Bio-guided isolation of antinociceptive compounds from Muntingia calabura l. leaf extract / Mohd Izwan Mohamad Yusof

Muntingia calabura (Elaeocarpaceae) is known as “Buah Ceri Kampung” locally. It has been claimed by the Peruvian folklore to possess medicinal values such as analgesic, which include soothing gastric ulcers, relieving headache and cold and reducing swelling of the prostate gland. This study focuses...

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Main Author:	Mohamad Yusof, Mohd Izwan
Format:	Thesis
Language:	English
Published:	2013
Online Access:	https://ir.uitm.edu.my/id/eprint/18396/2/TM_MOHD%20IZWAN%20MOHAMAD%20YUSOF%20PH%2013_5.pdf
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spelling	my-uitm-ir.183962022-03-01T03:25:09Z Bio-guided isolation of antinociceptive compounds from Muntingia calabura l. leaf extract / Mohd Izwan Mohamad Yusof 2013 Mohamad Yusof, Mohd Izwan Muntingia calabura (Elaeocarpaceae) is known as “Buah Ceri Kampung” locally. It has been claimed by the Peruvian folklore to possess medicinal values such as analgesic, which include soothing gastric ulcers, relieving headache and cold and reducing swelling of the prostate gland. This study focuses on evaluating the antinociceptive effects of the extract of the leaves of Muntingia calabura using formalin-induced licking and biting test. In the present study, activity-guided of the methanol extract of Muntingia calabura (MCME) collected in Shah Alam, Malaysia were evaluated for their antinociceptive properties using the formalin test. Seven fractions of Muntingia calabura petroleum ether extract (MCPE) were labeled as A, B, C, D, E, F and G. The antinociceptive properties of the fractions were studied in comparison to distilled water (dFbO) or 10% Dimethyl Sulfoside (DMSO), morphine and aspirin (ASA) as negative and positive control respectively. Fraction D showed most significant antinociceptive activity when compared to another fraction both in first phase and second phase of formalin test at a dosage of 300 mg/kg. Fraction D and morphine showed no significant differences in first phase, while in second phase, fraction D and aspirin showed no significant differences. Various chromatographic methods were used to separate all compounds including vacuum layer chromatography (VLC), thin layer chromatography (TLC), glass column chromatography, and centrifugal chromatography. One new compound together with three known compounds namely 8-hydroxy-6-methoxyflavone (IC4), 5-hydroxy-3, 6, 7- trimethoxyflavone, (IC1), 3, 7-dimethoxy-5-hydroflavone (IC2) and 2 \ 4’-dihydroxy-3’- methoxychalcone (IC3) were isolated from fraction D respectively. IC3 exhibited higher percentage of antinociception inhibition both in first phase (34.5 %) and second phase (43.8 %) of formalin test at the dose of 50 mg/kg. As a conclusion, the result of the present study support the folkloric use of the leaves of Muntingia calabura in Peruvian folk medicine against pain, and flavonoid derivatives are directly involved in the antinociceptive mechanism of Muntingia calabuura leaves extract. However, further study is required to confirm the exact mechanism involved. 2013 Thesis https://ir.uitm.edu.my/id/eprint/18396/ https://ir.uitm.edu.my/id/eprint/18396/2/TM_MOHD%20IZWAN%20MOHAMAD%20YUSOF%20PH%2013_5.pdf text en public mphil masters Universiti Teknologi MARA Faculty of Pharmacy
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
description	Muntingia calabura (Elaeocarpaceae) is known as “Buah Ceri Kampung” locally. It has been claimed by the Peruvian folklore to possess medicinal values such as analgesic, which include soothing gastric ulcers, relieving headache and cold and reducing swelling of the prostate gland. This study focuses on evaluating the antinociceptive effects of the extract of the leaves of Muntingia calabura using formalin-induced licking and biting test. In the present study, activity-guided of the methanol extract of Muntingia calabura (MCME) collected in Shah Alam, Malaysia were evaluated for their antinociceptive properties using the formalin test. Seven fractions of Muntingia calabura petroleum ether extract (MCPE) were labeled as A, B, C, D, E, F and G. The antinociceptive properties of the fractions were studied in comparison to distilled water (dFbO) or 10% Dimethyl Sulfoside (DMSO), morphine and aspirin (ASA) as negative and positive control respectively. Fraction D showed most significant antinociceptive activity when compared to another fraction both in first phase and second phase of formalin test at a dosage of 300 mg/kg. Fraction D and morphine showed no significant differences in first phase, while in second phase, fraction D and aspirin showed no significant differences. Various chromatographic methods were used to separate all compounds including vacuum layer chromatography (VLC), thin layer chromatography (TLC), glass column chromatography, and centrifugal chromatography. One new compound together with three known compounds namely 8-hydroxy-6-methoxyflavone (IC4), 5-hydroxy-3, 6, 7- trimethoxyflavone, (IC1), 3, 7-dimethoxy-5-hydroflavone (IC2) and 2 \ 4’-dihydroxy-3’- methoxychalcone (IC3) were isolated from fraction D respectively. IC3 exhibited higher percentage of antinociception inhibition both in first phase (34.5 %) and second phase (43.8 %) of formalin test at the dose of 50 mg/kg. As a conclusion, the result of the present study support the folkloric use of the leaves of Muntingia calabura in Peruvian folk medicine against pain, and flavonoid derivatives are directly involved in the antinociceptive mechanism of Muntingia calabuura leaves extract. However, further study is required to confirm the exact mechanism involved.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Mohamad Yusof, Mohd Izwan
spellingShingle	Mohamad Yusof, Mohd Izwan Bio-guided isolation of antinociceptive compounds from Muntingia calabura l. leaf extract / Mohd Izwan Mohamad Yusof
author_facet	Mohamad Yusof, Mohd Izwan
author_sort	Mohamad Yusof, Mohd Izwan
title	Bio-guided isolation of antinociceptive compounds from Muntingia calabura l. leaf extract / Mohd Izwan Mohamad Yusof
title_short	Bio-guided isolation of antinociceptive compounds from Muntingia calabura l. leaf extract / Mohd Izwan Mohamad Yusof
title_full	Bio-guided isolation of antinociceptive compounds from Muntingia calabura l. leaf extract / Mohd Izwan Mohamad Yusof
title_fullStr	Bio-guided isolation of antinociceptive compounds from Muntingia calabura l. leaf extract / Mohd Izwan Mohamad Yusof
title_full_unstemmed	Bio-guided isolation of antinociceptive compounds from Muntingia calabura l. leaf extract / Mohd Izwan Mohamad Yusof
title_sort	bio-guided isolation of antinociceptive compounds from muntingia calabura l. leaf extract / mohd izwan mohamad yusof
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Pharmacy
publishDate	2013
url	https://ir.uitm.edu.my/id/eprint/18396/2/TM_MOHD%20IZWAN%20MOHAMAD%20YUSOF%20PH%2013_5.pdf
_version_	1783733672312045568

Bio-guided isolation of antinociceptive compounds from Muntingia calabura l. leaf extract / Mohd Izwan Mohamad Yusof

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