Optimization of a cryopreservation protocol for a Malaysian fresh water micro algal, Chlorella vulgaris / Nor Afiqah Anua

Optimization of a cryopreservation protocol for a Malaysian fresh water micro algal, Chlorella vulgaris / Nor Afiqah Anua

Microalgae is important in many biotechnological exploitations in producing valuable products, services and processes. Many microalgal strains are able to grow post cryopreservation because the technology of cryopreservation has improved tremendously over the years. Cryopreservation is a process whe...

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Format: Thesis
Language:English
Published: 2016
Online Access:https://ir.uitm.edu.my/id/eprint/17865/2/TM_NOR%20AFIQAH%20ANUA%20AS%2016_5.pdf
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spelling my-uitm-ir.178652019-03-12T07:57:20Z Optimization of a cryopreservation protocol for a Malaysian fresh water micro algal, Chlorella vulgaris / Nor Afiqah Anua 2016 Microalgae is important in many biotechnological exploitations in producing valuable products, services and processes. Many microalgal strains are able to grow post cryopreservation because the technology of cryopreservation has improved tremendously over the years. Cryopreservation is a process whereby the cells or the whole tissue are preserved by cooling to an ultralow temperature of -196°C such that it remains capable of growth upon thawing. In this study, Chlorella vulgaris was used to determine the viability post cryopreservation at different concentrations of cryoprotectant employing three different protocols. Dimethyl sulfoxide (DMSO) at different concentrations of 0%, 10%, 20% and 30% were added to the microalgal suspension in the research. Protocol 1 involves direct plunging into liquid nitrogen, Protocol 2 is slow cooling to -80°C and Protocol 3 is slow cooling to -20°C. The absorbance value at 540 nm was used as a measurement to determine the growth post cryopreservation. The absorbance value recorded with 30% DMSO in Protocol 2 showed the highest at 0.387 ± 0.015 and was significant at p<0.05 compared to Protocol 1 and 3. The best protocol for the optimization of the cryopreservation of C. vulgaris was Protocol 2 using slow cooling to -80 °C treated with 30% DMSO as the cryoprotective agent. 2016 Thesis https://ir.uitm.edu.my/id/eprint/17865/ https://ir.uitm.edu.my/id/eprint/17865/2/TM_NOR%20AFIQAH%20ANUA%20AS%2016_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Applied Sciences
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
description Microalgae is important in many biotechnological exploitations in producing valuable products, services and processes. Many microalgal strains are able to grow post cryopreservation because the technology of cryopreservation has improved tremendously over the years. Cryopreservation is a process whereby the cells or the whole tissue are preserved by cooling to an ultralow temperature of -196°C such that it remains capable of growth upon thawing. In this study, Chlorella vulgaris was used to determine the viability post cryopreservation at different concentrations of cryoprotectant employing three different protocols. Dimethyl sulfoxide (DMSO) at different concentrations of 0%, 10%, 20% and 30% were added to the microalgal suspension in the research. Protocol 1 involves direct plunging into liquid nitrogen, Protocol 2 is slow cooling to -80°C and Protocol 3 is slow cooling to -20°C. The absorbance value at 540 nm was used as a measurement to determine the growth post cryopreservation. The absorbance value recorded with 30% DMSO in Protocol 2 showed the highest at 0.387 ± 0.015 and was significant at p<0.05 compared to Protocol 1 and 3. The best protocol for the optimization of the cryopreservation of C. vulgaris was Protocol 2 using slow cooling to -80 °C treated with 30% DMSO as the cryoprotective agent.
format Thesis
qualification_name Master of Philosophy (M.Phil.)
qualification_level Master's degree
title Optimization of a cryopreservation protocol for a Malaysian fresh water micro algal, Chlorella vulgaris / Nor Afiqah Anua
spellingShingle Optimization of a cryopreservation protocol for a Malaysian fresh water micro algal, Chlorella vulgaris / Nor Afiqah Anua
title_short Optimization of a cryopreservation protocol for a Malaysian fresh water micro algal, Chlorella vulgaris / Nor Afiqah Anua
title_full Optimization of a cryopreservation protocol for a Malaysian fresh water micro algal, Chlorella vulgaris / Nor Afiqah Anua
title_fullStr Optimization of a cryopreservation protocol for a Malaysian fresh water micro algal, Chlorella vulgaris / Nor Afiqah Anua
title_full_unstemmed Optimization of a cryopreservation protocol for a Malaysian fresh water micro algal, Chlorella vulgaris / Nor Afiqah Anua
title_sort optimization of a cryopreservation protocol for a malaysian fresh water micro algal, chlorella vulgaris / nor afiqah anua
granting_institution Universiti Teknologi MARA
granting_department Faculty of Applied Sciences
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/17865/2/TM_NOR%20AFIQAH%20ANUA%20AS%2016_5.pdf
_version_ 1783733613797310464

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