Molecular sexing approach using CHD marker for monomorphic birds of Gunung Ledang, National Park, Johor / Jessey Angat

Many conservation centers in Malaysia have presented serious problem in sexing monomorphic birds as it does not possess any noticeable morphological differences between sexes. Therefore, it is very hard for them to establish the breeding strategies, conservation and management programs. Polymerase C...

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Main Author:	Jessey Angat
Format:	Thesis
Language:	English
Published:	2016
Online Access:	https://ir.uitm.edu.my/id/eprint/17872/2/TM_JESSEY%20ANGAT%20AS%2016_5.pdf
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spelling	my-uitm-ir.178722019-03-12T08:01:10Z Molecular sexing approach using CHD marker for monomorphic birds of Gunung Ledang, National Park, Johor / Jessey Angat 2016 Jessey Angat Many conservation centers in Malaysia have presented serious problem in sexing monomorphic birds as it does not possess any noticeable morphological differences between sexes. Therefore, it is very hard for them to establish the breeding strategies, conservation and management programs. Polymerase Chain Reaction (PCR) would accurately recognize which gender, by comparing the intron length between the Chromo Helicase DNA-binding gene (CHD) in Z and W chromosomes. A total of 150 individual birds from 5 species were captured from 10 sites by mist nets. The genes were amplified with 1237L/1272H, 2550F/2718R and P2/P8 primer pairs. Result showed sex determination was unambiguous in all species thus, PCR method alone was sufficient for an effective and fast in sex determination except for the Water-breasted Waterhen with 2550F/2718R primer set. The sex ratio between male and female individual was 1:1. The validity and effectiveness of using thoracic feather were tested with the aim to inflict the only potential feather that will be used for future sexing purposes. Adding more samples is vital in the future as to gain more accurate number of individual species in Gunung Ledang, National Park, Johor. In addition, habitat preferences and behavior as well as morphometric measurement in each individual of bird is required as this would assist in the alternative sexing methodology notably using discriminant function along with PCR-based molecular techniques. Overall, the outcome could have a crucial impact on many protection and reintroduction activities based on molecular-based application, hence granting preservation and enhancement of biodiversity in Malaysia. 2016 Thesis https://ir.uitm.edu.my/id/eprint/17872/ https://ir.uitm.edu.my/id/eprint/17872/2/TM_JESSEY%20ANGAT%20AS%2016_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Applied Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
description	Many conservation centers in Malaysia have presented serious problem in sexing monomorphic birds as it does not possess any noticeable morphological differences between sexes. Therefore, it is very hard for them to establish the breeding strategies, conservation and management programs. Polymerase Chain Reaction (PCR) would accurately recognize which gender, by comparing the intron length between the Chromo Helicase DNA-binding gene (CHD) in Z and W chromosomes. A total of 150 individual birds from 5 species were captured from 10 sites by mist nets. The genes were amplified with 1237L/1272H, 2550F/2718R and P2/P8 primer pairs. Result showed sex determination was unambiguous in all species thus, PCR method alone was sufficient for an effective and fast in sex determination except for the Water-breasted Waterhen with 2550F/2718R primer set. The sex ratio between male and female individual was 1:1. The validity and effectiveness of using thoracic feather were tested with the aim to inflict the only potential feather that will be used for future sexing purposes. Adding more samples is vital in the future as to gain more accurate number of individual species in Gunung Ledang, National Park, Johor. In addition, habitat preferences and behavior as well as morphometric measurement in each individual of bird is required as this would assist in the alternative sexing methodology notably using discriminant function along with PCR-based molecular techniques. Overall, the outcome could have a crucial impact on many protection and reintroduction activities based on molecular-based application, hence granting preservation and enhancement of biodiversity in Malaysia.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Jessey Angat
spellingShingle	Jessey Angat Molecular sexing approach using CHD marker for monomorphic birds of Gunung Ledang, National Park, Johor / Jessey Angat
author_facet	Jessey Angat
author_sort	Jessey Angat
title	Molecular sexing approach using CHD marker for monomorphic birds of Gunung Ledang, National Park, Johor / Jessey Angat
title_short	Molecular sexing approach using CHD marker for monomorphic birds of Gunung Ledang, National Park, Johor / Jessey Angat
title_full	Molecular sexing approach using CHD marker for monomorphic birds of Gunung Ledang, National Park, Johor / Jessey Angat
title_fullStr	Molecular sexing approach using CHD marker for monomorphic birds of Gunung Ledang, National Park, Johor / Jessey Angat
title_full_unstemmed	Molecular sexing approach using CHD marker for monomorphic birds of Gunung Ledang, National Park, Johor / Jessey Angat
title_sort	molecular sexing approach using chd marker for monomorphic birds of gunung ledang, national park, johor / jessey angat
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/17872/2/TM_JESSEY%20ANGAT%20AS%2016_5.pdf
_version_	1783733615244345344

Molecular sexing approach using CHD marker for monomorphic birds of Gunung Ledang, National Park, Johor / Jessey Angat

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