Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian

In the present work, isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura (Elaeocarpacea) was carried out based on bioassay-guided approach. The methanol leaf extract of M. calabura (MCME) and its partitioned extracts [petroleum ether (MCPE), et...

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Main Author:	Sufian, Adila Sahida
Format:	Thesis
Language:	English
Published:	2013
Online Access:	https://ir.uitm.edu.my/id/eprint/18282/2/TM_ADILA%20SAHIDA%20SUFIAN%20%20PH%2013_5.pdf
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id	my-uitm-ir.18282
record_format	uketd_dc
spelling	my-uitm-ir.182822022-03-01T02:05:42Z Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian 2013 Sufian, Adila Sahida In the present work, isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura (Elaeocarpacea) was carried out based on bioassay-guided approach. The methanol leaf extract of M. calabura (MCME) and its partitioned extracts [petroleum ether (MCPE), ethyl acetate (MCEA) and aqueous (MCAQ)] were subjected to antimicrobial and cytotoxic activities using micro-broth dilution and MTT assays, respectively. MCEA, which appeared to be the most active extract against MSS A (S. aureus 25923) (MIC - 125 μg/mL) and HL60 cell line (human acute promyelocytic leukemia) (IC50 = 17.26 μg/mL), was further fractionated using vacuum liquid chromatography (VLC) to afford seven fractions (F1-F7). 2013 Thesis https://ir.uitm.edu.my/id/eprint/18282/ https://ir.uitm.edu.my/id/eprint/18282/2/TM_ADILA%20SAHIDA%20SUFIAN%20%20PH%2013_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Pharmacy
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
description	In the present work, isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura (Elaeocarpacea) was carried out based on bioassay-guided approach. The methanol leaf extract of M. calabura (MCME) and its partitioned extracts [petroleum ether (MCPE), ethyl acetate (MCEA) and aqueous (MCAQ)] were subjected to antimicrobial and cytotoxic activities using micro-broth dilution and MTT assays, respectively. MCEA, which appeared to be the most active extract against MSS A (S. aureus 25923) (MIC - 125 μg/mL) and HL60 cell line (human acute promyelocytic leukemia) (IC50 = 17.26 μg/mL), was further fractionated using vacuum liquid chromatography (VLC) to afford seven fractions (F1-F7).
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Sufian, Adila Sahida
spellingShingle	Sufian, Adila Sahida Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian
author_facet	Sufian, Adila Sahida
author_sort	Sufian, Adila Sahida
title	Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian
title_short	Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian
title_full	Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian
title_fullStr	Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian
title_full_unstemmed	Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian
title_sort	bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of muntingia calabura / adila sahida sufian
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Pharmacy
publishDate	2013
url	https://ir.uitm.edu.my/id/eprint/18282/2/TM_ADILA%20SAHIDA%20SUFIAN%20%20PH%2013_5.pdf
_version_	1783733650954649600

Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian

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