Modulation of the amyloidogenic pathway by a Novel β-secretase inhibitor (F70HAB16) from Malaysian endophyte cytospora rhizophorae, in murine models for alzheimer’s disease / Richard Muhammad Johari James

Modulation of the amyloidogenic pathway by a Novel β-secretase inhibitor (F70HAB16) from Malaysian endophyte cytospora rhizophorae, in murine models for alzheimer’s disease / Richard Muhammad Johari James

Alzheimer’s disease (AD) is the most common form of dementia. Until recently, AD is managed by relieving the cognitive symptoms without addressing one of the purportedly fundamental causes of the disease which is the formation of the amyloid plaques. The deposition and aggregation of β-amyloid are k...

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Main Author: Muhammad Johari James, Richard
Format: Thesis
Language:English
Published: 2014
Online Access:https://ir.uitm.edu.my/id/eprint/16480/1/ABS_RICHARD%20MUHAMMAD%20JOHARI%20JAMES%20TDRA%20VOL%205%20IGS_14.pdf
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spelling my-uitm-ir.164802022-03-23T03:10:53Z Modulation of the amyloidogenic pathway by a Novel β-secretase inhibitor (F70HAB16) from Malaysian endophyte cytospora rhizophorae, in murine models for alzheimer’s disease / Richard Muhammad Johari James 2014 Muhammad Johari James, Richard Alzheimer’s disease (AD) is the most common form of dementia. Until recently, AD is managed by relieving the cognitive symptoms without addressing one of the purportedly fundamental causes of the disease which is the formation of the amyloid plaques. The deposition and aggregation of β-amyloid are key events in the onset, progression and pathogenesis of AD. Thus one of the emerging strategies in treating AD is to inhibit the enzyme responsible for the formation of amyloid plaques, which is β -site amyloid cleaving enzyme (BACE-1). Endophytes are currently viewed as an outstanding source of bioactive natural products and may provide BACE-1 inhibitors as potential drug candidates for the treatment of AD. A novel bioactive compound, F70HAB16 was successfully isolated from a local endophytic strain and was found to inhibit the BACE-1 enzyme in vitro (IC₅₀=13 uM). Oral treatment with 5 mg/kg of F70HAB16 for 14 days in scopolamine-induced memory deficit mice model was found to restore memory impairment caused by scopolamine in the radial arm maze and Morris water maze (MWM) tasks. The same treatment was found to improve spatial memory and learning in MWM tasks in a transgenic mice model of AD (B6.129TG) carrying the human APP-Swedish mutation (K670N/M671L). Analysis of the blood plasma and brain tissue of the transgenic mice revealed that the expression of amyloidogenic proteins decreased following treatment with the BACE- 1 inhibitor. Oxidative stress may play a significant but yet undefined role in the development of AD. It was found that the administration of 5 mg/kg of the F70HAB16 reduced the lipid peroxidation index and restored the antioxidant activities of catalase, superoxide dismutase, glutathione reductase and glutathione in the brain tissue of the scopolamine-induced mice model. The treatment with 5 mg/kg of F70HAB16 also redressed the level of nitric oxide in the scopolamine-induced mice. Hence F70HAB16 may prove to be beneficial in the treatment of AD by alleviating the oxidative stress associated with this disease. Furthermore, F70HAB16 also demonstrated neuroprotective properties on the cholinergic system of the scopolamine-induced mice. Finally, metabolomics study of the blood plasma revealed that F70HAB16 down-regulated sphingolipids such as dihydrosphingosine, phytosphingosine and C16-sphinganin. These metabolites were recently proposed as biomarkers for AD since they were found to be up-regulated in the blood plasma of AD patients. 2014 Thesis https://ir.uitm.edu.my/id/eprint/16480/ https://ir.uitm.edu.my/id/eprint/16480/1/ABS_RICHARD%20MUHAMMAD%20JOHARI%20JAMES%20TDRA%20VOL%205%20IGS_14.pdf text en public phd doctoral Universiti Teknologi MARA Faculty of Pharmacy
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
