The comparison between betaglucuronidase (BG) with plasma butyrylcholinesterase (BChE) as biomarker of low-level organophosphate insecticide among vector control operators / Muhamad Ashraf Rostam

The comparison between betaglucuronidase (BG) with plasma butyrylcholinesterase (BChE) as biomarker of low-level organophosphate insecticide among vector control operators / Muhamad Ashraf Rostam

This study aims to compare two detection methods of organophosphate poisoning using plasma butyrylcholinesterase (BChE) and β-glucuronidase (BG) assay, their relationship with insecticide exposure characteristics, as well as comparing the differences of both biomarkers between the exposed group (vec...

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Main Author: Rostam, Muhamad Ashraf
Format: Thesis
Language:English
Published: 2012
Online Access:https://ir.uitm.edu.my/id/eprint/17658/2/TM_MUHAMAD%20ASHRAF%20ROSTAM%20HS%2012_5.pdf
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spelling my-uitm-ir.176582022-04-21T03:18:40Z The comparison between betaglucuronidase (BG) with plasma butyrylcholinesterase (BChE) as biomarker of low-level organophosphate insecticide among vector control operators / Muhamad Ashraf Rostam 2012-04 Rostam, Muhamad Ashraf This study aims to compare two detection methods of organophosphate poisoning using plasma butyrylcholinesterase (BChE) and β-glucuronidase (BG) assay, their relationship with insecticide exposure characteristics, as well as comparing the differences of both biomarkers between the exposed group (vector control operators) with the non-exposed control group (normal Malaysian). A total of 96 vector control operators from four centres; Pejabat Kesihatan Daerah (PKD) Petaling, PKD Kuala Langat, PKD Kuala Selangor and Majlis Bandaraya Shah Alam were the subject group while 49 healthy blood donors from Pusat Darah Negara were recruited as the negative control group. Occupational insecticide poisoning characteristics of the subject group (employment period, lapse time between insecticide use and blood collection, type of insecticide used, types of Personal Protective Equipments (PPE) and compliance with use) were obtained through questionnaire. The association of BChE and BG level with age, race, employment year, compliance to PPE use, and lapse time between insecticide use and blood collection were evaluated. Both assays showed similar results where both were not influenced by the mentioned variables. A comparison between the methods of both the assays provides insight into suitability of each biomarker for different situations. BChE assay is recommended as a suitable assay for acute exposure to insecticides due to its rapid test turnaround time. Meanwhile BG assay is suitable for continuous biomonitoring of organophosphate poisoning among occupationally exposed workers due to its low cost and minimal laboratory requirement. A correlation test between BChE and BG level showed significant positive correlation (r=0.301, p=0.003). Comparison of the results of BChE and BG level among the control and subject group showed no significant difference (/?>0.05). In conclusion, findings of this study show that assaying for the BG enzyme can be an alternative or additional method to determine organophosphate poisoning alongside the BChE assay. The reference value established for BG assay from this study is between the range of 40- 720 U/dL and mean of 204.19±136.88 U/dL. Results of both assays had also shown that vector control operators in these four areas in Selangor are not exposed to harmful level of organophosphate. 2012-04 Thesis https://ir.uitm.edu.my/id/eprint/17658/ https://ir.uitm.edu.my/id/eprint/17658/2/TM_MUHAMAD%20ASHRAF%20ROSTAM%20HS%2012_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Health Sciences Hassan (Hj), Zuridah (Assoc. Prof. Dr Hjh)
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Hassan (Hj), Zuridah (Assoc. Prof. Dr Hjh)
description This study aims to compare two detection methods of organophosphate poisoning using plasma butyrylcholinesterase (BChE) and β-glucuronidase (BG) assay, their relationship with insecticide exposure characteristics, as well as comparing the differences of both biomarkers between the exposed group (vector control operators) with the non-exposed control group (normal Malaysian). A total of 96 vector control operators from four centres; Pejabat Kesihatan Daerah (PKD) Petaling, PKD Kuala Langat, PKD Kuala Selangor and Majlis Bandaraya Shah Alam were the subject group while 49 healthy blood donors from Pusat Darah Negara were recruited as the negative control group. Occupational insecticide poisoning characteristics of the subject group (employment period, lapse time between insecticide use and blood collection, type of insecticide used, types of Personal Protective Equipments (PPE) and compliance with use) were obtained through questionnaire. The association of BChE and BG level with age, race, employment year, compliance to PPE use, and lapse time between insecticide use and blood collection were evaluated. Both assays showed similar results where both were not influenced by the mentioned variables. A comparison between the methods of both the assays provides insight into suitability of each biomarker for different situations. BChE assay is recommended as a suitable assay for acute exposure to insecticides due to its rapid test turnaround time. Meanwhile BG assay is suitable for continuous biomonitoring of organophosphate poisoning among occupationally exposed workers due to its low cost and minimal laboratory requirement. A correlation test between BChE and BG level showed significant positive correlation (r=0.301, p=0.003). Comparison of the results of BChE and BG level among the control and subject group showed no significant difference (/?>0.05). In conclusion, findings of this study show that assaying for the BG enzyme can be an alternative or additional method to determine organophosphate poisoning alongside the BChE assay. The reference value established for BG assay from this study is between the range of 40- 720 U/dL and mean of 204.19±136.88 U/dL. Results of both assays had also shown that vector control operators in these four areas in Selangor are not exposed to harmful level of organophosphate.
format Thesis
qualification_name Master of Philosophy (M.Phil.)
qualification_level Master's degree
author Rostam, Muhamad Ashraf
spellingShingle Rostam, Muhamad Ashraf
The comparison between betaglucuronidase (BG) with plasma butyrylcholinesterase (BChE) as biomarker of low-level organophosphate insecticide among vector control operators / Muhamad Ashraf Rostam
author_facet Rostam, Muhamad Ashraf
author_sort Rostam, Muhamad Ashraf
title The comparison between betaglucuronidase (BG) with plasma butyrylcholinesterase (BChE) as biomarker of low-level organophosphate insecticide among vector control operators / Muhamad Ashraf Rostam
title_short The comparison between betaglucuronidase (BG) with plasma butyrylcholinesterase (BChE) as biomarker of low-level organophosphate insecticide among vector control operators / Muhamad Ashraf Rostam
title_full The comparison between betaglucuronidase (BG) with plasma butyrylcholinesterase (BChE) as biomarker of low-level organophosphate insecticide among vector control operators / Muhamad Ashraf Rostam
title_fullStr The comparison between betaglucuronidase (BG) with plasma butyrylcholinesterase (BChE) as biomarker of low-level organophosphate insecticide among vector control operators / Muhamad Ashraf Rostam
title_full_unstemmed The comparison between betaglucuronidase (BG) with plasma butyrylcholinesterase (BChE) as biomarker of low-level organophosphate insecticide among vector control operators / Muhamad Ashraf Rostam
title_sort comparison between betaglucuronidase (bg) with plasma butyrylcholinesterase (bche) as biomarker of low-level organophosphate insecticide among vector control operators / muhamad ashraf rostam
granting_institution Universiti Teknologi MARA
granting_department Faculty of Health Sciences
publishDate 2012
url https://ir.uitm.edu.my/id/eprint/17658/2/TM_MUHAMAD%20ASHRAF%20ROSTAM%20HS%2012_5.pdf
_version_ 1783733573525700608

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