Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim

These present works have focus on the isolation of galactomannan and synthesis of galactomannan nanoparticles. Galactomannan nanoparticles possess attractive properties such as biodegradable and biocompatible; significant in biomedical applications. Galactomannan was isolated from the mature seeds o...

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Main Author:	Rahim, Nurnadiah
Format:	Thesis
Language:	English
Published:	2016
Online Access:	https://ir.uitm.edu.my/id/eprint/17875/2/17875.pdf
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id	my-uitm-ir.17875
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spelling	my-uitm-ir.178752024-07-24T06:20:11Z Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim 2016 Rahim, Nurnadiah These present works have focus on the isolation of galactomannan and synthesis of galactomannan nanoparticles. Galactomannan nanoparticles possess attractive properties such as biodegradable and biocompatible; significant in biomedical applications. Galactomannan was isolated from the mature seeds of a local plant, petai belalang known scientifically as Leucaena leucocephala via water extraction followed by precipitation with ethanol and purified by ion-exchange chromatography. It was chemically analyzed by UV, NMR, FTIR, GPC and HPLC. Pure galactomannan was successfully isolated with a yield of 50.31 % (w/w). It consisted of D-mannose and Dgalactose with mannose: galactose (M/G) ratio of 1.01. The molecular weight of the galactomannan was found to be 64 kDa. Galactomannan obtained from this present work is soluble in water. Galactomannan were then modified to acrylated galactomannan (AcGA) which act as a precursor molecule for the preparation of crosslinked galactomannan nanoparticles (CL-GA). AcGA was synthesized using acryloyl chloride (AC) as a chemical modifier through esterification process. Trimethylolpropane triacrylate (TMPTA) was used as the crosslinker for the preparation of CL-GA. Successful conjugation of GA with acrylates was verified by ¹H NMR and FTIR spectroscopies. Thermal properties of pure galactomannan, AcGA and CL-GA were measured using DSC to complement spectroscopic results. TEM images revealed the particle size of AcGA to be in the range of 30-100 nm; and increased to 200-850 nm range after the crosslinking process. Efficiency of CL-GA to trap crystal violet dye as a model drug was followed by UV Vis Spectroscopy. Results showed that CL-GA is capable of trapping crystal violet dye and has the potential to be used as a drug carrier. 2016 Thesis https://ir.uitm.edu.my/id/eprint/17875/ https://ir.uitm.edu.my/id/eprint/17875/2/17875.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Applied Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
description	These present works have focus on the isolation of galactomannan and synthesis of galactomannan nanoparticles. Galactomannan nanoparticles possess attractive properties such as biodegradable and biocompatible; significant in biomedical applications. Galactomannan was isolated from the mature seeds of a local plant, petai belalang known scientifically as Leucaena leucocephala via water extraction followed by precipitation with ethanol and purified by ion-exchange chromatography. It was chemically analyzed by UV, NMR, FTIR, GPC and HPLC. Pure galactomannan was successfully isolated with a yield of 50.31 % (w/w). It consisted of D-mannose and Dgalactose with mannose: galactose (M/G) ratio of 1.01. The molecular weight of the galactomannan was found to be 64 kDa. Galactomannan obtained from this present work is soluble in water. Galactomannan were then modified to acrylated galactomannan (AcGA) which act as a precursor molecule for the preparation of crosslinked galactomannan nanoparticles (CL-GA). AcGA was synthesized using acryloyl chloride (AC) as a chemical modifier through esterification process. Trimethylolpropane triacrylate (TMPTA) was used as the crosslinker for the preparation of CL-GA. Successful conjugation of GA with acrylates was verified by ¹H NMR and FTIR spectroscopies. Thermal properties of pure galactomannan, AcGA and CL-GA were measured using DSC to complement spectroscopic results. TEM images revealed the particle size of AcGA to be in the range of 30-100 nm; and increased to 200-850 nm range after the crosslinking process. Efficiency of CL-GA to trap crystal violet dye as a model drug was followed by UV Vis Spectroscopy. Results showed that CL-GA is capable of trapping crystal violet dye and has the potential to be used as a drug carrier.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Rahim, Nurnadiah
spellingShingle	Rahim, Nurnadiah Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim
author_facet	Rahim, Nurnadiah
author_sort	Rahim, Nurnadiah
title	Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim
title_short	Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim
title_full	Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim
title_fullStr	Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim
title_full_unstemmed	Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim
title_sort	isolation and purification of galactomannan from leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / nurnadiah rahim
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/17875/2/17875.pdf
_version_	1811768598271623168

Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim

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