg) decreased the resorption percentage and increased the level of progesterone hormone towards control in CORT-treated pregnant mice. Tocotrienol supplementation in our study is able to reverse the CORT-induced adverse impact on all reproductive outcomes as mentioned above. The effect of TCT alludes further exploration to ascertain their mechanism. 2013 Thesis https://ir.uitm.edu.my/id/eprint/16351/ https://ir.uitm.edu.my/id/eprint/16351/1/TM_NASIBAH%20AZME%20MD%2013_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Medicine
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
description	Tocotrienol (TCT), a component of vitamin E is a powerful antioxidant whereas corticosterone (CORT), a known prooxidant has been shown to impair the development of embryo and pregnancy outcome. This study aims to determine the effect of TCT supplementation on the quality and in vitro development of embryos and the pregnancy outcome in CORT-treated mice. In embryonic experiment, 5- to 6 weeks old female mice were divided into four groups of eight animals. They received CORT (10 mg/kg) intraperitoneal (ip) concurrently with TCT at the dose of 30, 60 and 90 mg/kg orally and the control group had 0.1 ml com oil (ip) and orally for seven consecutive days. On Day 7, after superovulation and mating, animals were euthanized and embryos were flushed out from the fallopian tubes. The morphology and in vitro development of embryos were recorded. In pregnancy outcome experiment, 7- to 8 weeks old female mice were divided into similar groups and treatment for the first seven days of pregnancy were conducted as above with using TCT at 60, 90 and 120 mg/kg. On Day 7 of pregnancy, laparotomy was done to determine the number of implantation sites; to observe any resorption signs while litter sizes were measured at birth. It was found that TCT (90 mg/kg) improved the quality of embryo whereas TCT (60 mg/kg) increased the number of embryos that reached hatched blastocyst stage in CORT-treated groups. In addition to that, TCT (60 mg/kg) increased the implantation sites, whereas TCT (120 mg/kg) decreased the resorption percentage and increased the level of progesterone hormone towards control in CORT-treated pregnant mice. Tocotrienol supplementation in our study is able to reverse the CORT-induced adverse impact on all reproductive outcomes as mentioned above. The effect of TCT alludes further exploration to ascertain their mechanism.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Azme, Nasibah
spellingShingle	Azme, Nasibah The influence of Tocotrienol on the development of preimplantation embryos and pregnancy outcome in corticosterone-treated mice / Nasibah Azme
author_facet	Azme, Nasibah
author_sort	Azme, Nasibah
title	The influence of Tocotrienol on the development of preimplantation embryos and pregnancy outcome in corticosterone-treated mice / Nasibah Azme
title_short	The influence of Tocotrienol on the development of preimplantation embryos and pregnancy outcome in corticosterone-treated mice / Nasibah Azme
title_full	The influence of Tocotrienol on the development of preimplantation embryos and pregnancy outcome in corticosterone-treated mice / Nasibah Azme
title_fullStr	The influence of Tocotrienol on the development of preimplantation embryos and pregnancy outcome in corticosterone-treated mice / Nasibah Azme
title_full_unstemmed	The influence of Tocotrienol on the development of preimplantation embryos and pregnancy outcome in corticosterone-treated mice / Nasibah Azme
title_sort	influence of tocotrienol on the development of preimplantation embryos and pregnancy outcome in corticosterone-treated mice / nasibah azme
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Medicine
publishDate	2013
url	https://ir.uitm.edu.my/id/eprint/16351/1/TM_NASIBAH%20AZME%20MD%2013_5.pdf
_version_	1783733509348655104

The influence of Tocotrienol on the development of preimplantation embryos and pregnancy outcome in corticosterone-treated mice / Nasibah Azme

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