Intracellular calcium release during in vitro mammalian embryonic development in the presence of Kalanchoe Pinnata extract / Nor Azila Md Ludin

Intracellular calcium release during in vitro mammalian embryonic development in the presence of Kalanchoe Pinnata extract / Nor Azila Md Ludin

Medium composition plays an important role in mammalian in vitro culture. The availability of commercial culture media with different formulations and chemical constituents indicates the specific need and application of using medium for in vitro culture. WM was selected in this study for rabbit and...

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Main Author: Md Ludin, Nor Azila
Format: Thesis
Language:English
Published: 2014
Online Access:https://ir.uitm.edu.my/id/eprint/17438/2/TM_NOR%20AZILA%20MD%20LUDIN%20AS%2014_5.pdf
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spelling my-uitm-ir.174382022-03-23T02:22:46Z Intracellular calcium release during in vitro mammalian embryonic development in the presence of Kalanchoe Pinnata extract / Nor Azila Md Ludin 2014 Md Ludin, Nor Azila Medium composition plays an important role in mammalian in vitro culture. The availability of commercial culture media with different formulations and chemical constituents indicates the specific need and application of using medium for in vitro culture. WM was selected in this study for rabbit and mouse embryo cultures. WM+1 mg/ml and WM+2.5 mg/ml of K. pinnata extract were used to investigate the efficacy of the extract on intracellular calcium release in relation to cleavage development of embryos. The embryos were cultured in the respective medium and the cleavage developments were observed daily for six consecutive days. The intracellular calcium release was measured from the 2- to 6/8-cell embryos and fluorescence imaging of nuclear materials, microtubules and mitochondria were obtained. Rabbit embryos cultured in WM, WM+1 mg/ml and WM+2.5 mg/ml of K. pinnata extract had developed to morula stage with 6.35 ± 6.32%, 4.44 ± 4.17% and 5.13 ± 2.92%, respectively. There was a significantly higher percentage of development at 3/4-cell stage (p<0.05) in WM+2.5 mg/ml of K. pinnata extract. The intracellular calcium release in 3/4-cell and 6/8-cell stages were in the range of 3.95-50.10 \xM and 24.50- 782.00 |iM, respectively. Embryos retrieved at 48 hours post hCG survived until blastocyst (0.84 ± 0.47%), 8-cell (2.01 ± 0.97%) and morula stage (2.20 ± 1.17%) in WM, WM+1 mg/ml and WM+2.5 mg/ml of K. pinnata extract, respectively. Low intracellular calcium release caused fragmented nuclei and the mitochondria and microtubules were aggregated only at the center part of blastomeres. The presence of K. pinnata extract as supplement for the culture medium had significantly enhanced the development of rabbit embryos at 3/4-cell stage and mouse embryos at 2-cell and 8-cell stage (p<0.05). Different formulations of herbal extract as supplement are required to improve the embryo development at each cell stage. 2014 Thesis https://ir.uitm.edu.my/id/eprint/17438/ https://ir.uitm.edu.my/id/eprint/17438/2/TM_NOR%20AZILA%20MD%20LUDIN%20AS%2014_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Applied Sciences
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
description Medium composition plays an important role in mammalian in vitro culture. The availability of commercial culture media with different formulations and chemical constituents indicates the specific need and application of using medium for in vitro culture. WM was selected in this study for rabbit and mouse embryo cultures. WM+1 mg/ml and WM+2.5 mg/ml of K. pinnata extract were used to investigate the efficacy of the extract on intracellular calcium release in relation to cleavage development of embryos. The embryos were cultured in the respective medium and the cleavage developments were observed daily for six consecutive days. The intracellular calcium release was measured from the 2- to 6/8-cell embryos and fluorescence imaging of nuclear materials, microtubules and mitochondria were obtained. Rabbit embryos cultured in WM, WM+1 mg/ml and WM+2.5 mg/ml of K. pinnata extract had developed to morula stage with 6.35 ± 6.32%, 4.44 ± 4.17% and 5.13 ± 2.92%, respectively. There was a significantly higher percentage of development at 3/4-cell stage (p<0.05) in WM+2.5 mg/ml of K. pinnata extract. The intracellular calcium release in 3/4-cell and 6/8-cell stages were in the range of 3.95-50.10 \xM and 24.50- 782.00 |iM, respectively. Embryos retrieved at 48 hours post hCG survived until blastocyst (0.84 ± 0.47%), 8-cell (2.01 ± 0.97%) and morula stage (2.20 ± 1.17%) in WM, WM+1 mg/ml and WM+2.5 mg/ml of K. pinnata extract, respectively. Low intracellular calcium release caused fragmented nuclei and the mitochondria and microtubules were aggregated only at the center part of blastomeres. The presence of K. pinnata extract as supplement for the culture medium had significantly enhanced the development of rabbit embryos at 3/4-cell stage and mouse embryos at 2-cell and 8-cell stage (p<0.05). Different formulations of herbal extract as supplement are required to improve the embryo development at each cell stage.
format Thesis
qualification_name Master of Philosophy (M.Phil.)
qualification_level Master's degree
author Md Ludin, Nor Azila
spellingShingle Md Ludin, Nor Azila
Intracellular calcium release during in vitro mammalian embryonic development in the presence of Kalanchoe Pinnata extract / Nor Azila Md Ludin
author_facet Md Ludin, Nor Azila
author_sort Md Ludin, Nor Azila
title Intracellular calcium release during in vitro mammalian embryonic development in the presence of Kalanchoe Pinnata extract / Nor Azila Md Ludin
title_short Intracellular calcium release during in vitro mammalian embryonic development in the presence of Kalanchoe Pinnata extract / Nor Azila Md Ludin
title_full Intracellular calcium release during in vitro mammalian embryonic development in the presence of Kalanchoe Pinnata extract / Nor Azila Md Ludin
title_fullStr Intracellular calcium release during in vitro mammalian embryonic development in the presence of Kalanchoe Pinnata extract / Nor Azila Md Ludin
title_full_unstemmed Intracellular calcium release during in vitro mammalian embryonic development in the presence of Kalanchoe Pinnata extract / Nor Azila Md Ludin
title_sort intracellular calcium release during in vitro mammalian embryonic development in the presence of kalanchoe pinnata extract / nor azila md ludin
granting_institution Universiti Teknologi MARA
granting_department Faculty of Applied Sciences
publishDate 2014
url https://ir.uitm.edu.my/id/eprint/17438/2/TM_NOR%20AZILA%20MD%20LUDIN%20AS%2014_5.pdf
_version_ 1783733562331103232

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