description Alzheimer’s disease (AD) is the most common form of dementia. Until recently, AD is managed by relieving the cognitive symptoms without addressing one of the purportedly fundamental causes of the disease which is the formation of the amyloid plaques. The deposition and aggregation of β-amyloid are key events in the onset, progression and pathogenesis of AD. Thus one of the emerging strategies in treating AD is to inhibit the enzyme responsible for the formation of amyloid plaques, which is β -site amyloid cleaving enzyme (BACE-1). Endophytes are currently viewed as an outstanding source of bioactive natural products and may provide BACE-1 inhibitors as potential drug candidates for the treatment of AD. A novel bioactive compound, F70HAB16 was successfully isolated from a local endophytic strain and was found to inhibit the BACE-1 enzyme in vitro (IC₅₀=13 uM). Oral treatment with 5 mg/kg of F70HAB16 for 14 days in scopolamine-induced memory deficit mice model was found to restore memory impairment caused by scopolamine in the radial arm maze and Morris water maze (MWM) tasks. The same treatment was found to improve spatial memory and learning in MWM tasks in a transgenic mice model of AD (B6.129TG) carrying the human APP-Swedish mutation (K670N/M671L). Analysis of the blood plasma and brain tissue of the transgenic mice revealed that the expression of amyloidogenic proteins decreased following treatment with the BACE- 1 inhibitor. Oxidative stress may play a significant but yet undefined role in the development of AD. It was found that the administration of 5 mg/kg of the F70HAB16 reduced the lipid peroxidation index and restored the antioxidant activities of catalase, superoxide dismutase, glutathione reductase and glutathione in the brain tissue of the scopolamine-induced mice model. The treatment with 5 mg/kg of F70HAB16 also redressed the level of nitric oxide in the scopolamine-induced mice. Hence F70HAB16 may prove to be beneficial in the treatment of AD by alleviating the oxidative stress associated with this disease. Furthermore, F70HAB16 also demonstrated neuroprotective properties on the cholinergic system of the scopolamine-induced mice. Finally, metabolomics study of the blood plasma revealed that F70HAB16 down-regulated sphingolipids such as dihydrosphingosine, phytosphingosine and C16-sphinganin. These metabolites were recently proposed as biomarkers for AD since they were found to be up-regulated in the blood plasma of AD patients.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Muhammad Johari James, Richard
spellingShingle Muhammad Johari James, Richard
Modulation of the amyloidogenic pathway by a Novel β-secretase inhibitor (F70HAB16) from Malaysian endophyte cytospora rhizophorae, in murine models for alzheimer’s disease / Richard Muhammad Johari James
author_facet Muhammad Johari James, Richard
author_sort Muhammad Johari James, Richard
title Modulation of the amyloidogenic pathway by a Novel β-secretase inhibitor (F70HAB16) from Malaysian endophyte cytospora rhizophorae, in murine models for alzheimer’s disease / Richard Muhammad Johari James
title_short Modulation of the amyloidogenic pathway by a Novel β-secretase inhibitor (F70HAB16) from Malaysian endophyte cytospora rhizophorae, in murine models for alzheimer’s disease / Richard Muhammad Johari James
title_full Modulation of the amyloidogenic pathway by a Novel β-secretase inhibitor (F70HAB16) from Malaysian endophyte cytospora rhizophorae, in murine models for alzheimer’s disease / Richard Muhammad Johari James
title_fullStr Modulation of the amyloidogenic pathway by a Novel β-secretase inhibitor (F70HAB16) from Malaysian endophyte cytospora rhizophorae, in murine models for alzheimer’s disease / Richard Muhammad Johari James
title_full_unstemmed Modulation of the amyloidogenic pathway by a Novel β-secretase inhibitor (F70HAB16) from Malaysian endophyte cytospora rhizophorae, in murine models for alzheimer’s disease / Richard Muhammad Johari James
title_sort modulation of the amyloidogenic pathway by a novel β-secretase inhibitor (f70hab16) from malaysian endophyte cytospora rhizophorae, in murine models for alzheimer’s disease / richard muhammad johari james
granting_institution Universiti Teknologi MARA
granting_department Faculty of Pharmacy
publishDate 2014
url https://ir.uitm.edu.my/id/eprint/16480/1/ABS_RICHARD%20MUHAMMAD%20JOHARI%20JAMES%20TDRA%20VOL%205%20IGS_14.pdf
_version_ 1783733536743751680

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